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How To Use A Threshold To Reduce Background Noise In Flow Cytometry

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Getting a clear signal with reduced noise is an essential component to good data. Adding a threshold when acquiring flow cytometry data is one way to do that. It reduces the number of events by setting a bar that a signal pulse must clear before it is counted as an event. Depending on the importance of the data, the downstream applications for the data (or sorted cells) will dictate how critical the threshold is. In combination with proper sample preparation, appropriate thresholding will reduce debris and ensure best outcome.

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How To Set And Monitor Optimal Voltages For A Flow Cytometry Experiment

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The best way to take out the fear and agony of setting voltages is to use some optimization methods. The peak 2 method is a useful and robust method of identifying optimal PMT voltage ranges. Refining that to the voltage walk with the actual cells and fluorochromes of interest will further improve sensitivity, which is especially critical for rare cell populations or emergent antigens. This article describes how to set up, monitor, and maintain optimal voltage settings for your flow cytometry experiment.

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How To Prepare For A Flow Cytometry Experiment

Preparation is vital before starting a flow cytometry experiment
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Flow cytometry is a very powerful tool and can answer many questions if the experiments are properly designed. There is a learning curve that takes a bit of time, patience, and practice, but soon you may be finding excuses to perform flow cytometry experiments and we will be here to help you with best practices. Using this checklist will help you to design and perform consistent experiments every time.

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How To Troubleshoot The Flow Cytometer Fluidics System

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Most of the interactions that a user has with a flow cytometer is with the fluidics system, and many of the issues that users will face in troubleshooting problems on the instrument will also be here. Understanding how the fluidics system works on your flow cytometer will help you prevent many common issues, prepare your samples correctly, and protect your data. Here are four important things to consider about the fluidics system in a flow cytometer.

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How To Get A Flow Cytometry Job In 5 Steps

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Flow cytometry is a powerful technique impacting both clinical and research. When looking for a career, flow cytometry can take you many places. An experienced flow cytometrist can find a job in a biotechnology company, academia, a clinical setting, and more. To be successful in the field, it’s important to seek out new educational opportunities and network with your peers. Here are 5 tips that can help you turn flow cytometry into a successful career.

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