Life Technologies is a company that was acquired by Thermo Fisher Scientific in February 2014. The company offers numerous molecular biology technology products. Antibodies are one of the company’s most popular products. This collection includes primary, secondary, and custom antibodies in addition to antibody purification kits and reagents.
Life Technologies provides numerous primary antibodies that include monoclonal, polyclonal, epigenetic, epitope tag, fusion protein, anti-dye, and cell junction antibodies. The epigenetic antibodies that Life Technologies develops in partnership with the Structural Genomics Consortium (SGC) are the first of their kind. This group of antibodies are developed to allow researchers to determine epigenetic fluctuations in their analysis projects.
To produce their monoclonal antibodies, Life Technologies starts by injecting a host animal with an antigen. This step is completed to start a humoral immune response. The process for generating their polyclonal antibodies is similar. The difference is that the antigen injection is performed to stimulate the B-lymphocytes to generate immunoglobulins (lgG) that are specific to the injected antigen. The mammal’s serum is then used to purify the IgG that is produced.
An anti-dye antibody is a type of primary antibody that Life Technologies offers can serve as substitute signal amplification. These types of antibodies are applicable in research projects that involve flow cytometry, ELISA, western blotting, and DNA hybridization. Because these antibodies have a high specificity, they are suitable for experiments that require signal detection but it is difficult to attain because the background.
The group of antibodies called isotype controls do not have specificity to the target of the experiment, but are comparable to the primary antibody by way of the class and type.
Cancer research is very common in the biomedical community. Life Technologies offers a group of antibodies specifically for cancer research. These antibodies include GTPase, AKT and MAPK signaling pathway, phosphospecific, and transcription-specific antibodies. There are numerous types of cancer cells to study and Life Technologies has attempted to develop antibodies for many of them, including apoptosis, autophagy, tumor suppressors, and metastasis.
Another group of primary antibodies that Life Technologies develops is loading control. These antibodies are developed to assist researchers attain the highest level of efficiency in western blotting. These antibodies are also useful as an additional antibody stain in studies consisting of immunofluorescence. Life Technologies develops loading control antibodies against HRP, beta actin, and a variety of fluorescent dyes.
The ABfinity™ type antibodies are monoclonal antibodies of rabbits that Life Technologies generates from a process that involves the immunizations of animals, functionality screening, and finally making a clone of the immunogen-specific antibody gene that is produced to an expression vector of a mammalian. When this process is done, the antibodies undergo a purification process.
The Life Technologies secondary antibodies serve to target antigens by way of indirect detection. These antibodies are available as conjugated or unconjugated. The main application of these secondary antibodies is to detect primary antibodies for the purpose of western blotting and flow cytometry. The target species that are suitable for these antibodies include horse, guinea pig, dog, cow, chicken, cat, and hamster.
Life Technologies has delivered thousands of custom antibodies. The antibodies are developed in their manufacturing facility, which is certified and registered with applicable federal agencies. While projects vary, custom antibodies generally include monoclonal and polyclonal antibodies, numerous assay options, and antigenic peptide design that is optimized.
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My other passions include grilling, wine tasting, and real food. To be honest, my biggest passion is flow cytometry, which is something that Carol and I share. My personal mission is to make flow cytometry education accessible, relevant, and fun. I’ve had a long history in the field starting all the way back in graduate school.