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    Flow Cytometry Bootcamp at University of Wisconsin

    May 5 @ 9:00 am - May 6 @ 5:00 pm

    Educational Objectives

    This two day course will cover the fundamentals of flow cytometry to provide critical concepts for the novice and intermediate user. This course focuses on the core concepts of flow cytometry – “the basics” that every scientist using flow cytometry should know. At the end of this course, students will have a better understanding of the complexities of flow cytometry experimental design, optimization, and analysis.

    Target Audience

    Users with basic flow cytometry experience and a desire to move to higher end assays (from 4 color experiments to 8++ color)

    Course Outline/Schedule – Day 1

    09:00-09:15 Introductions and course overview

    09:15-10:15 Warm it up – Antibodies and Fluorochromes

    10:15-10:30 Break

    10:30-11:15 How it all works. Conquering the flow cytometer.

    11:15-11:45 Ready for Competition? Compensation at its core.

    11:45-12:00 Questions from morning session

    12:00-13:00 Lunch

    13:00-14:00 Taking Control – (in cytometry) – Types of Controls in Flow Cytometry

    14:00-15:00 Show your true colors! Theory of multicolor panel design

    15:00-15:15 Coffee Break

    15:15-16:00 Take it to the limit! Critical parameters in experimental optimization

    16:00-16:45 Practice makes perfect! Applying theory to practical panel design.

    16:45-17:00 Course wrap-up and questions from Day 1

    Course Outline/Schedule – Day 2

    09:00-09:30 Introductions and course overview/ review from day 1

    09:30-10:30 Data Power! – Flow data structure, display and gating

    10:30-10:45 Coffee Break

    10:45-11:45 Just how mean are you? Statistics in flow

    11:45-12:00 Questions from morning session

    12:00-13:00 Lunch

    13:00-14:00 Time to Model. Data modeling from simple to complex

    14:00-15:00 The Awards go to… Presenting your data

    15:00-15:15 Break

    15:15-16:00 Survey Says! – a survey of flow cytometry applications

    16:00-16:45 99 problems, but flow ain’t one! Identifying and solving problems

    16:45-17:00 Course wrap-up

    Universtiy Wisconsen Flier

    Details

    Start:
    May 5, 2014 9:00 am
    End:
    May 6, 2014 5:00 pm
    Event Category:
    Cytometry Consulting and Education - Affordable. Effective. Leading Edge.