Ask These 7 Questions Before Purchasing A Flow Cytometer
I am still convinced that my first cell sorter was possessed. The number of issues that I had with the system remains hard for me to believe, even after all these years.
It had been purchased, in part, from one vendor because the sales rep for a competitor was nowhere to be found. At that time, I admit I wasn’t overly diligent in my research process. Since then, I’ve pinpointed some critical questions that need to be answered before purchasing a new instrument.
At the end of the process, a shiny new instrument will arrive at your facility. Make sure you find time to do a shakedown and validate the system. This is the time to get to know it better, identify quirks and potential issues, and develop training and QC programs. Once your shakedown is complete, you can start adding users and encouraging feedback on the system.
6 Microscopy Assays To Determine Cell Health and Improve Your Experimental Results
When you’re performing imaging, always make sure that any phenotype isn’t just an artifact of unhealthy cells. If you’re doing drug discovery, you want to ensure that the treatment isn’t highly toxic to non-target cells. Therefore, it’s important to understand the health of the cells.Read More
7 Things You Didn’t Know About Imaging Cytometry
It has been said that “a picture is worth a thousand words.” We are visual creatures, and we seek to capture and describe the world around us. Some of the earliest evidence for this comes from very old cave paintings found around the world, like this painting of a horse found in the caves in Lascaux, France.
With the development of reliable microscopes, such as those developed by the dutch draper Antonie van Leeuwenhoek, we were able to see what was previously invisible, probing the unseen and learning in great detail how organisms worked.
Over time, the field of cytometry (the analysis of biological processes at the whole-cell level) has expanded in many different directions. Flow cytometry can be thought of as a microscope with very poor resolution. The power of flow cytometry lies in its ability to analyze thousands of cells through many dimensions, providing an amazingly detailed understanding of the cell. However, due to the resolution, it is not possible to tell where these signals are located.Read More
4 Steps To Implementing a QC Program For Your Flow Cytometry Experiments
Quality control is the hallmark of improving reproducibility. QC programs are designed to help determine when the process in question goes off the expected path. Depending on the deviation from the established acceptance criteria will dictate the level of intervention that needs to occur. This can be as easy as cleaning the instrument and rerunning the QC, or as extreme as removing the data from the final analysis. Since there is documentation as to the deviations, this provides the rationale for excluding data.Read More
5 FlowJo Hacks To Boost The Quality Of Your Flow Cytometry Analysis
FlowJo is a powerful tool for performing and analyzing flow cytometry experiments, if you know how to use it to the fullest. This includes understanding embedding and using keywords, the FlowJo compensation wizard, spillover spreading matrix, FlowJo and R, and creating tables in FlowJo. Extending your use of FJ using these hacks will help organize your data, improve analysis and make your exported data easier to understand and explain to others. Take a few moments and explore all you can do with FJ beyond just gating populations.Read More