The BD FACSAria™ Fusion is a flow cytometer that provides cell sorting. The instrument performs the tasks required for flow cytometry using BD FACSDiva™ software. The software provides the setup, acquirement, and analysis tasks with only minimal intervention required from the operator. This software is compatible with common BD cell sorters and analyzers. This enables researchers to utilize other systems for specific tasks without having to worry about incompatibility.
In addition to analysis and acquisition using the BD FACSDiva software, the BD FACSAria Fusion offers other significant features that include index sorting, digital electronics, and acquisition and analysis. The index sorting feature allows the operator to view the entire phenotype of the cell surface into a soft device that contains multiple positions. During each event in the cell sorting process, the operator is able to obtain information about
Beckman Coulter CytoFlex
The CytoFLEX by Beckman Coulter is a flow cytometer that is designed with a total of 21 configurations. The system consists of 13 colors and 3 lasers. If an operator opts for the system’s violet laser, they can set the system to calculate side scatter using 405nm for the nanoparticle detection. If necessary, the CytoFLEX is able to provide parameter measurements for light scattering using the 488nm laser. The 405nm laser may also be used in this situation.
The system has a compact design, which allows operators to require only minimal space for placing the system. The instrument is able to house microliter tubes that are 12x75mm. The optical system of the CytoFLEX is based on a design that has a patent pending. The 3 lasers that are included with the system are 488nm, 405nm, and 638nm.
Installation of the CytoFLEX is designed to be easy enough for operators to do it themselves. The design of
Beckman Coulter Astrios
The MoFlow® Astrios instrument by Beckman Coulter is a combination of the original MoFlow and the MoFlow XDP. This combination of instruments results in a machine that contains a wide selection of lasers and capabilities for cell sorting.
The MoFlowAstrios is designed with several new features. The auto start up feature allows the operator to determine the settings for auto start of the fluidics and lasers. The system has a touch screen. The auto QC feature of the MoFlowAstrios allows researchers to track performance for more than 30 parameters. IntelliSort is a feature for monitoring and drop determination that is bead-free.
There are several optics available with the MoFlowAstrios instrument that includes 7 lasers for two of the alignment stages, single mutli-fiber Beam Shaping Optic (BSO), and a flat-top beam profile that is on coupled lasers.
This version of the MoFlowAstrios system includes Summi
The NovoCyte is a flow cytometer is developed by ACEA Biosciences, Inc. One of the main features of the system is that it is customizable. The operator has the option to choose to use 1 or up to 3 lasers. As necessary, operators can also exchange filters and use numerous analysis formats and sampling. The system is able to detect up to 15 parameters.
Another significant feature of the NovoCyte is the volumetric fluidics system that performs counting that is direct absolute. This is contrary to a common method that uses reference beads that can be expensive. The system’s has pressure sensors that are able to monitor the status of fluidics in real-time, which averts an obstruction of the flow path. This also enables the system to provide data that is more accurate and reliable.
The NovoCyte has an optical system that uses self-regulating beam shaping. This optical system, which has a patent pending, also has a
The Millipore FlowSight® is an image collection system that generates as many as 12 images of the cells that are being analyzed. This system includes 12 channels that are designed to generate brightfield, a maximum of 10 fluorescence imagery channels, and a darkfield. The range of channel detection coupled with the system’s four excitation enable the FlowSight to measure as many as 10 fluorescent probes at the same time. The 12 channels are a feature that no other compact flow cytometry instrument has. The instrument is also unique in comparison to other instruments in effectiveness that is contributed by its brightfield imagery, a laser committed to side scatter, and laser intensities that can be easily modified.
The visual verification system of the FlowSight uses magnification of -20x, which is the equivalent of a pixel size of 1 micron. This verification system is able to produce fluorescence of cell
Thermo Fisher Attune NxT
The Attune® NxT Acoustic Focusing Flow Cytometer is a product that is offered by Life Technologies, a brand that is under the umbrella of Thermo Fisher Scientific. The unique name of this flow cytometer refers to the technology it uses to process cells. Instead of the common method of grouping cells using hydrodynamic forces, the Attune NxT Acoustic Focusing Flow Cytometer is designed to use ultrasonic waves that places the test cells in a single line that appears at the core axis of a capillary.
