What Is A Sample Injection Port

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In flow cytometry, cells, in suspension are moved from the tube to the interrogation point and finally into the waste (or to be sorted, but that is a different story).  To do this, the fluidics components of the flow cytometry are required.

The fluidics are comprised of a running (or Sheath) fluid, that runs through the system in laminar flow.  The movement of this sheath can be achieved by several mechanisms, the most common method using pressure provided by pumps.

The second component of the fluidics is the sample injection port (SIP).  This is where the sample is pushed through to be introduced to the sheath fluid.  Based on the principles of hydrodynamic focusing, these cells are become strung out, single file, in the direction of the flow, where they will pass the interrogation point.

The final main component of the fluidics is the flow cell, which is where everything comes together.

Tim Bushnell

Tim Bushnell

I enjoy answering paradigm-shifting questions and trouble-shooting puzzling glitches. I also like finding new ways to enhance old procedures. I’m passionate about my professional relationships and strive to fill them with positive energy.

My other passions include grilling, wine tasting, and real food. To be honest, my biggest passion is flow cytometry, which is something that Carol and I share. My personal mission is to make flow cytometry education accessible, relevant, and fun. I’ve had a long history in the field starting all the way back in graduate school.
Tim Bushnell
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About Tim Bushnell

I enjoy answering paradigm-shifting questions and trouble-shooting puzzling glitches. I also like finding new ways to enhance old procedures. I’m passionate about my professional relationships and strive to fill them with positive energy. My other passions include grilling, wine tasting, and real food. To be honest, my biggest passion is flow cytometry, which is something that Carol and I share. My personal mission is to make flow cytometry education accessible, relevant, and fun. I’ve had a long history in the field starting all the way back in graduate school.

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