The Guava easyCyte™ 5HT s a single laser-configuration flow cytometer that is manufactured by EMD Millipore. This compact system is part of the Guava easyCyte group of systems.
One of the most significant features of the Guava easyCyte is the microcapillary flow cell system. This patented system is different from the traditional systems that includes a sheath fluid system. This feature enables the Guava easyCyte 5HT to work with an assortment of cells. The flow cell of this instrument aligns itself, and the operator of the system can clean it as needed. The fluidics system also features direct absolute counts that uses a displacement syringe to enable direct sampling.
Another feature of the Guava easyCyte 5HT is sampling for 96-well plates that uses an automated system. This system has a built-in 96-well plate loader tray that the operator can use to store up to 10 tubes of cell samples. A mixing paddle is included with the system to make sure the cells remain in suspension. Researchers have the option to use the guava easyCyte manually to load samples.
The software for the Guava easyCyte 5HT is the data management solution the system uses to acquire and analyze data. The software, called InCyte, includes several features. Operators can allows the system to automatically perform compensation or choose to use the manual process. Gating and analysis tasks in the software are performed with the operator using a drag and drop action. Heat mapping is supported in InCyte as a unique process involving the visualization of a maximum of six parameters at the same time for numerous cell samples.
The Guava easyCyte 5HT system has a single-laser configuration that includes a blue laser with a wavelength of 488 nm. This laser has SSc and FSc that allow it to detect between 3 and 5 parameters in fluorescent channels.
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My other passions include grilling, wine tasting, and real food. To be honest, my biggest passion is flow cytometry, which is something that Carol and I share. My personal mission is to make flow cytometry education accessible, relevant, and fun. I’ve had a long history in the field starting all the way back in graduate school.