There are several laser configuration options available with the Attune NxT Flow Cytometer. These configuration options include Blue that has six detection channels and a single Blue laser (488 nm). The Blue/Yellow laser configuration includes a single Blue laser (408 nm) and a single Yellow laser (561 nm). This configuration is able to detect 9 channels. The Blue/Violet laser configuration contains a Violet la
The S3e™ Cell Sorter is a cell sorter that is developed by Bio-Rad Laboratories Resources, a company that produces a variety of products to assist researchers of life science, biopharmaceutical processing, and clinical diagnostics. The S3e Cell Sorter is considered a compact, benchtop system that uses true jet-in air sorting. The level of purification that the system uses is greater than 99%. The S3e includes several features.
The S3e Cell Sorter includes two unique features. ProDrop™ optimizes the drop delay process. This feature includes automated drop delay calculation and break-off monitoring of droplets. This feature enables the system to perform calculations of drop delay directly by counting bead events.
The AutoGimbal System is another unique feature of the S3e Cell Sorter. The AutoGimbal™ System is an automated system that controls the alignment of the nozzle tip. This feature ensures that the S3e Ce
Beckman Coulter Gallios
Beckman Coulter offers several flow cytometers. The Gallios™ is a system is designed to acquire as many as 10 colors. The system also includes an optical system that enables it to handle multicolor assays. There are several other features of the Gallios instrument.
The Gallios offers four laser configurations. The six-color configuration includes 2 lasers consisting of Blue 488 nm and Red 638 nm). The eight-color configuration consists of 2 lasers that are Blue 488 and Red 638. The 10-color configuration includes 3 lasers of Blue 488 nm, Red 638 nm, and Violet 405 nm). The system includes another 10-color configuration with 3 lasers. This configuration includes Blue 488 nm, Yellow 561 nm, Red 638 nm, and Violet 405 nm. The Yellow laser is co-linear.
The scatter detection feature of the Gallios system is unique. The forward-scatter detector is able to perform up to three cell-size measurements at a v
The ImageStream® x Mark II Imaging Flow Cytometer is an instrument that includes CCD camera in time-delay integration, extended depth of field, and multispectral imaging. The CCD camera functions with time delay integration (TDI) that is a mode that uses an electrical system to track the movements of objects and then moves the pixel count of those objects one row at a time. When the system reaches the last row, it then collects the aggregated pixel content.
The multispectral imaging system within the ImageStream Mark II Flow Cytometer enables the system to achieve an acquisition rate of 12 images/cell using three unique modes of imaging. The modes are fluorescence, brightfield, and darkfield.
The extended depth of field (EDF™) feature of the ImageStream Mark II Flow Cytometer is a non-standard optional system that extends cell depth without losing any fluorescence sensitivity. This feature is combined with
The Muse® Cell Analyzer is a compact cytometry instrument that is designed to allow biomedical researchers to perform cell sample setup and analysis in a few steps. The Muse instrument provides a wide variety of cell analysis miniaturized optics and by using microcapillary technology. These features of the system take up less space. In contrast to instruments that are based on images, the Muse uses a laser-based system to detect fluorescence.
The design of the Muse Cell Analyzer is intended to provide greater use of workspace and to simplify the flow cytometry process. The system uses a 5-step process to analyze cell. The steps are (1) prepare the sample, (2) load the sample, (3) modify settings for the specific application, (4) obtain data, and (5) analyze the data. The instrument has a 7-inch touchscreen display that contains all the system controls. Located just outside the display is a loading arm that is low
The BD FACSCanto™ II flow cytometer is a benchtop instrument that includes several unique features. This instrument is suitable for studies in cellular metabolism as well as studies in cytotoxicity and DNA analysis. The instrument has an integrated excitation optics system that contains numerous lasers with fixed wavelengths. This feature allows the system to have a fixed alignment that does not require any modification by the user.
The BD FACSCanto system includes two emission signals. The octagon signal, which consists of 5 PMTs and is responsible for detecting light from the Blue laser (488 nm). The system also contains a trigon signal that consists of 2 PMTs that detect Red lasers (633 nm) and Violet lasers (405 nm).
This instrument consists of three lasers that are capable of simultaneously detecting 8 fluorochromes. The blue laser has an excitation of 488 nm, the red laser has an excitation of 633nm, and the
The JSAN™ (Japan-made sorter, analyzer) is a cell sorter that was developed in the mid-1990s as a desktop system. The JSAN sorter, which is manufactured by Bay Bioscience, includes several features. These features include compact size, 3-laser with 8 color configuration, drop sort, desktop software, and upgrade options.
Cell sorting is the focus of the JSAN. The system contains two patented algorithms, OptiDelay and OptiDrop that are designed to provide high sorting performance. The instrument also includes digital electronics that enable the system to generate droplets at frequencies that are above 60,000.
