Meet Your Instructor
Ryan Duggan is primarily a Flow Cytometry professional. He serves as the Technical Director of the Flow Cytometry Core Facility at the University of Chicago (UCFlow). He writes under multiple aliases including RynDggn (personal) and UCFlow (professional).
He is probably best known for his critical evaluation of flow cytometry technology and support services. Most of this information can be found on the UCFlow Blog. He also serves as a technology consultant for numerous equipment and reagent manufacturers, Biopharma, tranlational research groups, and other educational institutions.
Derek runs the Flow Cytometry Laboratory at the London Research Institute, which is part of Cancer Research UK. He has been in the cytometry field since 1980 and in flow cytometry since 1985. Since then, Derek has run a huge variety of analysers and cell sorters. He has particular expertise in cell cycle and cell death analysis, but also in complex phenotyping, rare event analysis, and functional assays, such as calcium flux.
Derek devotes a large part of his time to teaching. All new users to his facility undergo a training program. In the wider community, Derek teaches on, and organizes, several established courses in the UK. Derek is the Chair of flowcytometryUK, the UK’s National Society, and sits on the Council of the Royal Microscopical Society (RMS) and the International Society for the Advancement of Cytometry (ISAC).
Alfonso Blanco, Ph.D.
Dr. Alfonso Blanco is the Scientific Director of the Conway Core Facilities, which provides technical, educational and scientific support to a very large variety of groups, institutions and private companies across Ireland. Alfonso obtained his B.Sc. in Biology in 1995 from the University of Santiago de Compostela, Spain, and his M.Sc. and Ph.D. Biology from the Universidade de Vigo, Spain, in 1997 and 2003 respectively. He joined the UCD – Core Technology Unit of the UCD – Conway Institute of Biomolecular and Biomedical Research in May 2004 in order to develop the Flow Cytometry Core Technology (FCCT) and research projects specific to this technology. Alfonso is involved in many educational and training programs, such as the International Summer Schools organized by the European Society of Clinical Cytometry Analysis (ESCCA). During the 7th European Clinical Cytometry Course in September 2011, he has been involved in the organization of the first on-line cell sorting training course using remote sites and he is one of the leaders of an international educational initiative for eLearning. In 2011, Dr. Blanco has been incorporated as one of the board members of the ESCCA Website & Communication Committee. In 2012 Alfonso was elected as one of the councilor of the International Society for Advancement of Cytometry (ISAC) and is working as one of the members of the ISAC Educational Task Force and Programming Committee of CYTO. Alfonso is finalist in two categories of the Irish Laboratory Awards 2013: “Educational Laboratory of the Year” and “Laboratory Staff Member of the Year”.
Rui Gardner, Ph.D.
With a background in Biochemistry, Rui started his career in science as a mathematical biologist working in the field of Reactive Oxygen Species metabolism. His PhD work on ROS was carried out at the University of Michigan, University of Southern California, and at the Gulbenkian Institute of Science in Portugal. This was followed by postdoctoral work in immune diversity estimation techniques, still at the Gulbenkian Institute, where in 2007 he became Head of the Flow Cytometry Core Facility. Serving more than 40 research groups in many diverse fields including Immunology, Inflammation, Stem-Cell Biology, Microbiology, Virology, Plant Biology, among others, has given Rui a comprehensive experience in many Flow Cytometry techniques. Passionate about Science and Technology, Rui’s background in Mathematics, Physics, and Biology allows bridging the gap between the operational and technical details in flow cytometry and the science within flow applications. He has lectured many courses on Flow Cytometry and Cell Sorting, and continuously involved in organizing various workshops and meetings on Flow Cytometry and Core Management in Europe. Rui has been a member of the International Society for the Advancement of Cytometry (ISAC) since 2007, and was elected Councilor in 2012, being actively involved in ISAC’s Shared Resource Labs Task Force. Rui is also a member and Councilor of the Sociedad Iberica de Citometria (SIC) and both member and co-administrator of the European Cytometry Network (ECN).
Matt is the Technical Director of the URMC Flow Cytometry SRL. The lab contains eight traditional analytical flow cytometers, two cell sorters, an Image Stream X imaging cytometer and a CyTOF mass cytometer and serves the upstate NY region from Buffalo to Ithaca. Matt began his career in flow cytometry in 2004 in the Rochester Human Immunology Core lab managing an 8-color LSRII and has been dedicated to the field ever since. He has experience with many traditional and non-traditional cytometers and a wide variety of applications including large-scale immunophenotyping and panel design. In addition to the Rochester cytometry training program which he’s been running for ~8 years he’s been teaching and consulting nationally for a number of years. Matt is also a member of the ISAC shared resource laboratory task force, the ABRF flow cytometry research group, and the GLIIFCA steering committee.
James McCracken, Ph.D.
James McCracken is the Technical Director of the University of Louisville Diabetes and Obesity Center Flow Cytometry Core and an expert in apoptosis studies. Over his 15 years of increasing exposure to flow cytometric methods, he has gone from using the technology as another tool in the box for graduate and postdoctoral work to making it his professional passion. At the DOC since 2010, he aids in experimental design and analysis, grant applications, acquisition of new equipment, and training of all users. James completed his undergraduate studies in Biology at Emory and Henry College in Virginia and completed a PhD. in Microbiology and Immunology from Tulane University. After a postdoctoral appointment at the University of Chicago. When he is not in the lab, he is fond of cooking, travel, and making friends with cats.
