My Proven 5-Point Fast Track To A Career In Flow

While we normally cover purely technical content for application in your lab, this week’s article is a bit different. I want to talk about a highly effective career option that I know from many years of direct experience: Shared Resource Laboratories (SRLs). Also known as “core facilities,” these research hubs represent a significant investment—by some number of institutions—in personnel and resources.

In an SRL, staff and directors possess advanced training, allowing them to support resident researchers. Working in an SRL can be a really exciting career option for researchers who enjoy: 

  • Contributing to many different projects
  • Working in scientific customer service
  • Doing flow cytometry!

I can tell you firsthand that getting started in an SRL can be daunting. A lot of different professionals or “users” both expect and assume your expertise. They will come to you for answers, and that can be intimidating to a researcher who is just beginning their career.

Some of these questions might be fairly easy to answer, but the truth is that plenty are not. A successful flow cytometry career means staying up to date on the latest information, best practices, top resources, and more.

Thinking about starting a career in flow cytometry? Then you have a fulfilling and engaging science career ahead of you. And to help guide you during this early stage, here are 5 strategies that will accelerate your progress as you make your way into a credible SRL.

1. Study flow cytometry in depth.

To make the most of science technology, education should be your very first step. In flow cytometry, you will need to learn about operating a cytometer, how to gauge fluorochrome brightness, troubleshoot issues with equipment, in experiments, in data analysis…

The list goes on.

So how do you pursue an education of this magnitude? Some options include:

  • Vendor Training Courses (ideal for instrument-specific training)
  • Society Training (ICCS, ISAC, and ESCCA have accessible training materials for researchers at varying levels of experience)
  • Annual Training Courses (a great way to learn new techniques and network with peers & experts in the field)

With so many training materials being put online because of the current pandemic; it’s an excellent time to take a moment to expand your knowledge. 

2. Find (or start) a regional users’ group.

This step can be challenging – finding all the local users of a specific technology is not as easy as introducing yourself to your neighbors. Fortunately, it’s not uncommon for a given area to host an annual meeting for its regional cytometry group. For example, the annual CYTO and ICCS meetings make it easy to find local users and experts in flow cytometry tech. Of course, if there isn’t a regional group event near you, then you might have to start one yourself.  

Watch the Purdue list, Twitter and Linkedin (among others) for announcements for virtual meetings. This may allow you to attend meetings in different regions, exposing you to different people.  

3. Start developing your support network.

In a complex field like flow cytometry, a support network is critical – this is where you can ask questions and get answers without waiting too long. Developing a network is particularly important during the early stages of your career in an SRL.

There are a few excellent resources that deserve space at the top of your list. These include:

  • The Purdue Listserv — A more-than-20-year-old resource with thousands of scientists as members. Purdue Listserv is dedicated to answering your flow cytometry questions. Whether you need to know about sheath fluid, isotype controls, or the fine points of compensation, their extensive database is a reliable source of answers. This list is moderated to provide more focused conversation among users.
  • The Expert Cytometry Mastery Class — My personal project, the Mastery Class is the world’s fastest-growing and most successful flow cytometry training program. We offer an annual and lifetime subscription to a 4-module training course, a live webinar series, weekly emails, bi-weekly blog articles, and more. Members get access to our private discussion group where we discuss training and education topics on a daily basis. Our network represents a huge opportunity for making career connections in the flow universe.
making career connections in the flow universe with Cheeky Excyte Course
  • Linkedin — The professional ‘Facebook’. There are many different articles out there that offer different tips to improve your LinkedIn profile. It doesn’t hurt to get a professional headshot either. Once you have that, start reaching out slowly to other people in SRLs who can serve as contacts. Be sure not to reach out without including a personal statement about yourself and why you’re reaching out (avoid the ‘Please Join My Network’).  
  • Twitter — This is a great platform to learn about others doing flow cytometry and another source of information. You can start by following people you’ve heard of, core facilities, and companies. Share the tweets you find valuable and don’t be afraid to share your thoughts as well. That’s how you can get noticed and build connections and a following. 
  • ISAC SRL Emerging Leadership Program —  is a good program to apply for early in your career. It will provide you with funding and access to more senior members of society. 

4. Seek out a mentor.

There’s no shame in locating a senior scientist with years of wisdom to impart. Your mentor should be a friendly resource for career advice. They can help you solve difficult scientific or technical problems, act as a sounding board for your ideas, and let you in on the secrets that official educational resources might leave out. 