Several components make up the design of the JSAN, but the instrument’s total size is comparable to an all-in-one system. The compact feature of this system allows the operator to access its controls while seated in the front of it.
The optics design of the JSAN allows researchers to customize it to fit their
Millipore/Guava easyCyte 8-10HT
The Guava easyCyte™ 8HT and easyCyte ™10HT are flow cytometers that offers researchers analysis with high throughput (HT). The HT feature of these systems is produced by a robotic sample tray that is able to handle.
The Guava easyCyte 8HT and easyCyte 10HT instruments have some features that are common to all Guava easyCyte instruments, such as microcapillary technology that removes the requirement of adding sheath fluid and allows absolute cell counts without having to reference beads. The systems also have features that are unique in comparison to the other instruments in the easyCyte HT flow cytometer products.
The easyCyte 8HT is a benchtop system that contains dual blue 488 nm and red 640 nm excitation lasers. These lasers offer up to eight detection parameters at one time. The parameters include six fluorescent colors and both forward and side light scatter. This system has a 96-well aut
The BD LSRFortessa™ is a compact flow cytometry analyzer that is in the suite of flow cytometry instruments that are developed by BD Biosciences. The key features of this system include a fluidics system that uses hydrodynamic focusing, a patented optics system, integrated software for acquisition and analysis, and options to enhance the workflow.
The fluidics system starts with hydrodynamic focusing, which uses pressure to push the cells in a sample to the location where they will be interrogated. The next step of the fluidics design is for the flow cell to become aligned in a fix location with the laser and then gel-coupled to the collection optics. This fluidics system results in the laser being in an optimum position of the sample cells to maximize the total amount of light that is emitted and accumulated to increase the level of sensitivity when the experiment involves multiple colors.
The BD LSRFortessa
The BD LSRFortessa™ X20 is a cell analyzer that has the capability to detect as many as 18 colors at the same time while supporting 5 lasers. The instrument is designed as a desktop system that requires minimal space, but with performance that at least equals that of larger systems. There are customizable options for the number of wavelengths (up to 16) and laser power.
The BD LSRFortessa X20 has a compact size that allows it to fit into most work space areas. Its design also features components that are easily accessed by the instrument’s operator.
Following the design of other instruments in its class that are designed by BD Biosciences, the BD LSRFortessa contains an optics system that includes both excitation and collection optics. The excitation optics system includes numerous lasers with a fixed wavelength, beam-shaping optics, and singular pinholes that cause beam spots to become spatially separated. The col
BD LSR II
The BD LSR II is a flow cytometer that is offered by BD Biosciences. This instrument has several significant features that include advanced fluidics system, high-performance data acquisition and analysis, and a unique optics system.
The BD LSR II is designed with 4 lasers line colors (488 nm blue, 640 nm red, 405 nm violet, and 355 nm UV). The system is capable of detecting up to 12 parameters at the same time. The laser power for the excitation optics varies. The blue 488 nm laser’s power is 20 mW. A laser power of 40 mW applies to the Red 640 nm laser. The UV 355 nm laser has laser power of 20 mW. Finally, the Violet laser with a wavelength of 405 nm has a laser power of 25 mW.
This product includes three significant features: BD High Throughput Sampler (HTS), optical system based on patented technology, and filters and laser excitation. The integration of a HTS enables the system to process test samples at a r
Millipore/Guava easyCyte 5HT
The Guava easyCyte™ 5HT s a single laser-configuration flow cytometer that is manufactured by EMD Millipore. This compact system is part of the Guava easyCyte group of systems.
One of the most significant features of the Guava easyCyte is the microcapillary flow cell system. This patented system is different from the traditional systems that includes a sheath fluid system. This feature enables the Guava easyCyte 5HT to work with an assortment of cells. The flow cell of this instrument aligns itself, and the operator of the system can clean it as needed. The fluidics system also features direct absolute counts that uses a displacement syringe to enable direct sampling.
Another feature of the Guava easyCyte 5HT is sampling for 96-well plates that uses an automated system. This system has a built-in 96-well plate loader tray that the operator can use to store up to 10 tubes of cell samples. A mixing pad
The MACSQuant® Analyzer 10 is a cell analyzer that is developed by MiltenylBiotec to perform automated multi-color flow cytometry. This instrument is a benchtop flow cytometer that is designed to occupy a minimal amount of space. The highlights of this machine include its optical system, multi-sample capability, and aut nm, omated system for labeling MACSQuant column that is used for magnetic rare cell pre-enrichment.
