The BD LSR II is a flow cytometer that is offered by BD Biosciences. This instrument has several significant features that include advanced fluidics system, high-performance data acquisition and analysis, and a unique optics system.
The BD LSR II is designed with 4 lasers line colors (488 nm blue, 640 nm red, 405 nm violet, and 355 nm UV). The system is capable of detecting up to 12 parameters at the same time. The laser power for the excitation optics varies. The blue 488 nm laser’s power is 20 mW. A laser power of 40 mW applies to the Red 640 nm laser. The UV 355 nm laser has laser power of 20 mW. Finally, the Violet laser with a wavelength of 405 nm has a laser power of 25 mW.
This product includes three significant features: BD High Throughput Sampler (HTS), optical system based on patented technology, and filters and laser excitation. The integration of a HTS enables the system to process test samples at a rapid rate. Optics are provided by BD Trigon™ and Octagon™. These detector arrays serve to retain laser signals by leading them to their intended detectors.
This product uses BD FACSDiva™ software, which includes tools for performing index sorting and standard flow cytometry tasks such as gating and analysis. This software allows researchers to perform batch or manual analysis and export statistics, FCS files, and experiments in a variety of formats such as CSV, PDF, and XML. The BD FACSDiva software is supported on systems on Windows XP Professional SP3. The minimum processor requirements of this workstation are an Intel® Core™ 2 Due processor, 3.0 GHz.
In addition to cell analysis, the BD LSR II allows researchers to perform multi-color analysis of beads, bacteria, and yeast. DNA analysis is also a supported application of this flow cytometer.
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My other passions include grilling, wine tasting, and real food. To be honest, my biggest passion is flow cytometry, which is something that Carol and I share. My personal mission is to make flow cytometry education accessible, relevant, and fun. I’ve had a long history in the field starting all the way back in graduate school.