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Flow Cytometry Bootcamp at University of Wisconsin

May 5, 2014 @ 9:00 AM - May 6, 2014 @ 5:00 PM

Educational Objectives

This two day course will cover the fundamentals of flow cytometry to provide critical concepts for the novice and intermediate user. This course focuses on the core concepts of flow cytometry – “the basics” that every scientist using flow cytometry should know. At the end of this course, students will have a better understanding of the complexities of flow cytometry experimental design, optimization, and analysis.

Target Audience

Users with basic flow cytometry experience and a desire to move to higher end assays (from 4 color experiments to 8++ color)

Course Outline/Schedule – Day 1

09:00-09:15 Introductions and course overview

09:15-10:15 Warm it up – Antibodies and Fluorochromes

10:15-10:30 Break

10:30-11:15 How it all works. Conquering the flow cytometer.

11:15-11:45 Ready for Competition? Compensation at its core.

11:45-12:00 Questions from morning session

12:00-13:00 Lunch

13:00-14:00 Taking Control – (in cytometry) – Types of Controls in Flow Cytometry

14:00-15:00 Show your true colors! Theory of multicolor panel design

15:00-15:15 Coffee Break

15:15-16:00 Take it to the limit! Critical parameters in experimental optimization

16:00-16:45 Practice makes perfect! Applying theory to practical panel design.

16:45-17:00 Course wrap-up and questions from Day 1

Course Outline/Schedule – Day 2

09:00-09:30 Introductions and course overview/ review from day 1

09:30-10:30 Data Power! – Flow data structure, display and gating

10:30-10:45 Coffee Break

10:45-11:45 Just how mean are you? Statistics in flow

11:45-12:00 Questions from morning session

12:00-13:00 Lunch

13:00-14:00 Time to Model. Data modeling from simple to complex

14:00-15:00 The Awards go to… Presenting your data

15:00-15:15 Break

15:15-16:00 Survey Says! – a survey of flow cytometry applications

16:00-16:45 99 problems, but flow ain’t one! Identifying and solving problems

16:45-17:00 Course wrap-up

Universtiy Wisconsen Flier

Tim Bushnell

Tim Bushnell

I enjoy answering paradigm-shifting questions and trouble-shooting puzzling glitches. I also like finding new ways to enhance old procedures. I’m passionate about my professional relationships and strive to fill them with positive energy.

My other passions include grilling, wine tasting, and real food. To be honest, my biggest passion is flow cytometry, which is something that Carol and I share. My personal mission is to make flow cytometry education accessible, relevant, and fun. I’ve had a long history in the field starting all the way back in graduate school.
Tim Bushnell


May 5, 2014 9:00 AM
May 6, 2014 5:00 PM
Event Category:
Flow Cytometry Education And Consulting - Affordable. Effective. Leading Edge.