The MACSQuant Analyzer 10 consists of three lasers that have wavelengths of 405 nm, 638 nm, and 488 nm. The system also uses front and side scatter parameters to determine the relative size of samples. There are also eight fluorescent channels that are available. In a single run this instrument is able to process 96 cell samples when it is optimized with the MACS MiniSampler, an optional accessory. The MACS MiniSampler can hold numerous tube racks of varying formats in addition to miccrotiter
The MACSQuant VYB is a flow cytometer that Miltenyi offers as a flexible instrument that is designed as the company’s “yellow laser.” The instrument’s configuration is built on the design of the MACSQuant Analyzer with an optical yellow laser configuration.
The optical configuration of the MACSQuant VYB consists of four lasers: one violet, one blue, and two yellow. The wavelength of the lasers is 405 nm, 488, and 561 nm, respectively. The instrument is capable of detecting up to 10 channels. This instrument has only a limited amount of compensation between FITC and PE, two very common dyes that researchers use in flow cytometry.
The MACSQuant VYB shares a few details with other MACS that Miltenyi develops. In addition to three lasers, the system’s optical system includes forward and side scatter, eight fluorescent channels. It allows researchers to perform walk-away processing of nearly 100 cells
The BD FACSCalibur™ is a flow cytometry instrument that performs operations that are associated with analyzing and sorting cell samples. This benchtop instrument offers researchers numerous features.
The BD FACSCalibur set the standard for performing multi-color analysis using a unique optical system, dual-laser technology, and interbeam compensation. The key to this optical system are two spatially separated lasers that limit the requirement for compensation and allows flexible fluorochrome selection.
The data results that are delivered by the BD FACSCalibur system are provided by BD FACStation™, the automated data management system. This tool includes several features that researchers can use to acquire and analyze data for their projects. These features include statistical analysis, reporting, gating, and plot creation.
The BD FACSCalibur system also includes the BD FACSComp™ software application and BD C
BD Aria III
A cell sorter in the BD Biosciences suite of instruments is the BD FACSAria™ III. This instrument is a cell sorter that is designed with high-performance cell sorting for a wide range of applications. The sorting capabilities of this instrument are tied to its design, which involves using gel-coupled flow cell and a fixed optical system. This design is very similar to BD Biosciences analyzers, such as BD LSRFortessa™ and BD FACSCanto™ II. The design of the flow cell coupled with the design of the nozzle allows the system to obtain a significant collection of light, which in turn speeds up the rate that the particle is able to pass through the nozzle.
In addition to its cell sorting capabilities, the BD FACSAria also includes sensitivity for multicolor and sorting applications, an optics system that provides multicolor detection, and software for data acquisition and analysis. The optics system that the BD fAC
The BD Accuri™ C6 is a compact cell analyzer that is developed by BD Biosciences. The primary features of this instrument include pre-optimized scatter detectors, flexible options, intuitive software, and customization.
The optics system of the BD Accuri C6 includes two light scatter detectors, one red laser and a single blue laser, and four fluorescence detectors. The optical filters that are associated with the fluorescence detectors are able to identify PerCP, PE, FITC, and APC. The system is also capable of performing most apoptosis assays, including caspase activation, mitochondrial membrane potential, and PARP cleavage. When a BD Accuri C6 is manufactured, the elements of its optical system are secured so that the voltages of the detectors does not require adjustment by the system operators. If researchers need to expand the configuration of the system, they can use filters and the Selectable Laser Module that
Millipore/Guava easyCyte 6-2L
The Guava easyCyte™ 6-2L is a microcapillary flow cytometry system that is developed by EMD Millipore. This benchtop instrument is a two-laser system that consists of six parameters. This instrument is capable of detecting yellow (583/26 nm), green (525/30 nm), and red1 (690/50 nm), and red2 (661/19 nm) light scatters—both forward and side.
The Guava easyCyte products are able to process individual and groups of samples. The compact system includes vials for both washing and waster. The instrument also has a loader for individual samples and a tray for 96-well microplate. The instrument uses a microcapillary fluidics, as well as direct and absolute cell counts.
FlowCellect and Guava kits both support the Guava easyCyte 6-2L system. An EMD MillporeFlowCellect kit allows a researcher to choose whether they want to perform analysis of cell phenotypes or cellular events. Buffer sets, pre-defined gate s
Millipore/Guava easyCyte 5 HPL
The Guava easyCyte™ 5 HPL is in a group of benchtop flow cytometers that are developed by EMD Millipore. These compact systems require minimal space and offer researchers several features for completing their flow cytometry projects.
