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Ensuring Reproducibility In Flow Cytometry

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4 Ways To Achieve Reproducible Flow Cytometry Results

Written by Tim Bushnell, Ph.D Reproducibility is one of the hallmarks of the Scientific Method. One reason scientists publish their results is so that other labs can attempt to reproduce the results and extend the investigation into new areas. From the Begley and Ellis commentary in Nature, to the development of the Rigor and Reproducibility initiative at NIH, to articles in the mainstream media, reproducibility is on everyone's mind. This is not a bad thing, and following the best practices in flow cytometry provides investigators, peer reviewers, and colleagues with more confidence in your data. There are several areas within the process of ...

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3 Advantages FCS Express 6 Has Over Other Flow Cytometry Data Analysis Software Programs

Written by Tim Bushnell, Ph.D. After running a flow cytometry experiment and collecting the data, the first and only thing any scientist wants to do is analyze the data. To do this properly in the field of flow cytometry, a quality data analysis software program is required. The problem is, most of the current data analysis programs are archaic, clunky, or overly complex. The package or packages that were useful in the 1990s have not been updated properly and, as a result, are buggy. Worse, they often require a great deal of training to use correctly. However, the biggest problem overall with the ...

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4 Steps To Validate Flow Cytometry Antibodies And Improve Reproducibility

Written by Tim Bushnell, Ph.D Reproducibility is the name of the game in science. For scientific results to be valuable, they must be reproducible. The current crisis in scientific reproducibility has been well-highlighted, and has spurred the NIH to initiate a reproducibility and rigor initiative for grant applications. There is significant concern about the quality and consistency of antibodies in scientific research. Bradbury & Plückthun published a letter in Nature, signed by over 100 researchers, that called for standardizing antibodies by moving away from reliance on traditional monoclonal and polyclonal production towards generation of recombinant antibodies. They estimate that $350 million a year is wasted ...

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Why Understanding Fluorochromes Is Important In Flow Cytometry

Written by Tim Bushnell, Ph.D At the most basic level, a flow cytometer is photon counting device. It captures the emitted photons from fluorochromes present on targets — be they cells, beads, or other particles. These fluorochromes can be attached to antibodies or proteins (like Annexin), free molecules that become fluorescent when bound to a target (DNA dyes), or have different fluorescent characteristics under different biological conditions (Indo-1, JC-1). Fluorescent molecules are the tools of the trade in flow cytometry and, with continued advances in chemistries, it is helpful to step back and review their essential properties. Why Understanding The Jablonski Diagram Is Important When ...

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5 Steps For Accurate Flow Cytometry Statistical Analysis Results

Written by Tim Bushnell, Ph.D At the end of many experiments, the question of statistical analysis rears its ugly head. When it comes up, many researchers freeze not knowing how to proceed, and they muddle through as best they can. With some proper planning and forethought, this doesn’t have to be the case. To resolve this analysis dilemma, it is important to begin thinking about the statistical analysis during the initial designing of the experiments. This is where some critical decisions need to be made to ensure that, if there are statistically significant findings to be uncovered, the data will be sufficient to ...

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WELCOME TO EXCYTE

ExCyte is the world’s leading flow cytometry training company. Whether you are a core manger looking to keep your users on the cutting edge, or a business professional looking to enter into this rapidly growing market, ExCyte can help. We have the information and experience you need.

ExCyte is currently booking beginner and advanced flow cytometry courses around the world. Click on the Calendar box above to see where we will be next.

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Testimonials
MedImmune purchased a site license of Expert Cytometry’s Mastery Class. We have over 100 scientists using the program and the feedback has been phenomenal. I’m amazed at how much the scientists are coming to me with questions that are much more advanced than what I used to get before having access to the Mastery Class. I’m now able to focus my attention, and that of my staff’s, to more advanced questions and inquiries specific to the researcher. We are also having less trouble with the mistakes that new users make and spending less time dealing with troubleshooting basic issues. As the core director at MedImmune, I definitely recommend the Expert Cytometry Mastery Class to all cores, particularly in a corporate setting, where we simply do not have the time to train all of the new users in best practices. My core is running 10 hours a week, ...

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Johnna Wesley, Ph.D.Senior Manager, Novo Nordisk
Expert Cytometry provided an excellent course to our group. The course was over two days. On day one, the instructors provided a great overview of core concepts of flow. On day two, we were able to customize the course to our needs. It was very valuable and I would recommend that if your group is starting to use flow more heavily, that you bring Expert Cytometry in for a course. There are nuances that I never knew and it immediately helped our group perform better experiments.   

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Before taking this course, my experience with flow was limited to simple 2 color experiments. Then I was asked to perform a 10-color complete immunophenotype experiment in non-lymphoid tissues. With a ton of help from a long time co-worker and friend, I was successful with the effort. She highly recommended the Excyte Mastery Course to me and I can say she was right. This course truly elevated my knowledge and understanding of flow cytometry. It was very organized, concise and covered everything from understanding your instrument, optimizing it's performance, designing multicolor panels and executing a flow cytometry experiment with all the necessary controls. I look forward to learning more from the advanced seminars and webinars. I highly recommend this course.

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I was (and still am for the most part) completely ignorant of the details of flow cytometry. The Expert Cytometry team provided explanations and helped me understand the technology to the point where I was able to intelligently and efficiently manage both of our contract flow labs. Expert Cytometry provided independent assessments of the quality of the work and data from the labs that allowed me to make good decisions regarding the results. Finally, I was always amazed at how quickly Expert Cytometry responded and completed the analyses when we were on short timelines. We continue to work with ExCyte and will for the foreseeable future.

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Our group at BlueBirdBio was relatively new to flow. We brought ExCyte in for a basic course, and also to help train us on best practices for our new flow cytometer. The level of flow cytometry knowledge ExCyte brought with them was amazing. I can’t believe how much experience ExCyte has and how much they bring to the courses they provide. ExCyte was able to answer all questions with real world examples. The course brought the theory of flow to the real world and helped us understand the concepts more easily. ExCyte was professional, courteous, and it almost felt like they became part of our group while we were working together. I know I can ask ExCyte any questions whenever I need them and I hope to continue to work with them as we move forward. I have no doubt we’ll bring ExCyte back for another course in ...

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