Kylie Price obtained a BSc majoring in Biochemistry from the University of Otago in 2000 and Master of Science in Cell and Molecular Bioscience from Victoria University in Wellington, New Zealand, in 2003. Having completed her MSc, she wanted to get work experience at New Zealand’s leading biomedical research centre, the Malaghan Institute of Medical Research. Initially she joined the Institute’s Multiple Sclerosis team before the opportunity came up to take over the Flow Cytometry facility in 2005. In 2012 she undertook a 6-month “technology transfer sabbatical” in Spain, spending time in some of the most advanced flow cytometry facilities in Spain and Europe; the Spanish National Cancer Research Centre (CNIO) in Madrid and Barcelona’s Biomedical Research Park (PRBB). She continues to manage the Malaghan Institute’s cell technology suite, which encompass flow cytometry, microscopy, and immunohistochemistry. She has been credited with pioneering the use of multicolour flow cytometry within New Zealand and enjoys using flow cytometry for as wide a range of research applications as possible. She has analysed and sorted: side-population cells from primary human brain tissue, bacteria from Antarctic sea-ice, bovine sperm and algal spores, to name a few. She is also a member of the Proliferation Content Author Group of the CYTO University Flow Cytometry Content Task Force of the International Society for Advancement of Cytometry (ISAC) and is the current President Elect of the Australasian Cytometry Society (ACS).
Adrian Smith, Ph.D.
Adrian has been the manager of the Cytometry and Imaging Facility at the Centenary Institute since 2004. He has overseen the growth of the facility from three flow cytometers in 2004 to now include over twenty instruments including a range of advanced optical microscopes and image cytometers, as well as traditional flow cytometers and cell sorters - including the world's first 10 laser analyser and first 10 laser cell sorter. Adrian is also the Technical Director of the Advanced Cytometry Facility - a joint of initiative of Centenary and the University of Sydney. Adrian has been the president of the Australasian Cytometry Society (ACS) (formerly the Australasian Flow Cytometry Group (AFCG)) since December 2010 and in May 2014 was elected to the council of the International Society for the Advancement of Cytometry. Adrian teaches imaging and cytometry on a regular basis to both researchers and technical staff and he has presented workshop and tutorials for many groups including CSIRO, AFCG/ACS, QIMR, ASI, ISCT and ISAC (CYTO conference). He has trained several core facility staff who are now managing their own facilties. Adrian started in flow cytometry nearly 20 years ago and his PhD in Immunology (peripheral CD4 T Cell responses) included more hours in front of a flow cytometer than he cares to (or can) remember. When he is not looking down a microscope or sitting in front of cytometer he can be found with a camera in hand chasing the perfect shot.
Charlotte Christie Petersen, Ph.D.
Charlotte has been the manager of the FACS Core Facility at Aarhus University in Denmark since 2010. The core facility is equipped with two FACSAriaIII cell sorters, an LSRFortessa and a NovoCyte analyzer, and in addition, an ImageStream X Mark II. Charlotte has a master’s degree in chemistry and molecular biology followed by a Ph.D. in immunology. She has worked with flow cytometry since 2001 and cell sorting since 2010. Before being a core manager, she mainly worked with the adaptive immune system. Adaptive immunotherapy with tumor-specific T cells and immune responses to viral infections have been her main focus. The last 7 years Charlotte has been a co-organizer and teacher on the Ph.D. course in flow cytometry at the faculty of Health, Aarhus University, Denmark. Charlotte is a member of ISAC and vice-president in the Danish Society for Flow Cytometry.
Joanna’s interest in flow cytometry began during her work in the mid nineteen nineties at the Malaghan Institute of Medical Research in Wellington while establishing a mouse model of influenza infection to characterise T and B cell immune responses with Prof Graham Le Gros. Working as as Research Officier with Prof Franca Ronchese following this, lead to further projects using flow cytometry, followed by the opportunity to set up the Malaghan Institute Flow Cytometry Suite with the purchase of the first digital cell sorter for immunology in NZ. Following several years as Staff Scientist responsible for Flow Cytometry at the Malaghan, Joanna was appointed Head of the Flow Cytometry Platform at the Federal Institute of Technology (EPFL), in Lausanne, Switzerland.
Jean-François is the Chief Scientific Officer of TransCure bioServices SAS a fee for service company providing access to a humanized mice model (human immune system) for pre-clinical studies at Archamps, near Geneva. He is also responsible of the cell biology and cytometry unit. Jean-François obtained his PhD in the Grenoble University, France and worked during 14 years for the French Army Health Service as a specialist of Stem Cells RadioBiology and as the responsible of the cytometry platform. He developed a deep knowledge in several fields like Stem Cells biology, Immunology, cell death, proliferation, cell cycle. He is involved in several cytometry courses in France, Europe and north Africa like the frenchInter Univerisity Diploma of Clinical and Research Cytometry and the ESCCA summer school. As an active member of the French Society of Cytometry (Association Française de Cytométrie – AFC), Jean-François was involved in the organization and annual congress, thematic workshop, was elected in the AFC’s board as secretary and vice-president from 2006 to 2011. He is also a member of ESCCA and member of the ESCCA Membership Affair Committee ESCCA finances. Jean-François co-edited two books in French about flow cytometry.