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5 Best Practices For Accurate Flow Cytometry Results

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Here, we cover 5 lessons from the trenches of flow cytometry looking at important aspects of how best practices have changed over time, which practices need to be adopted, and which are outdated. Put those old, coffee-stained protocols away and take advantage of the best practices for digital instruments to write new and improved ones (coffee stains optional). Your data will thank you.

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5 Essential Calculations For Accurate Flow Cytometry Results

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Flow cytometry is a numbers game. There are percentages of a population, fluorescence intensity measurements, sample averages, data normalization, and more. Many of these common calculations are useful, but surrounded by misconceptions. This primer will help you decide which calculation to use, when to use it, and how to interpret the results.

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How Flow Cytometry Converts Photons To Digital Data

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From photon, through conversion from light, into an electron, into an electronic signal pulse. The journey of a photon, through to its data representation via flow cytometry, involves the detection system and the electronics, and basically following a bouncing photon to its ultimate digitization. This article provides 3 key takeaways in the process from photons through to end data for analysis.

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How To Use Flow Cytometry To Analyze Rare Cells Within Heterogeneous Samples

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Poorly designed panels, failure to plan for the end-results, and not taking into account instrument characteristics, can all result in a failed sort, lost opportunity, and delay in the necessary data. Take the time at the beginning, before starting a rare event sorting, to understand the different issues that will potentially impact your outcome, and develop a plan to address each of them. Here are 4 considerations to keep in mind.

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How To Troubleshoot The Flow Cytometer Fluidics System

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Most of the interactions that a user has with a flow cytometer is with the fluidics system, and many of the issues that users will face in troubleshooting problems on the instrument will also be here. Understanding how the fluidics system works on your flow cytometer will help you prevent many common issues, prepare your samples correctly, and protect your data. Here are four important things to consider about the fluidics system in a flow cytometer.

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