Complex Data Analysis with FlowJo Labs at UCSF | Expert Cytometry | Flow Cytometry Training Complex Data Analysis with FlowJo Labs at UCSF | Expert Cytometry | Flow Cytometry Training
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Complex Data Analysis with FlowJo Labs at UCSF

September 23, 2014 @ 9:00 AM - September 24, 2014 @ 5:00 PM

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Educational Objectives

At the end of this two day course, students will have a better understanding of the principles of data analysis. This knowledge will include principles on obtaining good data, the use of controls for data analysis, statistical analysis of flow cytometric data as well as advanced topics including proliferation, cluster analysis and the use of SPICE, a separate (and free) analysis package for analyzing complex datasets.

Target Audience

Researchers using flow cytometry in their work and at least 6 months of experience in cytometry. Basic knowledge of flow cytometry and Flowjo are expected.

Course Outline/Schedule – Day 1

09:00‐09:30 Instructor introductions and course outline

09:30‐10:30 Generating good data (Lecture 1)

10:30‐10:45 Coffee Break

10:45‐11:45 Introduction to FlowJo (Lab 1: Dataset – 1 color titration)

11:45‐12:00 Q/A from morning

12:00‐13:00 Lunch break

13:00‐14:00 Principles of Compensation (Lecture 2)

14:00‐15:00 Compensation in FlowJo (Lab 2: Dataset ‐ 5 color panel)

15:00‐15:15 Break

15:15‐16:00 Principles of Data Analysis (Lecture 3)

16:00‐17:00 Practical Data Analysis (Lab 3: Dataset – 5 color panel)

Course Outline/Schedule – Day 2

09:00‐10:15 Statistical Analysis of Flow data (Lecture 3)

10:15‐10:30 Coffee Break

10:30‐12:00 Advanced Data analysis (Lab 4: Dataset – 10 color stimulation)

12:00‐13:00 Lunch break

13:00‐14:00 Advanced Data Analysis – Data Modeling and SPICE (Lecture 4)

14:00‐15:00 Modeling lab 1 (Lab 5: Dataset – Cell Cycle and Proliferation)

15:00‐15:15 Break

15:15‐16:00 Modeling lab 2 (Lab 6: Dataset – SPICE Dataset)

16:00‐16:45 Presenting good data (Lecture 5)

16:45‐17:00 Q/A and wrap‐up



Tim Bushnell

Tim Bushnell

I enjoy answering paradigm-shifting questions and trouble-shooting puzzling glitches. I also like finding new ways to enhance old procedures. I’m passionate about my professional relationships and strive to fill them with positive energy.

My other passions include grilling, wine tasting, and real food. To be honest, my biggest passion is flow cytometry, which is something that Carol and I share. My personal mission is to make flow cytometry education accessible, relevant, and fun. I’ve had a long history in the field starting all the way back in graduate school.
Tim Bushnell


September 23, 2014 9:00 AM
September 24, 2014 5:00 PM
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Parnassus Flow Cytometry Core at UCSF
(415) 430-7676
513 Parnassus Ave., San Francisco, CA 94143 United States
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Flow Cytometry Education And Consulting - Affordable. Effective. Leading Edge.