Sheath fluid is the solution that runs in a flow cytometer. Once the sheath fluid is running at laminar flow, the cells are injected into the center of the stream, at a slightly higher pressure. The principles of hydrodynamic focusing cause the cells to align, single file in the direction of flow.
Depending on experimental needs, different formulations of sheath fluid can be used. Many labs purchase pre-mixed phosphate buffered saline from Leinco Technologies.
Some researchers use a Hepes-buffered saline. This particular useful for high-pressure cell sorting as Hepes controls pH better at high pressure than phosphate buffers do.
Finally, since the sheath and sample core stream do not mix, on analyzers, you can use water as a sheath fluid. Adding a small amount (0.1%) of 2-phenoxyethanol will help as this serves as a surfactant, helping keep the system flowing by reducing the surface tension.
My other passions include grilling, wine tasting, and real food. To be honest, my biggest passion is flow cytometry, which is something that Carol and I share. My personal mission is to make flow cytometry education accessible, relevant, and fun. I’ve had a long history in the field starting all the way back in graduate school.
Latest posts by Tim Bushnell (see all)
- 3 Action Steps You Can Take Right Now To Improve Your Flow Cytometry Reproducibility - April 11, 2019
- 3 Considerations To Ensure Your Cell Sorting Flow Cytometry Experiments Run Smoothly - March 14, 2019
- 3 Questions You Should Be Asking About Flow Cytometry Controls For Your Experiments - February 28, 2019