The BD FACSCalibur™ is a flow cytometry instrument that performs operations that are associated with analyzing and sorting cell samples. This benchtop instrument offers researchers numerous features.
The BD FACSCalibur set the standard for performing multi-color analysis using a unique optical system, dual-laser technology, and interbeam compensation. The key to this optical system are two spatially separated lasers that limit the requirement for compensation and allows flexible fluorochrome selection.
The data results that are delivered by the BD FACSCalibur system are provided by BD FACStation™, the automated data management system. This tool includes several features that researchers can use to acquire and analyze data for their projects. These features include statistical analysis, reporting, gating, and plot creation.
The BD FACSCalibur system also includes the BD FACSComp™ software application and BD Calibrite™ beads that work together to deliver the instrument’s quality assurance system. These two products are responsible for setting the compensation for immunofluorescence applications, setting up the BD FACSCalibur, and determine the level of sensitivity.
Researchers are able to expand the performance and capabilities of the BD FACSCalibur by using one or more of the options that are available. The BD™ High Throughput Sampler (HTS) is an optional addition to the BD FACSCalibur that is able to automatically acquire samples from 96- and 384-well microtiter plates. When the system is set to high-throughput mode, the BD HTS can process a 96-well plate in a few minutes with only a limited amount of sample carryover that can occur among the wells.
Another option for the BD FACSCalibur is the BD FACS™Loader that is an automated system for applications researchers need to perform a regular basis. This system includes a removable cartridge that can hold 40 tubes.
A barcode reader can also work with the BD FACSCalibur system for generating barcode labels that are one-dimensional.
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My other passions include grilling, wine tasting, and real food. To be honest, my biggest passion is flow cytometry, which is something that Carol and I share. My personal mission is to make flow cytometry education accessible, relevant, and fun. I’ve had a long history in the field starting all the way back in graduate school.
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