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Ensuring Reproducibility In Flow Cytometry

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5 FlowJo Hacks To Boost The Quality Of Your Flow Cytometry Analysis

Written By: Tim Bushnell, Ph.D. Primary data analysis, that is the analysis at the sample or tube level, is where the populations of interest are identified and the necessary data is extracted for secondary analysis. Since the creation of the FCS standard, flow cytometrists have had the ability to analyze data in third party software because of the communities agreement on the standard. The most recent standard of FCS3.1. The FCS standard divides the file into two components, the listmode file that contains the sequential data from all the detectors. The header file contains what are termed ‘keywords’. These include define ...

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5 Special Considerations for Live Cell Imaging

Written By: Heather Brown-Harding, Ph.D. Live cell imaging is advantageous for research were you may be worried about artifacts of fixation or when you want to measure a phenomenon over time. Live cell imaging is more difficult to achieve than fixed samples because we need to keep the cells live AND happy along with obtaining the images we need. We can reduce artifacts by keeping the cells in a favorable environment and minimizing external stressors. Here are 5 points to keep in mind when setting up your live cell imaging experiment. 1. Environmental controls. A specific and controlled environment needs to be created ...

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5 Steps To Improve Your Flow Cytometry Data Analysis

Written By: Tim Bushnell, Ph.D. With the continued emphasis on improving the reproducibility of scientific data, it is critical to remember that there is no single step that will solve this problem. Instead, it is a mindset that needs to be adopted. From adding in additional descriptors in the data to ensuring the proper information is extracted these steps, especially when communicated, can improve the robustness of your data. Further, as more and more automated analytical tools are being developed, using Improving the quality of your data starts with how you approach your data analysis process, your experimental design and what happens ...

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The Right Way To Read A Flow Cytometry Scientific Paper

Written By: Tim Bushnell, Ph.D. Scientific reproducibility and the public's confidence in scientific results is critically important. One has to look no further than the website Retraction Watch to learn of the published papers that are being retracted for a host of reasons. Reasons for retractions can range from plagiarism to scientific misconduct. The cost of these retractions is more than losing a paper, it can lead to financial penalties and even prison. In March, 2019 Duke University settled a lawsuit brought by the US government for over 100 million dollars arising from fraudulent data being used by a researcher in ...

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Avoid Data Loss By Following These Steps To Set Your Flow Cytometry Gates Correctly

Written By: Tim Bushnell, Ph.D. How are you defining your gates? When you do primary analysis or single tube analysis, it’s important that you make sure your gates are set correctly. You need to know that you can find the populations that you're interested in so you can extract the appropriate data. This is what allows you to do your secondary or statistical analysis confidently. What Gates Should You Be Setting Up In Your Flow Experiments At the beginning of the experimental design process, it is a good idea to sketch out a hypothetical gating strategy. This sketch will help evaluate the panel and ensure that ...

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ExCyte is the world’s leading flow cytometry training company. Whether you are a core manger looking to keep your users on the cutting edge, or a business professional looking to enter into this rapidly growing market, ExCyte can help. We have the information and experience you need.

ExCyte is currently booking beginner and advanced flow cytometry courses around the world. Click on the Calendar box above to see where we will be next.

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Testimonials
MedImmune purchased a site license of Expert Cytometry’s Mastery Class. We have over 100 scientists using the program and the feedback has been phenomenal. I’m amazed at how much the scientists are coming to me with questions that are much more advanced than what I used to get before having access to the Mastery Class. I’m now able to focus my attention, and that of my staff’s, to more advanced questions and inquiries specific to the researcher. We are also having less trouble with the mistakes that new users make and spending less time dealing with troubleshooting basic issues. As the core director at MedImmune, I definitely recommend the Expert Cytometry Mastery Class to all cores, particularly in a corporate setting, where we simply do not have the time to train all of the new users in best practices. My core is running 10 hours a week, ...

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Johnna Wesley, Ph.D.Senior Manager, Novo Nordisk
Expert Cytometry provided an excellent course to our group. The course was over two days. On day one, the instructors provided a great overview of core concepts of flow. On day two, we were able to customize the course to our needs. It was very valuable and I would recommend that if your group is starting to use flow more heavily, that you bring Expert Cytometry in for a course. There are nuances that I never knew and it immediately helped our group perform better experiments.   

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Before taking this course, my experience with flow was limited to simple 2 color experiments. Then I was asked to perform a 10-color complete immunophenotype experiment in non-lymphoid tissues. With a ton of help from a long time co-worker and friend, I was successful with the effort. She highly recommended the Excyte Mastery Course to me and I can say she was right. This course truly elevated my knowledge and understanding of flow cytometry. It was very organized, concise and covered everything from understanding your instrument, optimizing it's performance, designing multicolor panels and executing a flow cytometry experiment with all the necessary controls. I look forward to learning more from the advanced seminars and webinars. I highly recommend this course.

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I was (and still am for the most part) completely ignorant of the details of flow cytometry. The Expert Cytometry team provided explanations and helped me understand the technology to the point where I was able to intelligently and efficiently manage both of our contract flow labs. Expert Cytometry provided independent assessments of the quality of the work and data from the labs that allowed me to make good decisions regarding the results. Finally, I was always amazed at how quickly Expert Cytometry responded and completed the analyses when we were on short timelines. We continue to work with ExCyte and will for the foreseeable future.

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Our group at BlueBirdBio was relatively new to flow. We brought ExCyte in for a basic course, and also to help train us on best practices for our new flow cytometer. The level of flow cytometry knowledge ExCyte brought with them was amazing. I can’t believe how much experience ExCyte has and how much they bring to the courses they provide. ExCyte was able to answer all questions with real world examples. The course brought the theory of flow to the real world and helped us understand the concepts more easily. ExCyte was professional, courteous, and it almost felt like they became part of our group while we were working together. I know I can ask ExCyte any questions whenever I need them and I hope to continue to work with them as we move forward. I have no doubt we’ll bring ExCyte back for another course in ...

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