The Attune® NxT Acoustic Focusing Flow Cytometer is a product that is offered by Life Technologies, a brand that is under the umbrella of Thermo Fisher Scientific. The unique name of this flow cytometer refers to the technology it uses to process cells. Instead of the common method of grouping cells using hydrodynamic forces, the Attune NxT Acoustic Focusing Flow Cytometer is designed to use ultrasonic waves that places the test cells in a single line that appears at the core axis of a capillary.
There are several laser configuration options available with the Attune NxT Flow Cytometer. These configuration options include Blue that has six detection channels and a single Blue laser (488 nm). The Blue/Yellow laser configuration includes a single Blue laser (408 nm) and a single Yellow laser (561 nm). This configuration is able to detect 9 channels. The Blue/Violet laser configuration contains a Violet laser (405 nm) and a Blue laser (488 nm). This combination offer 10 detection channels. Another two/laser configuration is the Blue/Red that includes a Blue laser (488 nm) and a Red laser (637 nm). This configuration supports 9 detection channels. The Blue/Violet/Yellow laser configuration includes 3 lasers, which are the Violet laser (405 nm), Blue laser (488 nm), and Yellow laser (561 nm). This laser configuration has 13 detection channels. The other 3-laser configuration of the Attune NxT Focusing Flow Cytometer is Blue/Red/Violet. This configuration includes Violet laser (405 nm), Blue laser (488 nm), and Red laser (637 nm). The only 4-laser configuration of the instrument is Blue/Red/Yellow/Violet. This configuration includes a Violet laser (405 nm), a Blue laser (488 nm), a Yellow laser (561 nm), and a Red laser (637 nm). This configuration has 16 detection channels. The laser configurations that have fewer than 4 lasers include upgrade options.
Acquiring and analyzing cells with the Attune NxT Focusing Flow Cytometer is performed using the software that is included with the instrument. The software program has a maximum refresh rate of 20-100 million events per sample. The integrated software includes several tools that the operator can use to analyze their cells.
Other features of the Attune NxT Acoustic Focusing Flow Cytometer include automatic cleaning, compatibility with numerous plate formats, and intelligent probe design.
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My other passions include grilling, wine tasting, and real food. To be honest, my biggest passion is flow cytometry, which is something that Carol and I share. My personal mission is to make flow cytometry education accessible, relevant, and fun. I’ve had a long history in the field starting all the way back in graduate school.