The BD LSRFortessa™ is a compact flow cytometry analyzer that is in the suite of flow cytometry instruments that are developed by BD Biosciences. The key features of this system include a fluidics system that uses hydrodynamic focusing, a patented optics system, integrated software for acquisition and analysis, and options to enhance the workflow.
The fluidics system starts with hydrodynamic focusing, which uses pressure to push the cells in a sample to the location where they will be interrogated. The next step of the fluidics design is for the flow cell to become aligned in a fix location with the laser and then gel-coupled to the collection optics. This fluidics system results in the laser being in an optimum position of the sample cells to maximize the total amount of light that is emitted and accumulated to increase the level of sensitivity when the experiment involves multiple colors.
The BD LSRFortessa is designed with an optics system that includes both collection optics and laser excitation. Both of these optics systems are unique to BD Biosciences flow cytometers. The collection optics system includes optical pathways that are shaped like a trigon and octagon. These pathways work to capitalize single detection of the wavelengths that are collected. The excitation optics system includes multiple wavelength lasers, singular pinholes, and beam shaping optics that causes beam spots to separate.
The integration software of BD LSRFortessa, called BD FACSDiva™, is responsible for all the tasks related to the flow cytometry data. This includes all the tasks between setting up the data and performing analysis. The BD FACSDiva software also has an option that allows an operator to work with numerous cell samples. The operator is able to specify the layouts for their experiments.
The BD LSRFortessa offers two options. Researchers can request the BD™ High Throughput Sampler (HTS) to achieve accelerated cell sampling of specific microfilter plates. Researchers are also able to special order products to create a configuration that is ideal for their assay needs.
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My other passions include grilling, wine tasting, and real food. To be honest, my biggest passion is flow cytometry, which is something that Carol and I share. My personal mission is to make flow cytometry education accessible, relevant, and fun. I’ve had a long history in the field starting all the way back in graduate school.