The MACSQuant VYB is a flow cytometer that Miltenyi offers as a flexible instrument that is designed as the company’s “yellow laser.” The instrument’s configuration is built on the design of the MACSQuant Analyzer with an optical yellow laser configuration.
The optical configuration of the MACSQuant VYB consists of four lasers: one violet, one blue, and two yellow. The wavelength of the lasers is 405 nm, 488, and 561 nm, respectively. The instrument is capable of detecting up to 10 channels. This instrument has only a limited amount of compensation between FITC and PE, two very common dyes that researchers use in flow cytometry.
The MACSQuant VYB shares a few details with other MACS that Miltenyi develops. In addition to three lasers, the system’s optical system includes forward and side scatter, eight fluorescent channels. It allows researchers to perform walk-away processing of nearly 100 cells simultaneously for one run. This feature requires an optional accessory for the MACSQuant VYB called MACS MiniSampler. This accessory has the capacity to store tube racks of varying formats.
Another common feature that the MACSQuant VYB has with other MACS flow cytometry instruments is that it supports a variety of reagents and kits that can be used to identify cells that are important to study. These include antigen-specific T cells, circulating tumor cells, cancer stem cells, cytokine-secreting cells, endothelial progenitor cells, and dendritic cells.
The MACSQuant VYB also uses absolute cell counts that are identified volumetrically and automated calculated, and delivered in the form of events/uL for each population and sample. Using the MACQuant, researchers are free from having to use costly particles or beads for cell counting.
The MACSQuant VYB is able to identify numerous fluorescent proteins such as YFP and GFP, which are usually hard to differentiate because they have a comparable spectra. The instrument is also optimized to specifically detect PE fluorescence, which is fibroblast marker that is dim.
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My other passions include grilling, wine tasting, and real food. To be honest, my biggest passion is flow cytometry, which is something that Carol and I share. My personal mission is to make flow cytometry education accessible, relevant, and fun. I’ve had a long history in the field starting all the way back in graduate school.