The Guava easyCyte™ 8HT and easyCyte ™10HT are flow cytometers that offers researchers analysis with high throughput (HT). The HT feature of these systems is produced by a robotic sample tray that is able to handle.
The Guava easyCyte 8HT and easyCyte 10HT instruments have some features that are common to all Guava easyCyte instruments, such as microcapillary technology that removes the requirement of adding sheath fluid and allows absolute cell counts without having to reference beads. The systems also have features that are unique in comparison to the other instruments in the easyCyte HT flow cytometer products.
The easyCyte 8HT is a benchtop system that contains dual blue 488 nm and red 640 nm excitation lasers. These lasers offer up to eight detection parameters at one time. The parameters include six fluorescent colors and both forward and side light scatter. This system has a 96-well autosampler built-in that provides researchers plate and tube sampling that is reliable. To acquire and analyze data, the easyCyte 8HT flow cytometer uses InCyte software to acquire and analyze data. In addition to the built-in 96-well plate that stores 10 tubes, this system also has key automation features. The instrument provides automated sampling from the 96 microplates that are part of the system. This feature allows walk-away processing of cell samples. The easyCyte 8HT also includes an automated sampling feature from one or up to 10 tubes of samples. Regular maintenance is also more efficient with the automated cleaning operations.
The easyCyte 10HT is also a benchtop system, but it (as its name indicates) is designed with 10 parameters instead of eight. The parameters provide up to eight fluorescent colors with two scatters of light. The maximum emission include a blue-V with 448/50 nm, Green-V with 525/30 nm, Green-B with 525/30 nm, Red-B with 695/50, Yellow-V with 583/26 nm, and NIR-B with 785/70 nm. The excitation maximum for this instrument is a blue laser with 488 nm and a violet laser with 405 nm. The throughput for this system is 12 uL/min to 60 uL/min.
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My other passions include grilling, wine tasting, and real food. To be honest, my biggest passion is flow cytometry, which is something that Carol and I share. My personal mission is to make flow cytometry education accessible, relevant, and fun. I’ve had a long history in the field starting all the way back in graduate school.