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Learn everything you need to know about setting up, running, and analyzing a flow cytometry cell sorting experiment, including: The 3 most important questions you [...]
Learn everything you need to know about setting up, running, and analyzing a flow cytometry cell sorting experiment, including:
- The 3 most important questions you need to answer BEFORE starting your cell sorting experiment.
- How to calculate sort recovery, yield, and purity.
- How to correct cell sorting problems including improper laser alignment, clogged nozzels, and incorrect population gating.
- How to solve problems related to differential pressure and trigger settings.
- Which cell sorting numbers, units, and statistics are important and how to use them to get your cell sorting data published.
- How to understand Poisson distributions and how this understanding can correct your cell sorting experiment
- Complete information on which tubes to use, which sheath fluid to use, which nozzle size is best for your experiment, and whether or not you should use viability dyes for your experiment.
- How to prepare the perfect catch buffer for the cells you will be sorting in your experiment
- 5 insider tips that will improve your very next cell sorting experiment including the one inhibitor that will prevent your cells from clumping even after neutralizing them with FBS.
- Tips for getting your flow cytometry cell sorting data published after you collect it.
*This webinar will be recorded and sent to you if you join and can’t make it to the live event.
The history of the electrostatic cell sorting started in 1965 when Mack Fulwyler combined the identification cells using the Coulter Principle (Wallace H. Coulter) with the electrostatic deflection principles developed for the inkjet printer (Richard Sweet). This was extended when a PMT based fluorescent detection system was added and the modern cell sorter was born.
The range and power of current cell sorters include multi-laser, multi-detector systems with the ability to sort up to 6 ways or into microwell plates. All these instruments are governed by the same physics, regardless of what the vendor may say. Experimental needs drive the choice of instrument.
Electrostatic Cell Sorting may seem like an art, but understanding several important principles in sample preparation and cell sorter operation can easily move frustration to fruition.
(Thursday) 1:00 PM
Isaiah Hankel, Ph.D. and Tim Bushnell, Ph.D.
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This is a test event
29 (Saturday) 1:00 AM - 31 (Monday) 1:00 AM cst
1085 Budapest, Hungary
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