The BD FACSCanto™ II flow cytometer is a benchtop instrument that includes several unique features. This instrument is suitable for studies in cellular metabolism as well as studies in cytotoxicity and DNA analysis. The instrument has an integrated excitation optics system that contains numerous lasers with fixed wavelengths. This feature allows the system to have a fixed alignment that does not require any modification by the user.
The BD FACSCanto system includes two emission signals. The octagon signal, which consists of 5 PMTs and is responsible for detecting light from the Blue laser (488 nm). The system also contains a trigon signal that consists of 2 PMTs that detect Red lasers (633 nm) and Violet lasers (405 nm).
This instrument consists of three lasers that are capable of simultaneously detecting 8 fluorochromes. The blue laser has an excitation of 488 nm, the red laser has an excitation of 633nm, and the violet laser has an excitation of 405 nm. The output of the blue, red, and violet lasers, respectively, is 20mW, 17mW, and 30.
The fluidics system of the BD FACSCanto cytometer is one of its most significant features. To limit the amount of time to start up the system and to enhance reproducibility, the fluidics system uses a fixed-alignment for cell flow. The system works by allowing the cells in the sample to move through an injection tube. Within the tube, a hydrodynamic focusing occurs to push particles of the cells into a stream that is a single line. At this point, light stops the flow of particles at the sample interrogation point.
The B FACSCanto works with the BD FACSDiva software application to control the fluidics system. The software provides several automated systems, such as regular cleaning to prevent buildup of crystals and bacteria.
There are several options available to extend the performance of the BD FACSCanto. These options include the BD FACS™ Loader, BD High Throughput Sampler, and barcode reader.
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My other passions include grilling, wine tasting, and real food. To be honest, my biggest passion is flow cytometry, which is something that Carol and I share. My personal mission is to make flow cytometry education accessible, relevant, and fun. I’ve had a long history in the field starting all the way back in graduate school.