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5 Essential Controls For Reproducible Fluorescent Microscopy Imaging

Controls are an integral part of all science. And the complexity of fluorescent microscopy makes including the right controls in your experiments paramount. You should be including these 5 controls in your experiments: an unlabeled sample, a non-specific binding control, a positive and negative control, an antibody titration curve, and blinded image capture. With those controls, you can be sure that your experiments are what you think they are and perform your imaging with confidence. So, happy imaging!

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3 Action Steps You Can Take Right Now To Improve Your Flow Cytometry Reproducibility

Reproducibility is a state of mind. It’s not one simple thing that you do that will make all your data more reproducible, it a shift in the way one thinks about and perform experiments. With the emphasis on rigor and reproducibility in science, it’s very important that researchers start putting into place everything they can do to help improve the quality and reproducibility of there data. Learn 3 action steps that can be taken to enhance experimental reproducibility.

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Microscopy – 5 Reasons Coverslips Are Important For High-Quality Imaging

Most people are familiar with coverslips being placed on slides to protect the sample, but that’s not the only reasons that coverslips are important. They also affect the image quality. Coverslips function by working with your microscope to focus light to a single point and avoiding unnecessary noise in your image. Having the wrong type of coverslip will damage the quality of your images and the quality of the data you extract from those images.

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3 Considerations To Ensure Your Cell Sorting Flow Cytometry Experiments Run Smoothly

There are so many downstream applications of cell sorting, but if you don’t take the time to do you cell sort the right way your downstream experiments won’t work. In order to have the most success with your cell sort be sure you consider these 3 things, size dictates almost everything you are going to do, sample preparation is key, and think about what type of tube you are collecting your cells in. If you account for those 3 things you will set yourself up for a successful cell sort and successful downstream applications.

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3 Questions You Should Be Asking About Flow Cytometry Controls For Your Experiments

Controls are an incredibly important part of your flow cytometry experiments. If not done correctly, poor controls will waste time and money. But with proper care, high-quality controls will result in high-quality data. Just be sure to ask yourself these key questions, should you be using isotype controls, do you have a quality control procedure in place, and are you following the 3 cardinal rules of compensation.

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