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5 Flow Cytometry Errors Reviewers Despise

We all know that flow cytometry makes individual measurements on large populations of cells, it allows for statistical analysis of the data, lending strength to a researcher's conclusions. Likewise, the isolation of very complex populations by flow cytometry cell sorting can help lead to a richer understanding of the intricate biology at the genomic, proteomic and functional level. As a reviewer of papers and grants, I am always especially interested in the details of HOW the experiments were performed because that is the critical foundation for what the data is able to tell us--and what it can NOT tell us. Mistakes That Mark ...

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The Most Common Mistake Researchers Make When Designing Flow Cytometry Antibody Panels

Pairing highly expressed antigens (like CD3) with dimmer fluorochromes, and the antigens of interest with the brightest fluorochromes, is a key part of panel design with few tools to help. With early generation instruments, this was relatively easy to determine, since fluorochrome choice was limited. With the advent of instruments capable of measuring more than 4 fluorochromes, there is a need to characterize the relative brightness of different fluorochromes under actual experimental conditions, rather than as free fluors. Bigos et al (2004) first reported this in an abstract and it was later simplified in Maecker et al (2004). This equation (Figure 1) compares the  ...

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Top Flow Cytometry Instruments, Reagents & Software To Look Forward To In 2015 (1st Annual List)

Here at ExCyte--we are excited for all of the new flow cytometry instruments, reagents, and software you will have access to in 2015. There are so many new and innovative products that we have been testing and looking into for you. These are just a few highlights of the great things coming your way in 2015.   I. Instrumentation The field of flow cytometry is seeing various trends in instrumentation--on one end are smaller, more compact and more powerful instruments for the high end needs of researchers, and on the other end are easier to use instruments that make flow cytometry more accessible to ...

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8 Time-Saving FACSDiva Software Tips

8 Time-Saving FACSDiva Software Tips

BD Biosciences brand of digital flow cytometers, including the FACSCanto, the LSR-II, FACSAria and Fortessa, utilize a software acquisition program known as FACSDiva. Diva is aptly named as it can be a difficult program to master. However, Diva has come along way over the past 10 years and many improvements have been made to help end-user. Taking time to learn these changes will improve the reproducibility of your data, the chances of your data getting published, and your overall experience on the cytometer. These changes will also save you time. Here are 8 time-saving FACSDiva tips to use the during your next flow experiment: 1. ...

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6 Tips For Applying The Right Statistical Test To Your Flow Cytometry Data

6 Tips For Applying The Right Statistical Test To Your Flow Cytometry Data

Flow cytometry data are numbers rich. Data from experiments can be population measurements (percent of CD4+ cells, for example), or it can be expression level (median fluorescent expression of CD69 on activated T cells). Many times, researchers are content to show histograms to illustrate their point after a flow experiment. This approach misses the opportunity to take that content rich data and extend the analysis into a statistical analysis. To properly perform statistical analysis, the first step is to understand the hypothesis. The hypothesis will guide the statistical analysis, identifying the correct test to be performed. There are several things that need to ...

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WELCOME TO EXCYTE

ExCyte is the world’s leading flow cytometry training company. Whether you are a core manger looking to keep your users on the cutting edge, or a business professional looking to enter into this rapidly growing market, ExCyte can help. We have the information and experience you need.

ExCyte is currently booking beginner and advanced flow cytometry courses around the world. Click on the Calendar box above to see where we will be next.

Testimonials
Charlotte Christie Petersen, Ph.D.Core Manager, Aarhus University
I joined ExCyte’s Mastery Class because I wanted to learn a few new flow cytometry methodologies. In particular, I was interested in learning about Cell Cycle and, more broadly, experimental design. I have not done much cell cycle analysis, so I enjoyed and learned a lot from the cell cycle session. Overall, the instructors were excellent and the topics were very relevant. I enjoyed having access to both live webinars and recorded instructional videos. I’m looking forward to see what webinars the Mastery Class offers next.

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I’m a core manager and even though I’m very knowledgeable in the field of flow cytometry, it can be difficult to stay up to date with current trends and current best practices. Occasionally, a new technique or methodology will come up that I want to learn and that’s why I joined ExCyte’s Mastery Class. My favorite thing about the Mastery Class program was the easy-to-follow presentations. They were loaded with many useful tips about flow cytometry and antibody panel design. The program reviews the current best practices in flow cytometry along with a number of other interesting and relevant topics in the field. The instructors were great and the cost was reasonable, what more could you ask for?

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Dorothy Iwanowicz, Ph.D.Sr. Research Associate, Epizyme
I work in R&D and was just a beginner in flow cytometry. I joined ExCyte’s Mastery Class because I needed to learn a lot about flow and I needed to learn it fast. I was amazed at the level of information and the quality of speakers that the program offered. I learned a lot about the entire field of flow and was able to dive deeper into topics I was really interested in like antibody panel design. The Mastery Class is a great program and I would recommend it to other people who want to learn more about flow cytometry.

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Amrutesh Puranik, Ph.D.Postdoctoral Fellow, Mayo Clinic
I’m a postdoctoral researcher and know a lot about flow but I needed to learn a few new techniques and execute them before I could get my paper published. I joined ExCyte’s Mastery Class because they have a good reputation for teaching high level flow cytometry information very well. I really enjoyed the personal guidance the Mastery Class program offered and was impressed by how much I learned about controls and compensation, experimental design, statistics, and even apoptosis. ExCyte’s Mastery Class is perhaps the only class that teaches you from scratch how to be a flow cytometry expert.

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I’m a Principle Investigator and Core Manager. I’m very experienced in flow cytometry and spend a lot of time learning and training to stay on the cutting edge of the field. I joined ExCyte’s Mastery Class to do just that—to keep up with current trends and best practices. Plus, I was interested in learning more about specific things like statistical analysis and measuring proliferation by flow cytometry. I really enjoyed ExCyte’s Mastery Class training videos and was very happy to find that I could go back through the material whenever I wanted because everything was archived. The Mastery Class is a great resource for both beginner and advanced flow cytometry professionals.

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