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Ensuring Reproducibility In Flow Cytometry

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The Right Way To Read A Flow Cytometry Scientific Paper

Written By: Tim Bushnell, Ph.D. Scientific reproducibility and the public's confidence in scientific results is critically important. One has to look no further than the website Retraction Watch to learn of the published papers that are being retracted for a host of reasons. Reasons for retractions can range from plagiarism to scientific misconduct. The cost of these retractions is more than losing a paper, it can lead to financial penalties and even prison. In March, 2019 Duke University settled a lawsuit brought by the US government for over 100 million dollars arising from fraudulent data being used by a researcher in ...

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Avoid Data Loss By Following These Steps To Set Your Flow Cytometry Gates Correctly

Written By: Tim Bushnell, Ph.D. How are you defining your gates? When you do primary analysis or single tube analysis, it’s important that you make sure your gates are set correctly. You need to know that you can find the populations that you're interested in so you can extract the appropriate data. This is what allows you to do your secondary or statistical analysis confidently. What Gates Should You Be Setting Up In Your Flow Experiments At the beginning of the experimental design process, it is a good idea to sketch out a hypothetical gating strategy. This sketch will help evaluate the panel and ensure that ...

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3 Ways To Measure Cell Death With Flow Cytometry

Written By: Tim Bushnell, Ph.D. Cell death is a natural part of the lifecycle of a cell. In cases of development, it is critical for the shaping of fingers during human development. The processes of ordered cell death, or Apoptosis, are so important that in 2002, Sidney Brenner, Robert Horvitz, and John Sulston received the Nobel Prize in Medicine for their work on understanding this process. There are many different ways to measure cell death and flow cytometry is an ideal tool for this technique. Whether you are just assessing the viability of your cells or you are interested in the ...

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5 Essential Controls For Reproducible Fluorescent Microscopy Imaging

Written By: Heather Brown Harding, Ph.D. Fluorescent imaging has the potential to bring great insights into your research project. But, there is a lot of room for error. And one of the most common and completely preventable errors that many researchers make is not having the necessary controls. Having all the necessary controls may seem tedious. I know in graduate school I was not a huge fan of controls, and would sometimes put off doing all the controls I needed until the end. However, doing your controls after the fact is a terrible idea. You could realize that all the data you recorded was just an ...

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3 Action Steps You Can Take Right Now To Improve Your Flow Cytometry Reproducibility

Written By: Tim Bushnell, Ph.D. Reproducibility is a key issue in science. Massive amounts of time and money are wasted when the results of experiments are not reproducible. For example, I was called into a lab to look at their data because they had spent thousands of dollars sorting precious human samples and were now doing genomics analysis with the isolated cells. Unfortunately, the results of the genomics analysis made no sense based on the sorted populations. The lab was working backward through every step of the process to try to identify what might have happened and if the experiments were salvageable. As I reviewed ...

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ExCyte is the world’s leading flow cytometry training company. Whether you are a core manger looking to keep your users on the cutting edge, or a business professional looking to enter into this rapidly growing market, ExCyte can help. We have the information and experience you need.

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MedImmune purchased a site license of Expert Cytometry’s Mastery Class. We have over 100 scientists using the program and the feedback has been phenomenal. I’m amazed at how much the scientists are coming to me with questions that are much more advanced than what I used to get before having access to the Mastery Class. I’m now able to focus my attention, and that of my staff’s, to more advanced questions and inquiries specific to the researcher. We are also having less trouble with the mistakes that new users make and spending less time dealing with troubleshooting basic issues. As the core director at MedImmune, I definitely recommend the Expert Cytometry Mastery Class to all cores, particularly in a corporate setting, where we simply do not have the time to train all of the new users in best practices. My core is running 10 hours a week, ...

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Johnna Wesley, Ph.D.Senior Manager, Novo Nordisk
Expert Cytometry provided an excellent course to our group. The course was over two days. On day one, the instructors provided a great overview of core concepts of flow. On day two, we were able to customize the course to our needs. It was very valuable and I would recommend that if your group is starting to use flow more heavily, that you bring Expert Cytometry in for a course. There are nuances that I never knew and it immediately helped our group perform better experiments.   

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Before taking this course, my experience with flow was limited to simple 2 color experiments. Then I was asked to perform a 10-color complete immunophenotype experiment in non-lymphoid tissues. With a ton of help from a long time co-worker and friend, I was successful with the effort. She highly recommended the Excyte Mastery Course to me and I can say she was right. This course truly elevated my knowledge and understanding of flow cytometry. It was very organized, concise and covered everything from understanding your instrument, optimizing it's performance, designing multicolor panels and executing a flow cytometry experiment with all the necessary controls. I look forward to learning more from the advanced seminars and webinars. I highly recommend this course.

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I was (and still am for the most part) completely ignorant of the details of flow cytometry. The Expert Cytometry team provided explanations and helped me understand the technology to the point where I was able to intelligently and efficiently manage both of our contract flow labs. Expert Cytometry provided independent assessments of the quality of the work and data from the labs that allowed me to make good decisions regarding the results. Finally, I was always amazed at how quickly Expert Cytometry responded and completed the analyses when we were on short timelines. We continue to work with ExCyte and will for the foreseeable future.

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Our group at BlueBirdBio was relatively new to flow. We brought ExCyte in for a basic course, and also to help train us on best practices for our new flow cytometer. The level of flow cytometry knowledge ExCyte brought with them was amazing. I can’t believe how much experience ExCyte has and how much they bring to the courses they provide. ExCyte was able to answer all questions with real world examples. The course brought the theory of flow to the real world and helped us understand the concepts more easily. ExCyte was professional, courteous, and it almost felt like they became part of our group while we were working together. I know I can ask ExCyte any questions whenever I need them and I hope to continue to work with them as we move forward. I have no doubt we’ll bring ExCyte back for another course in ...

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