The Guava easyCyte™ 6-2L is a microcapillary flow cytometry system that is developed by EMD Millipore. This benchtop instrument is a two-laser system that consists of six parameters. This instrument is capable of detecting yellow (583/26 nm), green (525/30 nm), and red1 (690/50 nm), and red2 (661/19 nm) light scatters—both forward and side.
The Guava easyCyte products are able to process individual and groups of samples. The compact system includes vials for both washing and waster. The instrument also has a loader for individual samples and a tray for 96-well microplate. The instrument uses a microcapillary fluidics, as well as direct and absolute cell counts.
FlowCellect and Guava kits both support the Guava easyCyte 6-2L system. An EMD MillporeFlowCellect kit allows a researcher to choose whether they want to perform analysis of cell phenotypes or cellular events. Buffer sets, pre-defined gate settings, and protocols are also included in the FlowCellect kits. The Millipore FlowCellect kit is available in four kits (1) The FlowCellectMitoPotential Red Kit, (2) FlowCellectMitoLive Kit, (3) FlowCellectMitoStrees Kit, and (4) FlowCellect Cytochrome c Kit. Each test includes at least 100 tests. Each kit has a set of products that researchers can use for specific projects. For example, the FlowCellectMitoLive Kit includes two dyes that a researcher can use to determine the vitality of mitochondrial cell and health.
Millipore offers several Guava ViaCount kits. Researchers are using the Guava ViaCount more, which is making it customary for cell counting and viability. This assay does not require a wash, which allows researchers to obtain accurate data about cell counts and make assessments related to viability. The kits are called Guava ViaCount Reagent Kit (with either 600 or 100 tests), Guava ViaCount Flex Reagent Kit for challenging samples (total of 100 tests), Guava ViaCount Flex Reagent Kit (total of 500 tests(, and Guava ViaCount Cell Dispersal Reagent, which has 100 tests.
The assays that are common for the easyCyte 6-2L include toxicity screening, cell health, and immunophenotyping.
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My other passions include grilling, wine tasting, and real food. To be honest, my biggest passion is flow cytometry, which is something that Carol and I share. My personal mission is to make flow cytometry education accessible, relevant, and fun. I’ve had a long history in the field starting all the way back in graduate school.