Since 2002, BioLegend has manufactured reagents and antibodies for use in a variety of biomedical research areas that include cell biology, neuroscience, and immunology. There are several applications for the products that BioLegend develops. Flow cytometry is one of the most common uses for their reagents, but they are also applicable for ELISA, multiplex analysis, functional and enzymatic assays, and immunohistochemistry.
The company’s reagent products are developed in their facility in San Diego, California according to quality standards that meet ISO 9001:2008 and ISO 13485:2003. This certification requires that their manufacturing process is documented, reviewed, and improved on a regular basis.
As part of a goal to extend the capacity of violet lasers and address the needs of multicolor flow cytometry antibody panels, the company partnered with Sirigen to develop the Brilliant Violet ™ family of fluophore conjugated antibodies.
Among the numerous products that BioLegend offers are immunology antibodies, immunoassays, cytokines, chemokines, and cell biology antibodies. The antibody products for immunology are targeted for mouse, human, rat, and non-human primate immunology. Each of these groups is broken down further into other groups. For example, the mouse immunology antibodies include stem cells, dendritic cells, basophils, and neutrophils.
The cell biology category of products that are developed by BioLegend include a variety of antigens, transcriptional regulators, neuronal biomarkers, and angiogenesis. The BioLegend website includes detailed information about each available product. For example, the information about the ABCA3 antibody includes information such as the clone name, the isotype type, reactivity, and proper storage and handling for the antibody. The website also includes antigen details such as a detailed description, other names, and GeneID.
The group of cytokines, chemokines, and factors that BioLegend offers include mouse, human, and rat targets. BioLegend offers other soluble factors such as purified anti-chicken Ovaibumin antibodies.
The BioLegend Immunoassays group of products consists of ELISA kits and sets, mouse ELISA pairs, human ELISA pairs, and flow cytometry kits. The ELISA kits are available in three categories: ELISA MAX™ Standard, ELISA MAX™ Deluxe, and LEGEND MAX™. The ELISA MAX Standard kits and ELISA MAX Deluxe kits are designed for use by ELISA researchers who have at least an intermediate level of experience. The Standard kit is recommended for researchers who already have buffers and plates. However, ELISA coating and wash buffers, assay diluents, and TMB substrate reagent sets are available accessories. Intermediate and experienced researchers who don’t have these items are recommended to use the Deluxe kit because it contains buffers and uncoated plates.
The BioLegend LEGEND MAX kit consists of items for an ELISA research project. This kit consists of reagents and plates that are pre-coated. The LEGEND MAX kit is recommended for researchers who are limited in knowledge and experience of ELISAs. More experienced researchers may also find these kits useful if they need to save time. The BioLegend catalog contains an excess of 80 LEGEND MAX kits.
Each of the kits that BioLegend provides is packaged for specific levels of experience, budgets constraints, and timelines. The flow cytometry kits includes antibody cocktails, human Th17 flow unit, treg flow kits, FOXP3 flow kits, mouse lineage cocktails, human lineage cocktails, and mouse regulatory B cell flow kits.
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My other passions include grilling, wine tasting, and real food. To be honest, my biggest passion is flow cytometry, which is something that Carol and I share. My personal mission is to make flow cytometry education accessible, relevant, and fun. I’ve had a long history in the field starting all the way back in graduate school.