The Millipore FlowSight® is an image collection system that generates as many as 12 images of the cells that are being analyzed. This system includes 12 channels that are designed to generate brightfield, a maximum of 10 fluorescence imagery channels, and a darkfield. The range of channel detection coupled with the system’s four excitation enable the FlowSight to measure as many as 10 fluorescent probes at the same time. The 12 channels are a feature that no other compact flow cytometry instrument has. The instrument is also unique in comparison to other instruments in effectiveness that is contributed by its brightfield imagery, a laser committed to side scatter, and laser intensities that can be easily modified.
The visual verification system of the FlowSight uses magnification of -20x, which is the equivalent of a pixel size of 1 micron. This verification system is able to produce fluorescence of cell details, which includes cytoplasm, nucleus, and membrane. The instrument is also able to easily identify single cells from debris and doublets.
Another unique feature of the FlowSight, in comparison to standard flow cytometry instruments, is the process of identifying the cause of cell death. For each and every cell, the FlowSight exposes the nuclear morphology.
The FlowSight has an optional Quantitative Imaging (QI) feature that consists of enhancements of the instrument’s software, computer, and optical system. Together, these enhancements strengthen the capacities of the system’s analysis and imaging. The QI enhancement also includes an automated process for detecting apoptotic cells. The process is in the form of a wizard that checks the nuclear brightfield image contrast and morphology for each cell. The detection process looks at each cell that contains a nuclear stain.
Researchers are also able to expand the capabilities of the FlowSight by choosing one of the modular options. These options include additional (as many as four) excitation lasers and the 96 well autosampler.
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My other passions include grilling, wine tasting, and real food. To be honest, my biggest passion is flow cytometry, which is something that Carol and I share. My personal mission is to make flow cytometry education accessible, relevant, and fun. I’ve had a long history in the field starting all the way back in graduate school.