In the late 1990s, a group of research scientists founded Cell Signaling Technology (CST) to develop antibodies for applied systems biology research and to provide support to researchers. CST develops primary antibodies, secondary antibodies, antibody conjugates, ELISA, and antibody arrays. The company also tests these antibodies in-house. The tests are to confirm reproducibility, specificity, and sensitivity are at acceptable levels. These antibodies are suitable for a range of applications that include western blotting and flow cytometry.
The primary antibodies include Akt, ALK, Caspase, MAPK, mTOR, and NF-kB. Akt antibodies are more commonly referred to as PKB, and is a key factor in various cellular processes. These processes include insulin metabolism and cancer progression. The Akt antibody exists in three isoforms. The class of Caspase products are essential for regulating apoptosis. The Initiator caspases are used for cleaving and activating effector caspases that exist downstream. This causes apoptosis to execute. Cell Signaling Technology offers several products for MAP/Erk, which include antibodies for both total and cleaved protein, control botinylated and blocking peptides, and activity assay kits. The company also produces antibody sampler kits and PathScan® ELISA kits and antibody Pairs.
The antibody sampler kits that Cell Signaling Technology offers are comprised of multiple antibodies that are specific to particular cellular process, pathway, or protein. These sample-size antibodies are also specific to secondary antibodies. Researchers are able to use each primary antibody sample to complete four western mini-blots for each target.
Another type of kit that CST provides is the PathScan® Antibody Array Kit. The assays in these kits are slide-based. These assays utilize the ELISA principle. These assays are suitable for researching the transduction of signals that occur through key pathway nodes that are in a multiplex format. Researchers can obtain the PathScan Antibody Array kits to use for fluorescent detection or chemilluminescent detection.
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My other passions include grilling, wine tasting, and real food. To be honest, my biggest passion is flow cytometry, which is something that Carol and I share. My personal mission is to make flow cytometry education accessible, relevant, and fun. I’ve had a long history in the field starting all the way back in graduate school.