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3 Experiments You Can Do Easier On An ImageStream Flow Cytometer

What happens if one combines the power and speed of traditional flow cytometers with the resolution of a microscope? Cytometry is the study of biological processes at the whole cell level and includes techniques like light microscopy and electron microscopy. But microscopy by itself is a bit different. From the earliest days of microscopy, including the use of the first true microscopes by van Leeuwenhoek and others, scientists have been able to start seeing the finest details of a cell. With the development of the flow cytometer, researchers have been able to explore cellular processes in great deal.  For example, modern flow cytometry technology allows ...

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How To Create The Right Flow Cytometry Antibody Panel Every Time

Sudoku puzzles seem to be all the rage. I see it in coffeehouses, at the airport, even in doctors offices. Everyone is trying to work out how to fit the numbers into the grids so that everything adds up properly. Designing polychromatic flow cytometry panels is much like the Sudoku puzzle. In this case, the grid is composed of the antigens on one side, and the cytometer detectors on the other. The goal is to fill in the grid correctly.   Instead of adding up to 45, like in Sudoku, the flow cytometrist is trying to optimize the ability to make a sensitive measurement to ...

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Why You Should Never Manually Compensate Your Data

(Figure courtesy of Pratip K. Chattopadhyay, Ph.D.) One of the most important steps in proper flow cytometry is the process of compensation. There are a lot of rumors and mysteries that fill laboratory notebooks about the process. Some of these processes are correct while others lead to incorrect compensation, resulting in poor data. Compensation is the process for correcting for the spillover. Spillover is the overlap of a fluorochrome into a second channel due to the physics of fluorescence. This is a mathematical value that ensures that contributions from the fluorochromes not being displayed are not affecting the distribution of the data being displayed. One of the ...

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5 Flow Cytometry Errors Reviewers Despise

We all know that flow cytometry makes individual measurements on large populations of cells, it allows for statistical analysis of the data, lending strength to a researcher's conclusions. Likewise, the isolation of very complex populations by flow cytometry cell sorting can help lead to a richer understanding of the intricate biology at the genomic, proteomic and functional level. As a reviewer of papers and grants, I am always especially interested in the details of HOW the experiments were performed because that is the critical foundation for what the data is able to tell us--and what it can NOT tell us. Mistakes That Mark ...

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The Most Common Mistake Researchers Make When Designing Flow Cytometry Antibody Panels

Pairing highly expressed antigens (like CD3) with dimmer fluorochromes, and the antigens of interest with the brightest fluorochromes, is a key part of panel design with few tools to help. With early generation instruments, this was relatively easy to determine, since fluorochrome choice was limited. With the advent of instruments capable of measuring more than 4 fluorochromes, there is a need to characterize the relative brightness of different fluorochromes under actual experimental conditions, rather than as free fluors. Bigos et al (2004) first reported this in an abstract and it was later simplified in Maecker et al (2004). This equation (Figure 1) compares the  ...

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WELCOME TO EXCYTE

ExCyte is the world’s leading flow cytometry training company. Whether you are a core manger looking to keep your users on the cutting edge, or a business professional looking to enter into this rapidly growing market, ExCyte can help. We have the information and experience you need.

ExCyte is currently booking beginner and advanced flow cytometry courses around the world. Click on the Calendar box above to see where we will be next.

Testimonials
Charlotte Christie Petersen, Ph.D.Core Manager, Aarhus University
I joined ExCyte’s Mastery Class because I wanted to learn a few new flow cytometry methodologies. In particular, I was interested in learning about Cell Cycle and, more broadly, experimental design. I have not done much cell cycle analysis, so I enjoyed and learned a lot from the cell cycle session. Overall, the instructors were excellent and the topics were very relevant. I enjoyed having access to both live webinars and recorded instructional videos. I’m looking forward to see what webinars the Mastery Class offers next.

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I’m a core manager and even though I’m very knowledgeable in the field of flow cytometry, it can be difficult to stay up to date with current trends and current best practices. Occasionally, a new technique or methodology will come up that I want to learn and that’s why I joined ExCyte’s Mastery Class. My favorite thing about the Mastery Class program was the easy-to-follow presentations. They were loaded with many useful tips about flow cytometry and antibody panel design. The program reviews the current best practices in flow cytometry along with a number of other interesting and relevant topics in the field. The instructors were great and the cost was reasonable, what more could you ask for?

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Dorothy Iwanowicz, Ph.D.Sr. Research Associate, Epizyme
I work in R&D and was just a beginner in flow cytometry. I joined ExCyte’s Mastery Class because I needed to learn a lot about flow and I needed to learn it fast. I was amazed at the level of information and the quality of speakers that the program offered. I learned a lot about the entire field of flow and was able to dive deeper into topics I was really interested in like antibody panel design. The Mastery Class is a great program and I would recommend it to other people who want to learn more about flow cytometry.

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Amrutesh Puranik, Ph.D.Postdoctoral Fellow, Mayo Clinic
I’m a postdoctoral researcher and know a lot about flow but I needed to learn a few new techniques and execute them before I could get my paper published. I joined ExCyte’s Mastery Class because they have a good reputation for teaching high level flow cytometry information very well. I really enjoyed the personal guidance the Mastery Class program offered and was impressed by how much I learned about controls and compensation, experimental design, statistics, and even apoptosis. ExCyte’s Mastery Class is perhaps the only class that teaches you from scratch how to be a flow cytometry expert.

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I’m a Principle Investigator and Core Manager. I’m very experienced in flow cytometry and spend a lot of time learning and training to stay on the cutting edge of the field. I joined ExCyte’s Mastery Class to do just that—to keep up with current trends and best practices. Plus, I was interested in learning more about specific things like statistical analysis and measuring proliferation by flow cytometry. I really enjoyed ExCyte’s Mastery Class training videos and was very happy to find that I could go back through the material whenever I wanted because everything was archived. The Mastery Class is a great resource for both beginner and advanced flow cytometry professionals.

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