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5 Special Considerations for Live Cell Imaging

Live cell imaging is advantageous for research were you may be worried about artifacts of fixation or when you want to measure a phenomenon over time. Live cell imaging is more difficult to achieve than fixed samples because we need to keep the cells live AND happy along with obtaining the images we need. We can reduce artifacts by keeping the cells in a favorable environment and minimizing external stressors. Here are 5 points to keep in mind when setting up your live cell imaging experiment.

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5 Steps To Improve Your Flow Cytometry Data Analysis

To get the best flow cytometry data you need to be thinking about all the steps in your experiment to ensure that you have high-quality data to analyze. To improve the quality of your analysis make sure you’re adding keywords at the beginning of your experimental setup, develop a quality control program, trust but verify any software wizards, use proper controls, and make sure you extract the correct data.

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The Right Way To Read A Flow Cytometry Scientific Paper

Reproducibility is a state of mind. It’s not one simple thing that you do that will make all your data more reproducible, it a shift in the way one thinks about and perform experiments. With the emphasis on rigor and reproducibility in science, it’s very important that researchers start putting into place everything they can do to help improve the quality and reproducibility of there data. Learn 3 action steps that can be taken to enhance experimental reproducibility.

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Avoid Data Loss By Following These Steps To Set Your Flow Cytometry Gates Correctly

The way you set your gates in flow cytometry is a key part of your experiment. You need to know that you can find the populations that you’re interested in so you can extract the appropriate data. This is how you can do your secondary or statistical analysis confidently. Learn what gates you should be using and how to define them properly.

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3 Ways To Measure Cell Death With Flow Cytometry

Cell death is a natural part of the lifecycle of a cell. In cases of development, it is critical for the shaping of fingers during human development. The processes of ordered cell death, or Apoptosis, are so important that in 2002, Sidney Brenner, Robert Horvitz, and John Sulston received the Nobel Prize in Medicine for their work on understanding this process. There are many different ways to measure cell death and flow cytometry is an ideal tool for this technique. Whether you are just assessing the viability of your cells or you are interested in the exact stage of cell death your sample is in, there are a variety of ways that you can measure cell death. Learn 3 ways you can use flow cytometry to measure cell death.

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