If your mentor is a part of your own institution, they can even help with potential “political” issues that will arise as you move up the career ladder and become the manager or director of a facility.

The Association of Biomolecular Resource Facilities has also established a Mentoring program, which may be another resource to find someone who could serve as mentors.  

As you search for a mentor, make sure that there is chemistry and a fit between you and the mentor. Also, work to establish expectations of the relationship so that everyone goes into the relationship with eyes open. This should be codified using a mentorship agreement such as this one. You should also evaluate the relationship on an annual basis. 

Of course, as you grow in your career, don’t be surprised if you become a mentor to someone new to the field. 

5. Maximize your network (and expertise) with travel.

A fully-rounded education in flow cytometry is hard to come by, especially if expertise/resources are limited at your current institution. That means you’ve probably got some travel ahead of you. Rather than sticking it out at just one location, it’s a better strategy to simply use another institution to learn a specific technique. “Travel” may be more virtual at this time, but don’t hesitate to reach out to other groups to gain information and experience. 

Travel costs money and takes time, but your career will thank you for it. Becoming a member of an SRL is an exciting opportunity, and to be successful in this field, you have to seek out new educational opportunities & network with your peers. If you’re not the networking type, remember that most flow cytometrists are usually happy to talk and share their ideas/experiences to help out a fellow cytometrist. 

To learn more about important control measures for your flow cytometry lab, and to get access to all of our advanced materials including 20 training videos, presentations, workbooks, and private group membership, get on the Flow Cytometry Mastery Class wait list.

Join Expert Cytometry's Mastery Class

ABOUT TIM BUSHNELL, PHD

Tim Bushnell holds a PhD in Biology from the Rensselaer Polytechnic Institute. He is a co-founder of—and didactic mind behind—ExCyte, the world’s leading flow cytometry training company, which organization boasts a veritable library of in-the-lab resources on sequencing, microscopy, and related topics in the life sciences.

Tim Bushnell, PhD

Similar Articles

The Power Of Spectral Viewers And Their Use In Full Spectrum Flow Cytometry

The Power Of Spectral Viewers And Their Use In Full Spectrum Flow Cytometry

By: Tim Bushnell, PhD

What photon from yonder fluorochrome breaks?  It is … umm… hmmm. Let me see. Excitation off a 561 nm laser, with an emission maximum of 692 nm. I’m sure if Shakespeare was a flow cytometrist, he might have written that very scene. But the play is lost in time. However, since the protagonist had difficulty determining what fluorochrome was emitting photons, let’s consider how this could be figured out. In my opinion, one of the handiest flow cytometry tools is the spectral viewer. This tool helps visualize the excitation and emission profile of different fluorochromes, as well as allowing you…

Fickle Markers: Solutions For Antibody Binding Specificity Challenges

Fickle Markers: Solutions For Antibody Binding Specificity Challenges

By: Tim Bushnell, PhD

Reproducibility has been an ongoing, and important, concept in the sciences for years.  In the area of biomedical research, the alarm was sounded by several papers published in the early 2010’s.  Authors like Begley and Ellis, Prinz and coworkers, and Vasilevsky and colleagues, among others reported an alarming trend in the reproducibility of pre-clinical data.  These reports indicated between 50% to almost 90% of published pre-clinical data were not reproducible.  This was further highlighted in the article by Freedman and coworkers, who tried to identify and quantify the different sources of error that could be causing this crisis.  Figure 1,…

5 Common Flow Cytometry Questions, Answered

5 Common Flow Cytometry Questions, Answered

By: Tim Bushnell, PhD

I want to thank all of you who send us your questions about flow cytometry, so I thought I would dip into the old email bag and answer a few of the common ones here.  If your question isn’t answered this time, look for it to be answered in a future blog post.  Of course, if you want us to cover a specific topic, drop us a line.  1. How Fast Can I Go? This is  a common question. The allure of the ‘hi’ button is hard to resist.  The faster you go, the sooner you are finished with data…

Combining Flow Cytometry With Plant Science, Microorganisms, And The Environment

Combining Flow Cytometry With Plant Science, Microorganisms, And The Environment

By: Tim Bushnell, PhD

My first introduction to flow cytometry was talking to a professor who’d brought one on a research cruise to study phytoplankton. It was only later that I was introduced to the marvelous world that’s been my career for over 20 years.   In that time, I’ve had the opportunity to work with researchers in many different areas, exposing me to a wide variety of cell types and more important assays. What continues to amaze me is the number of different parameters we can measure, not just the number of fluorochromes, but the information we can extract from samples – animal, vegetable…

