The JSAN™ (Japan-made sorter, analyzer) is a cell sorter that was developed in the mid-1990s as a desktop system. The JSAN sorter, which is manufactured by Bay Bioscience, includes several features. These features include compact size, 3-laser with 8 color configuration, drop sort, desktop software, and upgrade options.
Cell sorting is the focus of the JSAN. The system contains two patented algorithms, OptiDelay and OptiDrop that are designed to provide high sorting performance. The instrument also includes digital electronics that enable the system to generate droplets at frequencies that are above 60,000.
Several components make up the design of the JSAN, but the instrument’s total size is comparable to an all-in-one system. The compact feature of this system allows the operator to access its controls while seated in the front of it.
The optics design of the JSAN allows researchers to customize it to fit their exact needs. At the very minimum, the system has one 638 nm laser and one 488 nm laser. At the other end of the spectrum, the instrument can be expanded to a configuration with four lasers. The two additional lasers in this configuration are the 405 nm and the 375 nm. With this configuration, the operator can use a maximum of three at the same time.
In addition to customizing laser configuration, operators of the JSAN are able to request custom filters when the optical filters that are part of the system do not meet their needs. JSAN does not guarantee the performance of custom filters that are not tested and recommended by the company.
The control of the JSAN is performed in the instruments Device Parameter control window. The software has a tabbed design that allows users to enter parameters to control the instrument. The tabs include “Instrument Sorting”, “Sort Setting “, and “CloneMate Sorting”. Each tab has a specific function. For example, the “Instrument Setting” tab is where the operator enters parameters to control the system’s optical detection system.
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My other passions include grilling, wine tasting, and real food. To be honest, my biggest passion is flow cytometry, which is something that Carol and I share. My personal mission is to make flow cytometry education accessible, relevant, and fun. I’ve had a long history in the field starting all the way back in graduate school.
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