Some features and benefits of the Guava easyCyte 5 HPL are standard with easyCtye instruments. The fluidics system is based on a patented microcapillary design that differs from the use of a sheath-fluid design that is standard for most flow cytometers. In the microcapilliary design, the instrument uses smaller volumes of the samples, produces a limited amount of waste material, and requires less to operate. The waste generation is generally less than 40 milliliters after eight hours of continuous use. As standard with Guava easyCyte systems, the Guava easyCyte 5 HPL uses digital signal processing. The sample format is either an automated 96-well plate loader or a sing
Millipore/Guava easyCyte 8-10
The Guava easyCyte™ 8 and Guava easyCyte 10 are instruments by EMD Millipore that are designed to be used in flow cytometry projects.
There are several features that are standard with EMD Millipore’s suite of easyCyte instruments. These features include a microcapillary fluidics system, direct absolute cell counts, digital signal processing and data acquisition and analysis using the InCyte™ software application. Microcapillary flow cytometry refers to a patented fluidics system that features a flow cell that provides absolute cell counts. This fluidics system also negates the need for sheath fluid.
The InCyte software application allows researchers to concentrate on data that is experimental or an actual sample. The software application uses automation for compensation and provides other features such as plot analysis, setup, and drag and drop. The software allows researchers to perform analy
FCS Express is a suite of flow cytometry analysis tools developed by De Novo Software to enable biological research professionals to generate rapid data results in various custom outputs layouts.
The data is viewable onscreen, printed, or exported to a Microsoft Excel file. The FCS Express tools provide? The interface is designed to mimic the ribbon and tab system features that are found in the widely used Microsoft Word™ application.
The FCS Express application includes several features such as histograms, gates, 2D plots, and statistics.
FCS Express includes a histogram feature that allows the user to change the parameter in view by using a single click. As an alternative, the user can simply select the number of the parameter they prefer to view. If the user needs to zoom in closer to an area of a histogram, they can do so by using a click and drag movement with the mouse on the area they want to see closer.
The FlowJo flow cytometry software application allows researchers to analyze the entirety of their experiments, even if there are numerous samples. The software application is developed with an environment that integrates the features, which allows users to view and analyze their data in a single location. One area of the application is called the workspace. This section consists of samples that are loaded, as well as gates and statistics. All of the information that is on the workspace is saved by the user in the form of a FlowJo document on the local drive.
Common features such as statistics and gates are included with the FlowJo software application. There are unique features such as an analysis platform that allows the user to process assays that involve proliferation, kinetics, and DNA/cell cycle.
Users are able to use the FlowJo software application to produce data results in numerous formats that include reports,
The Kaluza flow analysis software application is produced by Beckman Coulter. The focus of the application is users who produce large quantities of data. Kaluza is a software solution that is capable of performing real-time flow cytometry analysis of up to 10 million cell events.
There are several usability features in Kaluza that allow the user to do more data analyzing than clicking their mouse. Kaluza includes a radial menu that lessens the number of interactions that users have with the mouse. The application includes a workspace that keeps the amount of items on the desktop to a minimum. Users are able to load multiple data files and process their data in a batch. If users need to copy existing plots, they can do so using the copy and paste feature to select multiple plots.
Users have several options for plots. They can chose a tree plot to categorize cells. If they want to see results in 3D, they can use the radar
Winlist™ is a flow cytometry software application developed by Verity Software House. The application is able to read flow cytometry software (FCS) files. It also includes region tools. Users are able to create overlays and reports that automatically update. The overlays are available for both 1P and 2P plots.
Winlist supports manual compensation using N-Color Compensation™. This manual method involves the system using the default compensation setting and allowing the researcher to make adjustments as necessary. The software also includes V-Comp™, which is a tool that automates the data compensation process. This tool works by automatically compensating the control files that you specify. The software doesn’t have a limit on the number of fluors that you can specify.
Batch processing in the Winlist software package is done using one of two options. One is a two-step process that involves selecting the files and c
FlowLogic is one of three software applications that is developed by Inivai Technologies. The FlowLogic platform consists of four software products that provide specific functions in the flow analysis process: gatelogic™, platelogic™, graphiclogic™, and doclogic™ . The benefit of having all products together is to create a continuous flow between the different operations. FlowLogic also allows users to perform their analysis more efficiently since they are using compatible tools.
