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Flow Cytometry Bootcamp at The University of California Santa Cruz

September 8, 2014 @ 9:00 AM - September 9, 2014 @ 5:00 PM

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Educational Objectives

This two day course will cover the fundamentals of flow cytometry to provide critical concepts for the novice and intermediate user. This course focuses on the core concepts of flow cytometry ‐ the ‘basics’ that every scientist using flow cytometry should know. At the end of this course, students will have a better understanding of the complexities of flow cytometry experimental design, optimization, and analysis.

Target Audience

Users with basic flow cytometry understanding looking to learn the primary concepts of flow cytometry and a desire to move to higher end assays (from 4 color experiments to 8++ color). Learning the fundamentals will ensure that your flow cytometry experiments will yield results ‐ which saves you time and money in the long run.

Course Outline/Schedule – Day 1

09:00­-09:15 ­ Course Welcome and Introductions

09:15­-10:00 ­ Warm it up! Fluorescence, Fluorochromes and Reagents

10:00­-10:30 ­ Activity 1: Fluorescence and Spectral Viewers

10:30-­10:45 ­ Coffee Break

10:45­-11:30 ­ Work it out. Flow cytometry hardware

11:30­-12:15 ­ Show it off! Data analysis for flow cytometry

12:15­-12:30 ­ Quiz 1 and questions from the morning session

12:30­-13:30 ­ Lunch

13:30-­14:30 ­ Keep cool. Controls in cytometry

14:30­-15:15 ­ Ready for competition? Compensation at its core

15:15­-15:30 ­ Coffee Break

15:30­-16:00 ­ Activity 2: Compensation practice

16:00-­16:45 ­ Time for flair! Theory of multicolor panel design

16:45­-17:00 ­ Quiz 2 and questions from the afternoon session

Course Outline/Schedule – Day 2

09:00­-09:45 ­ Activity 3: Panel Design

09:45­-10:30 ­ Take it to the limit! Critical parameters in experimental optimization

10:30­-10:45 ­ Coffee Break

10:45-­11:45 ­ How mean are you? Statistics in flow cytometry

11:45­-12:30 ­ Activity 4: Data analysis

12:30­-13:30 ­ Lunch

13:30­-14:30 ­ Ready for the Fashion Runway? Data modeling from simple to complex

14:30­-15:30 ­ Survey says! Survey of flow cytometry applications

15:30­-15:45 ­ Coffee Break

15:45­16:45 ­ 99 problems, but flow ain’t one! Identifying solving problems

16:45­-17:00 ­ Final questions and course wrap up

Santa Cruz Flier

 

Tim Bushnell

Tim Bushnell

I enjoy answering paradigm-shifting questions and trouble-shooting puzzling glitches. I also like finding new ways to enhance old procedures. I’m passionate about my professional relationships and strive to fill them with positive energy.

My other passions include grilling, wine tasting, and real food. To be honest, my biggest passion is flow cytometry, which is something that Carol and I share. My personal mission is to make flow cytometry education accessible, relevant, and fun. I’ve had a long history in the field starting all the way back in graduate school.
Tim Bushnell

Details

Start:
September 8, 2014 9:00 AM
End:
September 9, 2014 5:00 PM
Event Category:

Venue

University of California Santa Cruz
1156 High Street, Santa Cruz, CA 95064 United States
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