Flow Cytometry Training | Expert Cytometry | Master The Art of Cytometry Flow Cytometry Training | Expert Cytometry | Master The Art of Cytometry

We Provide Flow Cytometry Training To
Academics And Industry Professionals

From The Blog

Flow Cytometry Protocols To Prevent Sample Clumping

Written By Tim Bushnell, Ph.D. Good flow cytometry depends on a high-quality, single cell suspension. If the cells put through the instrument are not of high quality, the ensuing data will be difficult to analyze. Likewise, if the sample is clumpy, one will not be able to readily distinguish cells of interest from the clumps they are attached to. Thus, sample preparation becomes the critical first step in any flow cytometry experiment. As Henry Ford once said, “Before everything else, getting ready is the secret of success.” For the purposes of this article, our attention will be focused on mammalian cells and cell-lines. ...

Read More

How Flow Cytometry Optical System Components Work

Written by Tim Bushnell, Ph.D. The importance of the optical system of your flow cytometer was established in Part I of this series, noting in particular the benefits of foundational knowledge to gain a comprehensive view of your data, as well as troubleshooting throughout your experiment. In Part I, the role of lasers was broken down. This follow-up article investigates the lenses, mirrors, and filters in your flow cytometer. To review these three elements... Lasers illuminate the stream with coherent, focused light of specific wavelength (energy) and power. This illumination facilitates the generation of fluorescence signals from cells labeled with fluorophores and light scatter ...

Read More

What Is A Flow Cytometry Laser And How Flow Cytomtery Optics Function

Written by Tim Bushnell, PhD The optical system of a flow cytometer consists of an elegant coordination of many components that function concordantly and synchronously to generate the signals that we need to measure in order to shed light on the biology at hand. Understanding the optical system of a flow cytometer may seem unnecessary for performing a typical experiment, but the more you know about your instrument, the better you will be at understanding nuanced aspects of your data, as well as troubleshooting any potential issues that may arise during an experiment. A cytometer’s optical system can be broken down into two ...

Read More

4 Gating Controls Your Flow Cytometry Experiment Needs To Improve Reproducibility

Written by Tim Bushnell, PhD To reproduce reliably in flow cytometry, one must control the gate. The identification of the target cells of an experiment is the critical first step to performing the secondary analysis that will be used to judge the biological hypothesis and is done by peeling away the layers of cells that do not meet the criteria. This involves the data reduction method of ‘gating’ with the researcher as gatekeeper, controlling what may pass and what shall not pass, based on the controls designed for the specific experiment. It is disappointing to realize that in the paper, Maecker et al., the ...

Read More

How To Improve Reproducibility Through The Automated Analysis Of Flow Cytometry Data

Written by Ryan Brinkman, Ph.D. Editor's Note:  Reproducibility continues to be a critical area that all researchers need to be aware of. From the NIH’s focus on reproducibility in grant applications, to a renewed focus by reviewers on the way data has been analyzed and presented, it is imperative that researchers keep up on best practices to ensure they pass these hurdles.  One area that flow cytometry researchers should be focusing on is the emerging changes in the area of automated data analysis. Over the last five years there have been dramatic changes and improvements in these programs and workflows. As ...

Read More

WELCOME TO EXCYTE

ExCyte is the world’s leading flow cytometry training company. Whether you are a core manger looking to keep your users on the cutting edge, or a business professional looking to enter into this rapidly growing market, ExCyte can help. We have the information and experience you need.

ExCyte is currently booking beginner and advanced flow cytometry courses around the world. Click on the Calendar box above to see where we will be next.

Testimonials
MedImmune purchased a site license of Expert Cytometry’s Mastery Class. We have over 100 scientists using the program and the feedback has been phenomenal. I’m amazed at how much the scientists are coming to me with questions that are much more advanced than what I used to get before having access to the Mastery Class. I’m now able to focus my attention, and that of my staff’s, to more advanced questions and inquiries specific to the researcher. We are also having less trouble with the mistakes that new users make and spending less time dealing with troubleshooting basic issues. As the core director at MedImmune, I definitely recommend the Expert Cytometry Mastery Class to all cores, particularly in a corporate setting, where we simply do not have the time to train all of the new users in best practices. My core is running 10 hours a week, ...

Read More

Johnna Wesley, Ph.D.Senior Manager, Novo Nordisk
Expert Cytometry provided an excellent course to our group. The course was over two days. On day one, the instructors provided a great overview of core concepts of flow. On day two, we were able to customize the course to our needs. It was very valuable and I would recommend that if your group is starting to use flow more heavily, that you bring Expert Cytometry in for a course. There are nuances that I never knew and it immediately helped our group perform better experiments.   

Read More

I was (and still am for the most part) completely ignorant of the details of flow cytometry. The Expert Cytometry team provided explanations and helped me understand the technology to the point where I was able to intelligently and efficiently manage both of our contract flow labs. Expert Cytometry provided independent assessments of the quality of the work and data from the labs that allowed me to make good decisions regarding the results. Finally, I was always amazed at how quickly Expert Cytometry responded and completed the analyses when we were on short timelines. We continue to work with ExCyte and will for the foreseeable future.

Read More

Our group at BlueBirdBio was relatively new to flow. We brought ExCyte in for a basic course, and also to help train us on best practices for our new flow cytometer. The level of flow cytometry knowledge ExCyte brought with them was amazing. I can’t believe how much experience ExCyte has and how much they bring to the courses they provide. ExCyte was able to answer all questions with real world examples. The course brought the theory of flow to the real world and helped us understand the concepts more easily. ExCyte was professional, courteous, and it almost felt like they became part of our group while we were working together. I know I can ask ExCyte any questions whenever I need them and I hope to continue to work with them as we move forward. I have no doubt we’ll bring ExCyte back for another course in ...

Read More

We worked with Expert Cytometry for one year, utilizing them for consulting work and writing technical documents for several of our flow cytometry products. Working with Tim Bushnell at Expert Cytometry was great. Tim is efficient and highly technical. His 15+ years of experience really shows, as he brings a level of detail to the work that we had never experienced from other consultants. There are a lot of so-called flow cytometry “experts” out there, but having worked with numerous ones, we have found the group at Expert Cytometry to be the most knowledgeable. As we continue to expand our flow cytometry portfolio, I will definitely work with Tim and his group at Expert Cytometry again.

Read More

Flow Cytometry Education And Consulting - Affordable. Effective. Leading Edge.