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What Is Fluorescent Activated Cell Sorting And 4 Other Questions About FACS Data Analysis

Written By Tim Bushnell, PhD Prior to the mid-1960’s, the ability to study a defined cell type was severely limited. Researchers had to use centrifugation methods, such as differential centrifugation, rate zonal centrifugation, or isopycnic centrifugation, to define cell types. All of these methods would allow separation of cells based on the property of the particles within different separation medias, but didn’t allow for very fine resolution of the cell populations.  That all changed starting in the mid-1960’s, when Mack Fulwyler published the first true cell sorter, which combined the power of cell characterization by the Coulter principle with the electrostatic separation of droplets ...

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4 Biggest Mistakes Scientists Make During Multicolor Flow Cytometry Cell Sorting Experiments

Written By Mike Kissner Multicolor cell sorting is a complicated process and certain scientific errors can be common. Unsuccessful multicolor sorts can result in erroneous data and inconclusive results. Successful multicolor sorts, on the other hand, can give excellent results and lead to dynamic conclusions. Successful multicolor cell sorting requires special attention to planning. Using specific setup strategies for your experiment can create a streamlined system for an otherwise complicated process. For example, these critical steps and strategies for multicolor sorting experiments can save you time and maximize your results. When setting up a multicolor experiment, the most common mistakes are failing to set PMT ...

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5 Gating Strategies To Get Your Flow Cytometry Data Published In Peer-Reviewed Scientific Journals

Written by Tim Bushnell, PhD “Every block of stone has a statue inside it and it is the task of the sculptor to discover it.” — Michelangelo When sitting down to perform a new analysis of flow cytometry data, it is much like Michelangelo staring at a piece of marble. There is a story inside the data, and it is the job of the researcher to unravel it. The critical difference between sculptor and scientist is that while the sculptor is guided by a creative vision, the researcher is guided by very particular laws of nature and a specific method of working through ...

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How To Create A Flow Cytometry Quality Assurance Protocol For Your Lab

By EditaMotyčáková, Ph.D. Editor's note: This is based on Edita’s experiences implementing the proposals reported in Perfetto et al., (2012) Nature Protocols 7:2067.  She submitted this to the ExCyte Mastery Class and developed the Excel sheet (attached) to assist others in tracking their QC data. Developing and implementing a new Quality Control (QC) protocol can sometimes be a daunting task. With the continued emphasis on reproducibility in science, QC programs are an essential step that cytometrists are encouraged to both develop and implement. This QC program comprises several assays that are focused on three important characteristics of the flow cytometer: (1) Optimal instrument setting (e.g. instrument ...

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How To Analyze FACS Data And Prepare Flow Cytometry Figures For Scientific Papers

Written by Tim Bushnell, PhD “It would be possible to describe everything scientifically, but it would make no sense; it would be without meaning, as if you described a Beethoven symphony as a variation of wave pressure.” ― Albert Einstein   The goal of any scientific process, as you know, requires the communication of the data that supports or refutes the hypothesis under testing. Before it is deemed worthy of publication, it must survive the process of peer review ― where the data is laid bare before a group of experts in the field who judge the material impartially (usually) and in secret ― then ...

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WELCOME TO EXCYTE

ExCyte is the world’s leading flow cytometry training company. Whether you are a core manger looking to keep your users on the cutting edge, or a business professional looking to enter into this rapidly growing market, ExCyte can help. We have the information and experience you need.

ExCyte is currently booking beginner and advanced flow cytometry courses around the world. Click on the Calendar box above to see where we will be next.

Testimonials
MedImmune purchased a site license of Expert Cytometry’s Mastery Class. We have over 100 scientists using the program and the feedback has been phenomenal. I’m amazed at how much the scientists are coming to me with questions that are much more advanced than what I used to get before having access to the Mastery Class. I’m now able to focus my attention, and that of my staff’s, to more advanced questions and inquiries specific to the researcher. We are also having less trouble with the mistakes that new users make and spending less time dealing with troubleshooting basic issues. As the core director at MedImmune, I definitely recommend the Expert Cytometry Mastery Class to all cores, particularly in a corporate setting, where we simply do not have the time to train all of the new users in best practices. My core is running 10 hours a week, ...

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Johnna Wesley, Ph.D.Senior Manager, Novo Nordisk
Expert Cytometry provided an excellent course to our group. The course was over two days. On day one, the instructors provided a great overview of core concepts of flow. On day two, we were able to customize the course to our needs. It was very valuable and I would recommend that if your group is starting to use flow more heavily, that you bring Expert Cytometry in for a course. There are nuances that I never knew and it immediately helped our group perform better experiments.   

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I was (and still am for the most part) completely ignorant of the details of flow cytometry. The Expert Cytometry team provided explanations and helped me understand the technology to the point where I was able to intelligently and efficiently manage both of our contract flow labs. Expert Cytometry provided independent assessments of the quality of the work and data from the labs that allowed me to make good decisions regarding the results. Finally, I was always amazed at how quickly Expert Cytometry responded and completed the analyses when we were on short timelines. We continue to work with ExCyte and will for the foreseeable future.

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Our group at BlueBirdBio was relatively new to flow. We brought ExCyte in for a basic course, and also to help train us on best practices for our new flow cytometer. The level of flow cytometry knowledge ExCyte brought with them was amazing. I can’t believe how much experience ExCyte has and how much they bring to the courses they provide. ExCyte was able to answer all questions with real world examples. The course brought the theory of flow to the real world and helped us understand the concepts more easily. ExCyte was professional, courteous, and it almost felt like they became part of our group while we were working together. I know I can ask ExCyte any questions whenever I need them and I hope to continue to work with them as we move forward. I have no doubt we’ll bring ExCyte back for another course in ...

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We worked with Expert Cytometry for one year, utilizing them for consulting work and writing technical documents for several of our flow cytometry products. Working with Tim Bushnell at Expert Cytometry was great. Tim is efficient and highly technical. His 15+ years of experience really shows, as he brings a level of detail to the work that we had never experienced from other consultants. There are a lot of so-called flow cytometry “experts” out there, but having worked with numerous ones, we have found the group at Expert Cytometry to be the most knowledgeable. As we continue to expand our flow cytometry portfolio, I will definitely work with Tim and his group at Expert Cytometry again.

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