What is autofluorescence? Autofluorescence is the term given to describe the natural fluorescence that occurs in cells. The common compounds that give rise to this fluorescence signal include cyclic ring compounds like NAD(P)H, Collagen, and Riboflavin, as well as aromatic amino acids including tyrosine, tryptophan, phenylalanine.
These compounds absorb in UV to Blue range (355-488 nm), and emit in the Blue to Green range (350-550 nm). The consequence of this autofluorescence is the loss of signal resolution in these light ranges and a decrease in signal sensitivity.
Autofluorescence typically increases with cell size. Larger cells have more autofluorescence than small cells due to the simple fact that the larger cells often contain more autofluorescent compounds.
My other passions include grilling, wine tasting, and real food. To be honest, my biggest passion is flow cytometry, which is something that Carol and I share. My personal mission is to make flow cytometry education accessible, relevant, and fun. I’ve had a long history in the field starting all the way back in graduate school.
Latest posts by Tim Bushnell (see all)
- Ask These 7 Questions Before Purchasing A Flow Cytometer - September 12, 2019
- 7 Things You Didn’t Know About Imaging Cytometry - August 15, 2019
- 4 Steps To Implementing a QC Program For Your Flow Cytometry Experiments - August 1, 2019