Common Numbers-Based Questions I Get As A Flow Cytometry Core Manager And How To Answer Them

Common Numbers-Based Questions I Get As A Flow Cytometry Core Manager And How To Answer Them

By: Tim Bushnell, PhD

Numbers are all around us.  My personal favorite is ≅1.618 aka ɸ aka ‘the golden ratio’.  It’s found throughout history, where it has influenced architects and artists. We see it in nature, in plants, and it is used in movies to frame shots. It can be approximated by the Fibonacci sequence (another math favorite of mine). However, I have not worked out how to apply this to flow cytometry.  That doesn’t mean numbers aren’t important in flow cytometry. They are central to everything we do, and in this blog, I’m going to flit around numbers-based questions that I have received…

3 Must-Have High-Dimensional Flow Cytometry Controls

3 Must-Have High-Dimensional Flow Cytometry Controls

By: Tim Bushnell, PhD

Developments such as the recent upgrade to the Cytobank analysis platform and the creation of new packages such as Immunocluster are reducing the computational expertise needed to work with high-dimensional flow cytometry datasets. Whether you are a researcher in academia, industry, or government, you may want to take advantage of the reduced barrier to entry to apply high-dimensional flow cytometry in your work. However, you’ll need the right experimental design to access the new transformative insights available through these approaches and avoid wasting the considerable time and money required for performing them. As with all experiments, a good design begins…

The Fluorochrome Less Excited: How To Build A Flow Cytometry Antibody Panel

The Fluorochrome Less Excited: How To Build A Flow Cytometry Antibody Panel

By: Tim Bushnell, PhD

Fluorochrome, antibodies and detectors are important. The journey of a thousand cells starts with a good fluorescent panel. The polychromatic panel is the combination of antibodies and fluorochromes. These will be used during the experiment to answer the biological question of interest. When you only need a few targets, the creation of the panel is relatively straightforward. It’s only when you start to get into more complex panels with multiple fluorochromes that overlap in excitation and emission gets more interesting.  FLUOROCHROMES Both full spectrum and traditional fluorescent flow cytometry rely on measuring the emission of the fluorochromes that are attached…

Flow Cytometry Year in Review: Key Changes To Know

Flow Cytometry Year in Review: Key Changes To Know

By: Meerambika Mishra

Here we are, at the end of an eventful year 2021. But with the promise of a new year 2022 to come. It has been a long year, filled with ups and downs. It is always good to reflect on the past year as we move to the future.  In Memoriam Sir Isaac Newton wrote “If I have seen further, it is by standing upon the shoulders of giants.” In the past year, we have lost some giants of our field including Zbigniew Darzynkiwicz, who contributed much in the areas of cell cycle analysis and apoptosis. Howard Shapiro, known for…

What Star Trek Taught Me About Flow Cytometry

What Star Trek Taught Me About Flow Cytometry

By: Tim Bushnell, PhD

It is no secret that I am a very big fan of the Star Trek franchise. There are many good episodes and lessons explored in the 813+ episodes, 12 movies (and counting). Don’t worry, this blog is not going to review all 813, or even 5 of them. Instead, some of the lessons I have taken away from the show that have applicability to science and flow cytometry.  “Darmok and Jalad at Tanagra.”  (ST:TNG season 5, episode 2) This is probably one of my favorite episodes, which involves Picard and an alien trying to establish a common ground and learn…

Top Industry Career eBooks

Get the Advanced Microscopy eBook

Get the Advanced Microscopy eBook

Heather Brown-Harding, PhD

Learn the best practices and advanced techniques across the diverse fields of microscopy, including instrumentation, experimental setup, image analysis, figure preparation, and more.

Get The Free Modern Flow Cytometry eBook

Get The Free Modern Flow Cytometry eBook

Tim Bushnell, PhD

Learn the best practices of flow cytometry experimentation, data analysis, figure preparation, antibody panel design, instrumentation and more.

Get The Free 4-10 Compensation eBook

Get The Free 4-10 Compensation eBook

Tim Bushnell, PhD

Advanced 4-10 Color Compensation, Learn strategies for designing advanced antibody compensation panels and how to use your compensation matrix to analyze your experimental data.