The BD Accuri™ C6 is a compact cell analyzer that is developed by BD Biosciences. The primary features of this instrument include pre-optimized scatter detectors, flexible options, intuitive software, and customization.
The optics system of the BD Accuri C6 includes two light scatter detectors, one red laser and a single blue laser, and four fluorescence detectors. The optical filters that are associated with the fluorescence detectors are able to identify PerCP, PE, FITC, and APC. The system is also capable of performing most apoptosis assays, including caspase activation, mitochondrial membrane potential, and PARP cleavage. When a BD Accuri C6 is manufactured, the elements of its optical system are secured so that the voltages of the detectors does not require adjustment by the system operators. If researchers need to expand the configuration of the system, they can use filters and the Selectable Laser Module that are able to enhance the combination of fluorochromes. This requires a simple setting modification, and then the operator can analyze the data again.
The fluidics system of the BD Accuri C6 uses a pumping system that is specific to instruments developed by BD Biosciences. The system has a core that is sheath-focused, which is able to produce event rates that top 10,000 events per second. The fluidics system also allows the instrument to generate sample concentration that is greater than 5x106 cells per milliliter. Operators of the BD Accuri C6 are able to use any brand of sample tubes, as long as they measure no greater than 12x75 mm.
One of the most significant flexible options of the BD Accuri is walkway sample loading. This feature of the instrument allows an operator to setup an experiment and use BD CSampler™, an optional component that place the system in automation mode.
The software that the BD Accuri 6 uses for data acquisition and analysis is designed to accommodate researchers who have limited flow cytometry experience. The user interface of the software is designed with tabs that cover the main tasks that a researcher will perform with the software. The software provides sample data that the operator can customize according to the requirements of their projects. If an operator needs to pull-in a plot that was developed on another compatible system, they can do so using the import feature. Other features of the software include event coloring, live gating, vector-scalable graphics, and batch analysis.
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My other passions include grilling, wine tasting, and real food. To be honest, my biggest passion is flow cytometry, which is something that Carol and I share. My personal mission is to make flow cytometry education accessible, relevant, and fun. I’ve had a long history in the field starting all the way back in graduate school.