Beckman Coulter offers several flow cytometers. The Gallios™ is a system is designed to acquire as many as 10 colors. The system also includes an optical system that enables it to handle multicolor assays. There are several other features of the Gallios instrument.
The Gallios offers four laser configurations. The six-color configuration includes 2 lasers consisting of Blue 488 nm and Red 638 nm). The eight-color configuration consists of 2 lasers that are Blue 488 and Red 638. The 10-color configuration includes 3 lasers of Blue 488 nm, Red 638 nm, and Violet 405 nm). The system includes another 10-color configuration with 3 lasers. This configuration includes Blue 488 nm, Yellow 561 nm, Red 638 nm, and Violet 405 nm. The Yellow laser is co-linear.
The scatter detection feature of the Gallios system is unique. The forward-scatter detector is able to perform up to three cell-size measurements at a very low level. The system also includes side scatter detection that offers electronic attenuation. The system also includes a total of six fluorescence detectors that are able to acquire a maximum of six fluorescence signals. The operator has the option of adding a total of four fluorescence detectors to the instrument, which bumps up the number of colors able to be read to 10.
For each cell analysis process, an operator of the Gallios is able to select as many as 62 parameters. The rate of acquisition of these cells is 25,000 events per second.
The software program that is integrated with the Gallios allows multiple operators to administer the Gallios at one time. The software also offers Boolean gating and provides a color eventing hierarchy. The system provides an automatic PDF and .xls generation feature.
The Gallios offers a plug-and-play option for filters that provides customer-specific temperature regulation. In addition, the operator is able to exchange filters without the use of any mechanical tools.
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My other passions include grilling, wine tasting, and real food. To be honest, my biggest passion is flow cytometry, which is something that Carol and I share. My personal mission is to make flow cytometry education accessible, relevant, and fun. I’ve had a long history in the field starting all the way back in graduate school.