The ImageStream® x Mark II Imaging Flow Cytometer is an instrument that includes CCD camera in time-delay integration, extended depth of field, and multispectral imaging. The CCD camera functions with time delay integration (TDI) that is a mode that uses an electrical system to track the movements of objects and then moves the pixel count of those objects one row at a time. When the system reaches the last row, it then collects the aggregated pixel content.
The multispectral imaging system within the ImageStream Mark II Flow Cytometer enables the system to achieve an acquisition rate of 12 images/cell using three unique modes of imaging. The modes are fluorescence, brightfield, and darkfield.
The extended depth of field (EDF™) feature of the ImageStream Mark II Flow Cytometer is a non-standard optional system that extends cell depth without losing any fluorescence sensitivity. This feature is combined with Wavefront Coding™ technology that stems from CDM Optics. This refers to an algorithm for processing images. Together, these two systems enable the system to display an extended number of structures inside the cell into a single line of focus. By extending the depth of field, the EDF feature enables the system to be upgraded with ease as new application become available.
There are numerous flow cytometry applications that are supported by the ImageStream Mark II Imaging Flow Cytometer. These applications include morphology, internalization, DNA damage and repair, cell death, microbiology, oncology, cell-cell interactions, co-localization, targeted immunotherapy, stem cell differentiation, autophagy, cell cycle and mitosis, and parasitology. For each of these applications, the ImageStream Mark II Imaging Flow Cytometer has a unique offering. For example, in relation to morphology the instrument includes an image analysis software application called IDEAS that uses pre-defined settings to measure cell shape. The software application also allows the system to detect cells that experiencing autophagy.
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My other passions include grilling, wine tasting, and real food. To be honest, my biggest passion is flow cytometry, which is something that Carol and I share. My personal mission is to make flow cytometry education accessible, relevant, and fun. I’ve had a long history in the field starting all the way back in graduate school.