The gatelogic product is a research tool for performing standard flow cytometry analysis. The user interface for this software consists of a toolbar, workspace area, advance function panel, navigator panel, and file inspector. Of these, the workspace and advanced function panel are the most significant areas. Researchers open FCS files in this area and are able to perform several analysis processes. The advanced function panel is wher
Since 2002, BioLegend has manufactured reagents and antibodies for use in a variety of biomedical research areas that include cell biology, neuroscience, and immunology. There are several applications for the products that BioLegend develops. Flow cytometry is one of the most common uses for their reagents, but they are also applicable for ELISA, multiplex analysis, functional and enzymatic assays, and immunohistochemistry.
The company’s reagent products are developed in their facility in San Diego, California according to quality standards that meet ISO 9001:2008 and ISO 13485:2003. This certification requires that their manufacturing process is documented, reviewed, and improved on a regular basis.
As part of a goal to extend the capacity of violet lasers and address the needs of multicolor flow cytometry antibody panels, the company partnered with Sirigen to develop the Brilliant Violet ™ family of fluophore
Militenyl Biotec is a developer and manufacturer of products to support individuals that work in the biomedical research industry. The company provides reagents in the form of single antibodies or kits with a combination of antibodies. The antibodies that are available are suitable for a variety of cell targets such as human, non-human primate, and mouse.
The Reafinity™ Antibodies go through a unique engineering process that negates them having .to go through fragment crystallization or binding. This process also provides the antibodies with the IgG1 human isotype that lessens the number of negative controls that are required to one. They also consist of a single heavy and light chain. These antibodies, which are recombinant, are available for numerous specificities. Reafinity antibodies are developed in mammalian cells and have 300 clones. CD5, CD10, IgD, and Ki-67 are examples of the antibodies that are develope
The AbD Serotec company produces antibodies for researchers in the scientific community. The company’s featured products include C4d, CD206, and F4/80. The C4d antibody was developed to detect the 4d component, which is made of three pathways that serve to assist the immune system in combating infection by clearing pathogens. The CD206 antibody is associated with the mannose receptor group of receptors. This molecule is available in a variety of cell types such as retinal pigment epithelium. The F4/80 antibody, which is associated with the EGF-TM7 group, is a marker for mouse macrophage. The AbD Serotec suite of F4/80 products are available in a variety of formats (includes APC, FITC, RPE) and applications. The most common applications are flow cytometry and immunofluorescence.
The company also produces cryptosporidium antibodies. This includes nine rituximab antibodies that are suitable for nonclinical studies
Thermo Fisher/Life Technologies
Life Technologies is a company that was acquired by Thermo Fisher Scientific in February 2014. The company offers numerous molecular biology technology products. Antibodies are one of the company’s most popular products. This collection includes primary, secondary, and custom antibodies in addition to antibody purification kits and reagents.
Life Technologies provides numerous primary antibodies that include monoclonal, polyclonal, epigenetic, epitope tag, fusion protein, anti-dye, and cell junction antibodies. The epigenetic antibodies that Life Technologies develops in partnership with the Structural Genomics Consortium (SGC) are the first of their kind. This group of antibodies are developed to allow researchers to determine epigenetic fluctuations in their analysis projects.
To produce their monoclonal antibodies, Life Technologies starts by injecting a host animal with an antigen. This ste
BD is a worldwide company that provides medical technology products for a variety of industries. Of their worldwide segments, BD Biosciences is the segment of the company that has a focus on delivering cellular analysis products and services to support researchers in life sciences. The BD Biosciences products and services include monoclonal antibodies and kits, fluorescence-activated cell sorters and analyzers, reagent systems, cell imaging systems, and additional cell culture media products.
BD Biosciences provides a variety of products and services for the flow cytometry industry. These include instruments, applications, cell culture, and reagents. The reagents that BD Biosciences develops are single antibodies, ASR reagents, and multicolor cocktails.
BD Biosciences offers several reagent products in the form of antibodies and buffers, immunoassays, cell-based assays. The antibodies products include primary
The eBioscience company has been in existence since the late 1990s. Since that time, the company has eBioscience develops antibody products for a variety of uses that include flow cytometry, gene expression, ELISA, and clinical. The company’s clinical diagnostic reagents and analyte specific reagents (ADR) are enhanced specifically for use in clinical laboratories. This category of products have expanded as a result of a partnership that eBioscience has entered into with Binding Site, a company that specializes in the management and diagnosis of blood cancer. These reagents support a test size up to 120 and a 5 uL/ concentrated formula. The fluorochromes that are available are suitable or most specificities. They are also compatible with the flow cytometry instruments that are available. These reagent products are considered to have a purity level that is above 90%.
