Differential pressure based flow cytometers currently dominate the market. These systems have two pressure regulators. The first is at a constant pressure that sets how fast the fluids runs at. The second is regulated by the investigator (like as shown on this LSR-II control panel).
As the sample pressure goes from low, to medium, to high, the pressure on the sample increases. This results in the volume of the sample increasing (from ~15 ml/min to ~60 ml/min).
The difference between the sample pressure and the sheath pressure is the differential pressure. This controls the width of the core stream and the total number of cells passing the laser intercept.
My other passions include grilling, wine tasting, and real food. To be honest, my biggest passion is flow cytometry, which is something that Carol and I share. My personal mission is to make flow cytometry education accessible, relevant, and fun. I’ve had a long history in the field starting all the way back in graduate school.
Latest posts by Tim Bushnell (see all)
- 3 Compensation Mistakes That Will Ruin Your Flow Cytometry Experiments - January 2, 2020
- We Tested 5 Major Flow Cytometry SPADE Programs for Speed – Here Are The Results - December 5, 2019
- Mass Cytometry Revolves Around These 5 Things - November 21, 2019