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The Right Way To Read A Flow Cytometry Scientific Paper

Reproducibility is a state of mind. It’s not one simple thing that you do that will make all your data more reproducible, it a shift in the way one thinks about and perform experiments. With the emphasis on rigor and reproducibility in science, it’s very important that researchers start putting into place everything they can do to help improve the quality and reproducibility of there data. Learn 3 action steps that can be taken to enhance experimental reproducibility.

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Avoid Data Loss By Following These Steps To Set Your Flow Cytometry Gates Correctly

The way you set your gates in flow cytometry is a key part of your experiment. You need to know that you can find the populations that you’re interested in so you can extract the appropriate data. This is how you can do your secondary or statistical analysis confidently. Learn what gates you should be using and how to define them properly.

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3 Ways To Measure Cell Death With Flow Cytometry

Cell death is a natural part of the lifecycle of a cell. In cases of development, it is critical for the shaping of fingers during human development. The processes of ordered cell death, or Apoptosis, are so important that in 2002, Sidney Brenner, Robert Horvitz, and John Sulston received the Nobel Prize in Medicine for their work on understanding this process. There are many different ways to measure cell death and flow cytometry is an ideal tool for this technique. Whether you are just assessing the viability of your cells or you are interested in the exact stage of cell death your sample is in, there are a variety of ways that you can measure cell death. Learn 3 ways you can use flow cytometry to measure cell death.

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5 Essential Controls For Reproducible Fluorescent Microscopy Imaging

Controls are an integral part of all science. And the complexity of fluorescent microscopy makes including the right controls in your experiments paramount. You should be including these 5 controls in your experiments: an unlabeled sample, a non-specific binding control, a positive and negative control, an antibody titration curve, and blinded image capture. With those controls, you can be sure that your experiments are what you think they are and perform your imaging with confidence. So, happy imaging!

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3 Action Steps You Can Take Right Now To Improve Your Flow Cytometry Reproducibility

Reproducibility is a state of mind. It’s not one simple thing that you do that will make all your data more reproducible, it a shift in the way one thinks about and perform experiments. With the emphasis on rigor and reproducibility in science, it’s very important that researchers start putting into place everything they can do to help improve the quality and reproducibility of there data. Learn 3 action steps that can be taken to enhance experimental reproducibility.

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