5 Flow Cytometry Errors Reviewers Despise
We all know that flow cytometry makes individual measurements on large populations of cells, it allows for statistical analysis of the data, lending strength to a researcher’s conclusions.
Likewise, the isolation of very complex populations by flow cytometry cell sorting can help lead to a richer understanding of the intricate biology at the genomic, proteomic and functional level.
As a reviewer of papers and grants, I am always especially interested in the details of HOW the experiments were performed because that is the critical foundation for what the data is able to tell us–and what it can NOT tell us.
Mistakes That Mark You As Untrained
It may sound harsh but there are some errors that reviewers hate seeing. The good news is that by learning what reviewers dislike (or even despise), you can learn how to run better and more accurate experiments. Here are 5 errors... Read More
The Most Common Mistake Researchers Make When Designing Flow Cytometry Antibody Panels
Pairing highly expressed antigens (like CD3) with dimmer fluorochromes, and the antigens of interest with the brightest fluorochromes, is a key part of panel design with few tools to help.
With early generation instruments, this was relatively easy to determine, since fluorochrome choice was limited. With the advent of instruments capable of measuring more than 4 fluorochromes, there is a need to characterize the relative brightness of different fluorochromes under actual experimental conditions, rather than as free fluors.
Bigos et al (2004) first reported this in an abstract and it was later simplified in Maecker et al (2004). This equation... Read More
Top Flow Cytometry Instruments, Reagents & Software To Look Forward To In 2015 (1st Annual List)
Here at ExCyte–we are excited for all of the new flow cytometry instruments, reagents, and software you will have access to in 2015.
There are so many new and innovative products that we have been testing and looking into for you. These are just a few highlights of the great things coming your way in 2015.
The field of flow cytometry is seeing various trends in instrumentation–on one end are smaller, more compact and more powerful instruments for the high end needs of researchers, and on the other end are easier to use instruments that make flow cytometry more accessible to researchers.
8 Time-Saving FACSDiva Software Tips
BD Biosciences brand of digital flow cytometers, including the FACSCanto, the LSR-II, FACSAria and Fortessa, utilize a software acquisition program known as FACSDiva.
Diva is aptly named as it can be a difficult program to master.
However, Diva has come along way over the past 10 years and many improvements have been made to help end-user.
Taking time to learn these changes will improve the reproducibility of your data, the chances of your data getting published, and your overall experience on the cytometer.
These changes will... Read More
6 Tips For Applying The Right Statistical Test To Your Flow Cytometry Data
Flow cytometry data are numbers rich.
Data from experiments can be population measurements (percent of CD4+ cells, for example), or it can be expression level (median fluorescent expression of CD69 on activated T cells).
Many times, researchers are content to show histograms to illustrate their point after a flow experiment. This approach misses the opportunity to take that content rich data and extend the analysis into a statistical analysis.
To properly perform statistical analysis, the first step is to understand the hypothesis. The... Read More