The CytoFLEX by Beckman Coulter is a flow cytometer that is designed with a total of 21 configurations. The system consists of 13 colors and 3 lasers. If an operator opts for the system’s violet laser, they can set the system to calculate side scatter using 405nm for the nanoparticle detection. If necessary, the CytoFLEX is able to provide parameter measurements for light scattering using the 488nm laser. The 405nm laser may also be used in this situation.
The system has a compact design, which allows operators to require only minimal space for placing the system. The instrument is able to house microliter tubes that are 12x75mm. The optical system of the CytoFLEX is based on a design that has a patent pending. The 3 lasers that are included with the system are 488nm, 405nm, and 638nm.
Installation of the CytoFLEX is designed to be easy enough for operators to do it themselves. The design of the system is future proof, which means that researchers can upgrade as needed.
The CytoFLEX includes two reagents, which include the CytoFLEX daily QC fluorospheres and CytoFLEX sheath fluid. A starter kit is part of the delivery package for this instrument to help users become familiar with the system. The system also includes CytExpert, a software application that assists operators of the CytoFLEX with system setup and flow cytometry. In addition to setup, operators can also use CytExpert to set up experiments using the integrated compensation library. The compensation library also stores spillover values of dyes that enable the operator to gauge an adequate amount of compensation. The software is able to track quality-control data results on a regular basis. The software include several templates that operators can use for analyzing their data. The templates include overlay, histogram, contour plot, density plot, dot plot, and pseudo plot.
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My other passions include grilling, wine tasting, and real food. To be honest, my biggest passion is flow cytometry, which is something that Carol and I share. My personal mission is to make flow cytometry education accessible, relevant, and fun. I’ve had a long history in the field starting all the way back in graduate school.