The S3e™ Cell Sorter is a cell sorter that is developed by Bio-Rad Laboratories Resources, a company that produces a variety of products to assist researchers of life science, biopharmaceutical processing, and clinical diagnostics. The S3e Cell Sorter is considered a compact, benchtop system that uses true jet-in air sorting. The level of purification that the system uses is greater than 99%. The S3e includes several features.
The S3e Cell Sorter includes two unique features. ProDrop™ optimizes the drop delay process. This feature includes automated drop delay calculation and break-off monitoring of droplets. This feature enables the system to perform calculations of drop delay directly by counting bead events.
The AutoGimbal System is another unique feature of the S3e Cell Sorter. The AutoGimbal™ System is an automated system that controls the alignment of the nozzle tip. This feature ensures that the S3e Cell Sorter is consistently aligned on a regular basis.
The system includes 2-way cell sorting, which allows the operator to simultaneously sort two populations of cells that are dissimilar.
The S3e Cell Sorter has a single laser of 488 nm with 100 mW and a dual laser configuration with 488 nm, 561 nm, or 640 nm with 100 mW. The single laser has an upgrade option that extends the laser configuration to 488 nm with 100 mW with 561 nm or 640 nm with 100 mW. This instrument has a maximum of four fluorescence detectors with PMT, forward scatter (FSC) detection with PMT, and side scatter (SSC) detection with PMT.
The fluidics system of the S3e Cell Sorter is onboard and consists of dilution with 8x sheath using deionized water.
There are several accessories available for extending the value of the S3e Cell Sorter. These include water caps, filter blocks, neutral filter blocks, alignment rings, a collection adaptor set, nozzle tip, hex driver, power conditioner, and dichroic filter holder. Users of this cell sorter can purchase the accessory kit, which contains a neutral density filter, spanner wrench, a nozzle tip, syringe, O-ring, and an alignment disk.
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My other passions include grilling, wine tasting, and real food. To be honest, my biggest passion is flow cytometry, which is something that Carol and I share. My personal mission is to make flow cytometry education accessible, relevant, and fun. I’ve had a long history in the field starting all the way back in graduate school.