Dako was founded in 1966 by Niels HarboeDako, a Danish medical doctor. In 2012, the company was acquired by Agilent Technologies. Dako, now an Agilent Technologies company, is a provider of a variety of products that support biomedical analysis projects. Their offerings include reagents, antibodies, instruments, and software. In addition to instruments Dako develops products that belong to the following areas: flow cytometry, molecular pathology, pharmacoDiagnostic®, and immunohistochemistry (IHC).
The Dako flow cytometry products include primary single-color reagents, dual color reagents, isotype and control reagents, MultiMix™ Triple Color Reagents, secondary antibody conjugates, and streptavidin conjugate, and kits. The primary single-color reagents have a single flurochrome that they are conjugated with for researchers to use in flow cytometry research projects. Gel filtration causes the unreacted fluorochromes to be removed after the antibodies are conjugated. Dako offers several flurochromes that includes Pacific Blue (PB), Cascade Yellow (CY), Allophycocyanin (APC), Fluorescein (FITC), Peridinin Chlorophyll Protein (PerCP), Phycoerythrin (RPE), and Phycoerythrin-Cy5 (RPE-Cy5).
The dual-color reagents that are generated by Dako consist of a combination of multiple antibodies that are labeled with the FITC and RPE conjugates. There are several dual-color reagents that include CD45/FITC plus CD14/RPE and CD3/FITC plus CD19/RPE.
The Dako flow cytometry products include mouse and rabbit antibody controls. The mouse antibody controls that Dako produces are established using mouse antibodies from other isotypes, and then used for the Aspergillus niger glucose oxidase. Researchers who select these isotypes receive conjugated antibodies that are also purified. The rabbit antibody controls are derived from the serum of rabbits that have not been immunized. The rabbit antibody controls follow the same process as F(ab’)2 fragment rabbit antibodies that are solid-phase absorbed.
The MultiMix Triple-Color Reagents that Dako develops consists of a total of three antibodies that have the labels of FITC, APC, RPE, and RPE-Cy5. The fluorochromes consists of specific dyes. The RPE-Cy5 fluorochromes are combined with cyanine dye. The Cy5 dye is coupled with the RPE fluorochromes.
Be the first to leave a review.
My other passions include grilling, wine tasting, and real food. To be honest, my biggest passion is flow cytometry, which is something that Carol and I share. My personal mission is to make flow cytometry education accessible, relevant, and fun. I’ve had a long history in the field starting all the way back in graduate school.
Latest posts by Tim Bushnell (see all)
- We Tested 5 Major Flow Cytometry SPADE Programs for Speed – Here Are The Results - December 5, 2019
- Mass Cytometry Revolves Around These 5 Things - November 21, 2019
- Understanding Reproducibility in Flow Cytometry – It’s the Antibodies! - October 24, 2019