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How Flow Cytometry Converts Photons To Digital Data

Flow Cytometry conversion of photons to electrons
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From photon, through conversion from light, into an electron, into an electronic signal pulse. The journey of a photon, through to its data representation via flow cytometry, involves the detection system and the electronics, and basically following a bouncing photon to its ultimate digitization. This article provides 3 key takeaways in the process from photons through to end data for analysis.

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How To Use Flow Cytometry To Analyze Rare Cells Within Heterogeneous Samples

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Poorly designed panels, failure to plan for the end-results, and not taking into account instrument characteristics, can all result in a failed sort, lost opportunity, and delay in the necessary data. Take the time at the beginning, before starting a rare event sorting, to understand the different issues that will potentially impact your outcome, and develop a plan to address each of them. Here are 4 considerations to keep in mind.

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How To Troubleshoot The Flow Cytometer Fluidics System

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Most of the interactions that a user has with a flow cytometer is with the fluidics system, and many of the issues that users will face in troubleshooting problems on the instrument will also be here. Understanding how the fluidics system works on your flow cytometer will help you prevent many common issues, prepare your samples correctly, and protect your data. Here are four important things to consider about the fluidics system in a flow cytometer.

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Why Flow Cytometry Fluorescence Compensation Is Critical For Quality Measurements

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Fluorescence compensation is one of the more difficult, understandably confusing, and misunderstood aspects of flow cytometry. Understanding what compensation is, why it is necessary, and what to expect when using it, are critical for generating useful and high-quality data from flow cytometry experiments. The definition and mechanics of flow cytometry compensation and the critical concept that compensation’s most basic principle relies on are discussed here.

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What Is A Flow Cytometry Laser And How Flow Cytomtery Optics Function

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Understanding the optical system of a flow cytometer may seem unnecessary for performing a typical experiment, but the more you know about your instrument, the better you will be at understanding your data, as well as troubleshooting potential issues. This article breaks down 4 elements of flow cytometer optics to provide a broad understanding on its impact on fluorescence.

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