The eBioscience reagents for flow cytome
Merck KGaA located in Darmstadt, Germany is a provider of technology products for life science, healthcare, and performance materials industries. EMD Millipore represents the company’s life science division. The company is a provider of several products that include reagents, industrial microbiology, small molecule pharmaceuticals, and life science research.
EMD Millipore’s portfolio of inorganic reagents comply toReag.PhEur and ACS specifications and are optimized to be used in wet chemical analysis. The reagents support a wide range of biochemical analysis needs that refer to standards, quantities, application. The inorganic reagents that are developed by EMD Millipore are designed specifically for classical inorganic analysis and instrumental inorganic analysis. The company also provides safety and general products to support their reagent deliveries. The group for classical inorganic analysis offer a variety of
Sigma-Aldrich develops life science products in the form of biochemical and reagents. The antibody products include flow cytometry antibodies. Antigens, lgY antibodies, antibodies for proximity ligation assays, and antibody kits. The primary antibodies for flow cytometry that Sigma-Aldrich develops are monoclonal. The antibodies are available in two forms: conjugated or purified. The company also produces other reagents such as blocking agents, controls, and purified immunoglobulins.
Sigma-Aldrich also provides antibodies in whole molecule form and as fragments known as F(ab)2. In addition to whole antiserum and F(ab)2antibodies, Sigma-Alrich produces numerous types other common types of antibodies such as fractionated antiserum, IgG fraction of antiserum, affinity isolate antibody (AIA), ascites fluid, tissue culture supernatant, and purified immunoglobulin.
The primary antibodies that are developed by Sigma-Aldr
Jackson ImmunoResearch focuses their antibody development on secondary antibodies. Their range of products includes antibodies for flow cytometry, primary antibodies that are used for signal enhancement, and whole lgG affinity-purified antibodies.
The whole lgG affinity-purified antibodies are supported for numerous target species that include mouse, rabbit, sheep, rat, human, cat, chicken, and dog. This group of antibodies includes several types of applications. The anti-mouse lgG subclass antibodies group is used to differentiate multiple subclasses of the mouse lgG in numerous experiments. They can also be used to determine the lgG mouse subclass. The R-PE, APC, and PerCP conjugates are proteins for flow cytometry. Other categories of whole lgG affinity-purified antibodies include anti-light chain-specific antibodies, Alex Fluor® 680 and Alexa Fluor® 790 antibodies, Cyanine dyes, colloidal gold reagen
Abcam develops primary antibodies that consists of polyclonals, monoclonals, flow cytometry antibodies, and conjugated antibodies. The polyclonals group of primary antibodies include those that detect antigens of immune systems of rabbits, mouse, goats, and chickens. Abcam’s monoclonals are antibodies that are associated with a specific epitope that is within an antigen. These antibodies include rabbit monoclonals, mouse monoclonals, rat monoclonals, and hamster monoclonals. Abcam’s flow cytometry antibodies are developed to work with a variety of cell targets. The company provides a flow panel selector that allows researchers to search their catalog of flow cytometry antibodies from their primary antibodies group. The conjugated antibodies are developed using the company’s own RabMab® technology. The process of developing these antibodies involves separating free dye to maximize the signal-to-noise ratio.
Tonbo Biosciences has been in existence since 2011 when it was started by various researchers and immunologists. The company’s products include antibodies and reagents, PCR reagents, recombinant and proteins. Tonbo Biosciences also provide products with research focus and custom and bulk reagents.
The group of antibodies and reagents includes reagents for flow cytometry, buffers and solutions, In Vivo Ready ™ reagents, Ready-Set-Flow™ and backbone panels. The Tonbo flow cytometry reagents have reactivity with rat, mouse, and human cell targets. They are available in a variety of formats that include FITC, Biotin, PE, PE-Cy5, APC, and PerCp. The Tonbo Biosciencs process for manufacturing reagents for flow cytometry involves enhancing the ratio of protein-to-fluorophore, performing quality-control testing, and adhering to stringent final release criteria. The applications of the flow cytometry reagents include IHCP, IH
SouthernBiotech, formerly known as Southern Biotechnology Associates, Inc., started its operations in the early 1980s with scientists that worked in the Dr. Max Cooper Cellular Immunology Laboratory at the University of Alabamain Birmingham. SouthernBiotech began its company by developing and acquired monoclonal antibodies from other institutions and universities. Today, their list of products include monoclonal and polyclonal antibodies, collagens, and immunoglobulins, and fluorochrome conjugates. SouthernBiotech offers products to countries in Europe, Asia, Africa, South America, and the Middle West through a network of distributors that include Fisher Scientific that is located in the United States.
