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What Is MIFlowCyt And The FlowRepository, Or Why Flow Cytometry Is Being Standardized

Written by Ryan Brinkman, Ph.D. Flow cytometry is a powerful screening tool that can be used to help identify target candidates on phenotypically defined cell populations. There are a myriad of possible assays that can be combined using flow cytometry to examine the effects on these cells. Not to mention the fact that millions of cells can be measured in one experiment, or the statistical power of flow cytometry. Of course, for these assays to be robust and reproducible within an individual lab, adherence to standard operating procedures and current best practices in flow cytometry is required. New dyes, new protocols, and new reagents ...

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5 Experimental Errors That Prevent Your Flow Cytometry Data From Passing The Peer Review Process

Written by Tim Bushnell, Ph.D. Reproducibility is a critical component of the scientific process. One cannot publish data if the experiments cannot be replicated. Unfortunately, as Begley and Ellis pointed out in a commentary in Nature that when Amgen attempted to reproduce 53 “landmark” papers in the area of cancer research, only 6 papers were “confirmed.” What does that mean to you, the flow cytometry researcher? To avoid publishing errors that reviews despise, it’s important to follow and promote the best practices in the field, thus ensuring that your data is reproducible to investigators attempting to validate your research. In particular, you must follow these ...

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How To Differentiate T-Regulatory Cells (Tregs) By Flow Cytometry

Written by Jennifer Snyder-Cappione, Ph.D. T-regulatory cells (Tregs), formerly known as T suppressor cells, are a T cell subset with direct roles in both autoimmunity and responses to pathogens. Tregs decrease inflammation via the secretion of immunosuppressive cytokines (IL-10, TGF-b) and also through direct suppression of inflammatory effector T cells (such as Th1 and Th17 cells). Tregs control and likely prevent autoimmune diseases by contributing to the maintenance of tolerance to self-antigens. The therapeutic benefit of Treg transfer is well established in animal models and efforts to begin human Treg therapies are underway for transplantation and Type 1 diabetes patients. Given the importance of ...

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Increase Cell Viability With These 3 Flow Cytometry Experimental Research Design Tips

By Michael Kissner The cell sorting process, while generally well-tolerated by most cell types, is inherently stressful. Cells are first manipulated in suspension for up to several hours to prepare and stain them. Then, during the cell sorting process, these cells are pushed through narrow tubing under high pressure in the range of approximately 10-70 PSI, rapidly depressurized after passing through a nozzle, and then jetted through the air at velocities of 20 m/s (~44 MPH) or higher. Keeping cells healthy, happy, vital, and viable over the course of a cell sorting experiment is important, not only to keep cells alive during the sort, ...

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What Is The Coulter Principle And Why You Need To Obtain Complete Blood Counts By Flow Cytometry

Written by James McCracken, Ph.D. When you get your blood drawn for your annual physical, or if you have been hospitalized, there are some common flow cytometry tests that your Medical Technician will run. These tests will often analyze your blood lipids, vitamin levels, ion concentrations, and more often than not, your complete blood count (CBC). The CBC is an automated hematology test (really several tests being run in the same instrument) that looks at the levels of all the cells in your blood, providing your physician with valuable information about your health. The CBC is a powerful addition to many flow cytometry workflows.  Using ...

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WELCOME TO EXCYTE

ExCyte is the world’s leading flow cytometry training company. Whether you are a core manger looking to keep your users on the cutting edge, or a business professional looking to enter into this rapidly growing market, ExCyte can help. We have the information and experience you need.

ExCyte is currently booking beginner and advanced flow cytometry courses around the world. Click on the Calendar box above to see where we will be next.

Testimonials
MedImmune purchased a site license of Expert Cytometry’s Mastery Class. We have over 100 scientists using the program and the feedback has been phenomenal. I’m amazed at how much the scientists are coming to me with questions that are much more advanced than what I used to get before having access to the Mastery Class. I’m now able to focus my attention, and that of my staff’s, to more advanced questions and inquiries specific to the researcher. We are also having less trouble with the mistakes that new users make and spending less time dealing with troubleshooting basic issues. As the core director at MedImmune, I definitely recommend the Expert Cytometry Mastery Class to all cores, particularly in a corporate setting, where we simply do not have the time to train all of the new users in best practices. My core is running 10 hours a week, ...

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Johnna Wesley, Ph.D.Senior Manager, Novo Nordisk
Expert Cytometry provided an excellent course to our group. The course was over two days. On day one, the instructors provided a great overview of core concepts of flow. On day two, we were able to customize the course to our needs. It was very valuable and I would recommend that if your group is starting to use flow more heavily, that you bring Expert Cytometry in for a course. There are nuances that I never knew and it immediately helped our group perform better experiments.   

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I was (and still am for the most part) completely ignorant of the details of flow cytometry. The Expert Cytometry team provided explanations and helped me understand the technology to the point where I was able to intelligently and efficiently manage both of our contract flow labs. Expert Cytometry provided independent assessments of the quality of the work and data from the labs that allowed me to make good decisions regarding the results. Finally, I was always amazed at how quickly Expert Cytometry responded and completed the analyses when we were on short timelines. We continue to work with ExCyte and will for the foreseeable future.

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Our group at BlueBirdBio was relatively new to flow. We brought ExCyte in for a basic course, and also to help train us on best practices for our new flow cytometer. The level of flow cytometry knowledge ExCyte brought with them was amazing. I can’t believe how much experience ExCyte has and how much they bring to the courses they provide. ExCyte was able to answer all questions with real world examples. The course brought the theory of flow to the real world and helped us understand the concepts more easily. ExCyte was professional, courteous, and it almost felt like they became part of our group while we were working together. I know I can ask ExCyte any questions whenever I need them and I hope to continue to work with them as we move forward. I have no doubt we’ll bring ExCyte back for another course in ...

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We worked with Expert Cytometry for one year, utilizing them for consulting work and writing technical documents for several of our flow cytometry products. Working with Tim Bushnell at Expert Cytometry was great. Tim is efficient and highly technical. His 15+ years of experience really shows, as he brings a level of detail to the work that we had never experienced from other consultants. There are a lot of so-called flow cytometry “experts” out there, but having worked with numerous ones, we have found the group at Expert Cytometry to be the most knowledgeable. As we continue to expand our flow cytometry portfolio, I will definitely work with Tim and his group at Expert Cytometry again.

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