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4 Steps To Validate Flow Cytometry Antibodies And Improve Reproducibility

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Until we have access to well-validated recombinant antibodies produced under tightly regulated conditions, researchers need to exercise good judgment regarding these critical biological reagents. These 4 steps will help ensure that your results are consistent and reproducible. This will both reassure your reviewers that your data is of high quality, and allow for researchers at other institutions to successfully replicate your results. In addition, identifying antibody duds early on will save you time and money in the long run. Don’t shirk the work of ensuring your antibodies are working correctly and targeting the right proteins.

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Why Understanding Fluorochromes Is Important In Flow Cytometry

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Considerations that must be made when choosing fluorochromes include the brightness of the dyes in question, the instrument configuration, and the staining protocol. Each of these factors will impact the quality of the data because of issues related to spectral spillover, staining, loss of signal because of tandem dye degradation, the ability to get an antibody/fluorochrome into a cell, and more. It takes time and effort to develop and optimize a panel. If one fluorochrome doesn’t work, consider why it may have failed and look for alternatives.

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5 Steps For Accurate Flow Cytometry Statistical Analysis Results

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It is critical to prepare for your statistical analysis at the beginning of the experimental design process. This will ensure the correct data is extracted, the proper test applied, and that sufficient replicates are obtained so that if an effect is to be found, it will be found. Here are five considerations to implement into your experimental design to ensure the best statistical methods so that your data stands up to review.

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How To Differentiate T Cell State With Flow Cytometry

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T cell differentiation and effector function regulation is an area that needs to be better understood. Until then, speculation combined with best practices help you determine your T cell population’s status. Functional profiling is the primary determinant with surface markers as supporting evidence to assess whether your T cell is activated, resting, or naïve. Here are ways to help differentiate your T cells.

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How To Get A Flow Cytometry Job In 5 Steps

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Flow cytometry is a powerful technique impacting both clinical and research. When looking for a career, flow cytometry can take you many places. An experienced flow cytometrist can find a job in a biotechnology company, academia, a clinical setting, and more. To be successful in the field, it’s important to seek out new educational opportunities and network with your peers. Here are 5 tips that can help you turn flow cytometry into a successful career.

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