SouthernBiotech provides numerous categories of products. The polyclonal secondary antibodies and reagents category includes antibodies and reagents that are suitable for a variety of species such as human, sheep
Beckman Coulter, Inc. is a company that provides instruments, software, chemistry, and research supplies to laboratory researchers. The company was founded in 1935 by Dr. Arnold O. Beckman who developed the original PH meter. The company later developed DU spectrophotometer and heliport potentiometer. By the 1990s, the company had expanded its product offerings to include laboratory instruments, immunoassay, and hematology, hemostasis, flow cytometry, and diagnostics.
Beckman Coulter provides products for biomedical research analysis. The categories of these products include support reagents, human antibodies, and non-human antigens.The support reagents include secondary antibodies, isotypic controls, detection kits, ancillary reagents, high affinity molecules, fluorospheres and controls, and instrument support reagents. Beckman Coulter offers more than 700 antibodies. These antibodies are available for several
Rockland Antibodies develop primary, secondary, green fluorescent protein (GFP), epitope, and signaling antibodies. Primary antibodies are significant for research projects that involve diagnostic testing. In addition to these specific antibodies, Rockland Antibodies also develops custom antibodies.
The company’s primary antibodies are generated for detecting antigens of a specific type. These types include peptides, carbohydrates, and proteins. The primary antibodies are conjugated and unconjugated, which are determined by their assay format. The primary antibodies developed by Rockland Antibodies have several applications. Researchers can use the conjugated primary antibodies to alleviate the requirement to use a secondary antibody. The antibodies are also applicable for recognizing antigens for several assay formats such as analytical cytology, neutralization, crystallography, and immunoprecipitation (IP)
Santa Cruz Biotech
Santa Cruz Biotechnology is a provider of numerous groups of reagents that are suitable for numerous types of research projects. The company’s suite of research products include primary antibodies, secondary antibodies, proteins, tissue extracts and cell lysates, and veterinary research antibodies.
One group of primary antibodies that Santa Cruz Biotech produces is directed for a variety of both non-mammalian and mammalian protein targets. These antibodies are available in numerous functional groups that include cell cycle proteins, homeodomain proteins, steroid receptors, neurobiology, channel proteins, fusion protein tags, cell adhesion proteins, kinases and phosphatases, and signaling intermediates.
Santa Cruz Biotech offers a group of phospo-specific antibodies. These antibodies are explicitly or threonine, serine, phosphorylated amino acids tyrosine. They are also specific to a variety of monoclonal a
CST Cell Signaling Technology
In the late 1990s, a group of research scientists founded Cell Signaling Technology (CST) to develop antibodies for applied systems biology research and to provide support to researchers. CST develops primary antibodies, secondary antibodies, antibody conjugates, ELISA, and antibody arrays. The company also tests these antibodies in-house. The tests are to confirm reproducibility, specificity, and sensitivity are at acceptable levels. These antibodies are suitable for a range of applications that include western blotting and flow cytometry.
The primary antibodies include Akt, ALK, Caspase, MAPK, mTOR, and NF-kB. Akt antibodies are more commonly referred to as PKB, and is a key factor in various cellular processes. These processes include insulin metabolism and cancer progression. The Akt antibody exists in three isoforms. The class of Caspase products are essential for regulating apoptosis. The Ini
Dako was founded in 1966 by Niels HarboeDako, a Danish medical doctor. In 2012, the company was acquired by Agilent Technologies. Dako, now an Agilent Technologies company, is a provider of a variety of products that support biomedical analysis projects. Their offerings include reagents, antibodies, instruments, and software. In addition to instruments Dako develops products that belong to the following areas: flow cytometry, molecular pathology, pharmacoDiagnostic®, and immunohistochemistry (IHC).
The Dako flow cytometry products include primary single-color reagents, dual color reagents, isotype and control reagents, MultiMix™ Triple Color Reagents, secondary antibody conjugates, and streptavidin conjugate, and kits. The primary single-color reagents have a single flurochrome that they are conjugated with for researchers to use in flow cytometry research projects. Gel filtration causes the unreacted fluorochrom