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Ensuring Reproducibility In Flow Cytometry

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Ask These 7 Questions Before Purchasing A Flow Cytometer

Written By: Tim Bushnell, Ph.D. Using funds to make a capital purchase can be an exciting time in a facility. If you don’t have the funds in hand, planning for future purchases requires due diligence to make sure the investment is worth it, and that it will satisfy the needs of the community. That $200,000 or more (sometimes much more) is an investment in the future research capabilities of the facility, so invest wisely. Over the course of writing instrumentation grants, developing business plans, and acquiring instruments, the following questions should be your go-to checklist — what I look at and what ...

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6 Microscopy Assays To Determine Cell Health and Improve Your Experimental Results

Written By: Heather Brown-Harding, Ph.D. When you're performing imaging, we always want to make sure that any phenotype we see isn't just an artifact of unhealthy cells or if you're doing drug discovery, you want to ensure that the treatment isn't highly toxic to non-target cells. Therefore, it's important to understand the health of your cells. To begin to understand if your cells are healthy you need to ask yourself: Is the cell still dividing? Is the metabolism of the cell changed? Are these features of apoptosis or necrosis? Are the cells dead or on the verge of dying? Western Blot and flow ...

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7 Things You Didn’t Know About Imaging Cytometry

Written By: Tim Bushnell, Ph.D. We are visual creatures. We seek to capture and describe the world around us. Some of the earliest evidence of this is found in the cave paintings that have been found around the world, like this painting of a horse found in the caves in Lascaux, France. Figure 1: Cave painting of a horse. From Wiki. With the development of reliable microscopes, such as those developed by the dutch draper Antonie van Leeuwenhoek. For the first time, we were able to see what we could not see before, probing the unseen and learning in great detail how organisms ...

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4 Steps To Implementing a QC Program For Your Flow Cytometry Experiments

Written By: Tim Bushnell, Ph.D. With the emphasis on reproducibility in science, it is important to look at the process of quality control and quality assurance and how these can help improve the reproducibility of data. First, what is the difference between quality control (QC) and quality assurance (QA)? For the purposes of this article, we will define QC as the activities that are performed to ensure that the product, in this case, flow cytometry data, meets the standard. QA, on the other hand, is focused on how the processes used to develop the product and how these can be improved as ...

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5 FlowJo Hacks To Boost The Quality Of Your Flow Cytometry Analysis

Written By: Tim Bushnell, Ph.D. Primary data analysis, that is the analysis at the sample or tube level, is where the populations of interest are identified and the necessary data is extracted for secondary analysis. Since the creation of the FCS standard, flow cytometrists have had the ability to analyze data in third party software because of the communities agreement on the standard. The most recent standard of FCS3.1. The FCS standard divides the file into two components, the listmode file that contains the sequential data from all the detectors. The header file contains what are termed ‘keywords’. These include define ...

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WELCOME TO EXCYTE

ExCyte is the world’s leading flow cytometry training company. Whether you are a core manger looking to keep your users on the cutting edge, or a business professional looking to enter into this rapidly growing market, ExCyte can help. We have the information and experience you need.

ExCyte is currently booking beginner and advanced flow cytometry courses around the world. Click on the Calendar box above to see where we will be next.

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Testimonials
MedImmune purchased a site license of Expert Cytometry’s Mastery Class. We have over 100 scientists using the program and the feedback has been phenomenal. I’m amazed at how much the scientists are coming to me with questions that are much more advanced than what I used to get before having access to the Mastery Class. I’m now able to focus my attention, and that of my staff’s, to more advanced questions and inquiries specific to the researcher. We are also having less trouble with the mistakes that new users make and spending less time dealing with troubleshooting basic issues. As the core director at MedImmune, I definitely recommend the Expert Cytometry Mastery Class to all cores, particularly in a corporate setting, where we simply do not have the time to train all of the new users in best practices. My core is running 10 hours a week, ...

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Johnna Wesley, Ph.D.Senior Manager, Novo Nordisk
Expert Cytometry provided an excellent course to our group. The course was over two days. On day one, the instructors provided a great overview of core concepts of flow. On day two, we were able to customize the course to our needs. It was very valuable and I would recommend that if your group is starting to use flow more heavily, that you bring Expert Cytometry in for a course. There are nuances that I never knew and it immediately helped our group perform better experiments.   

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Before taking this course, my experience with flow was limited to simple 2 color experiments. Then I was asked to perform a 10-color complete immunophenotype experiment in non-lymphoid tissues. With a ton of help from a long time co-worker and friend, I was successful with the effort. She highly recommended the Excyte Mastery Course to me and I can say she was right. This course truly elevated my knowledge and understanding of flow cytometry. It was very organized, concise and covered everything from understanding your instrument, optimizing it's performance, designing multicolor panels and executing a flow cytometry experiment with all the necessary controls. I look forward to learning more from the advanced seminars and webinars. I highly recommend this course.

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I was (and still am for the most part) completely ignorant of the details of flow cytometry. The Expert Cytometry team provided explanations and helped me understand the technology to the point where I was able to intelligently and efficiently manage both of our contract flow labs. Expert Cytometry provided independent assessments of the quality of the work and data from the labs that allowed me to make good decisions regarding the results. Finally, I was always amazed at how quickly Expert Cytometry responded and completed the analyses when we were on short timelines. We continue to work with ExCyte and will for the foreseeable future.

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Our group at BlueBirdBio was relatively new to flow. We brought ExCyte in for a basic course, and also to help train us on best practices for our new flow cytometer. The level of flow cytometry knowledge ExCyte brought with them was amazing. I can’t believe how much experience ExCyte has and how much they bring to the courses they provide. ExCyte was able to answer all questions with real world examples. The course brought the theory of flow to the real world and helped us understand the concepts more easily. ExCyte was professional, courteous, and it almost felt like they became part of our group while we were working together. I know I can ask ExCyte any questions whenever I need them and I hope to continue to work with them as we move forward. I have no doubt we’ll bring ExCyte back for another course in ...

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