Written By: Tim Bushnell, Ph.D.
Cell sorting is such an important technique because it's a gateway tool for many other downstream applications.
Assays such as culturing cells, genomic analysis, proteomics, injections into mice and the like are enabled by cell sorting, allowing researchers to use a homogeneously defined population of cells in their experiments.
With single-cell genomics we can readily characterize a population in great detail, but we have to have a single purified population for it to work.
That's where the cell sorter comes in.
There are all sorts of applications we can do with a purified population and with the advances in sorting ...
Written By: Tim Bushnell, Ph.D.
What are the controls that you should be considering when planning and designing your flow cytometry experiments?
Are you using isotype controls? Should you be?
Isotype controls are one of the controls used in flow cytometry today and is very controversial. In fact, I encourage people to not use it.
Are you following the rules of compensation and including the appropriate controls?
Compensation is such an important thing to get correct when running your experiments, especially if you're doing polychromatic flow cytometry, which many of us are doing nowadays.
What types of quality controls should you have in place? Quality control ...
Written by: Tim Bushnell, Ph.D.
I spend a lot of time working with my colleagues and clients retrospectively troubleshooting their data. This involves trying to understand and explain what might've happened during the data acquisition that led to the results in question.
During these discussions, I give them tips and strategies to improve their acquisition and data quality over time because we want to make sure that people have the highest quality data they can. This means that the researchers should understand and follow the best practices and are taking the time to make sure that their data is collected correctly.
There are ...
Written By: Tim Bushnell, Ph.D.
Isaac Newton was famous for saying â€śIf I have seen further than others, it is by standing upon the shoulders of giants.â€ť Implicit in that statement is that the information that the giants provided was reproducible. In fact, reproducibility is central to the scientific method and as far back as the 10th century, the concept of reproducibility of data was being discussed by Ibn al-Haytham.
In 2011, Prinz et al. published an article that indicated a case study looking at reproducibility by Bayer Healthcare found only 25% of academic studies were reproducible. This was followed up in ...
The Power Of Flow Cytometry In Cell Sorting
Often, cell sorting is described as trying to find a needle in a haystack. In 2008, David Brown wrote an article on cell sorting for the Washington Post. In that article, he wrote :
â€śFinding and sorting a few cells from a great mass of them is sometimes likened to finding a needle in a haystack. But it's actually more watching a parade of 10,000 undertakers, and spotting the two who are wearing yellow ties, and the three who are wearing pink ties, and getting them out of the line, without making everyone else ...
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MedImmune purchased a site license of Expert Cytometryâ€™s Mastery Class. We have over 100 scientists using the program and the feedback has been phenomenal. Iâ€™m amazed at how much the scientists are coming to me with questions that are much more advanced than what I used to get before having access to the Mastery Class. Iâ€™m now able to focus my attention, and that of my staffâ€™s, to more advanced questions and inquiries specific to the researcher. We are also having less trouble with the mistakes that new users make and spending less time dealing with troubleshooting basic issues.Â As the core director at MedImmune, I definitely recommend the Expert Cytometry Mastery Class to all cores, particularly in a corporate setting, where we simply do not have the time to train all of the new users in best practices. My core is running 10 hours a week, ...
Expert CytometryÂ provided an excellent course to our group. The course was over two days. On day one, the instructors provided a great overview of core concepts of flow. On day two, we were able to customize the course to our needs. It was very valuable and I would recommend that if your group is starting to use flow more heavily, that you bring Expert Cytometry in for a course. There are nuances that I never knew and it immediately helped our group perform better experiments. Â Â
Before taking this course, my experience with flow was limited to simple 2 color experiments. Then I was asked to perform a 10-color complete immunophenotype experiment in non-lymphoid tissues. With a ton of help from a long time co-worker and friend, I was successful with the effort. She highly recommended the Excyte Mastery Course to me and I can say she was right. This course truly elevated my knowledge and understanding of flow cytometry. It was very organized, concise and covered everything from understanding your instrument, optimizing it's performance, designing multicolor panels and executing a flow cytometry experiment with all the necessary controls. I look forward to learning more from the advanced seminars and webinars. I highly recommend this course.
I was (and still am for the most part) completely ignorant of the details of flow cytometry.Â The Expert Cytometry teamÂ provided explanations and helped me understand the technology to the point where I was able to intelligently and efficiently manage both of our contract flow labs. Expert Cytometry provided independent assessments of the quality of the work and data from the labs that allowed me to make good decisions regarding the results. Finally, I was always amazed at how quickly Expert CytometryÂ responded and completed the analyses when we were on short timelines. We continue to work with ExCyte and will for the foreseeable future.
Our group at BlueBirdBio was relatively new to flow. We brought ExCyte in for a basic course, and also to help train us on best practices for our new flow cytometer. The level of flow cytometry knowledge ExCyte brought with them was amazing. I canâ€™t believe how much experience ExCyte has and how much they bring to the courses they provide. ExCyte was able to answer all questions with real world examples. The course brought the theory of flow to the real world and helped us understand the concepts more easily. ExCyte was professional, courteous, and it almost felt like they became part of our group while we were working together. I know I can ask ExCyte any questions whenever I need them and I hope to continue to work with them as we move forward. I have no doubt weâ€™ll bring ExCyte back for another course in ...