• Flow Cytometry Education and Consulting - Affordable. Effective. Leading Edge.

    How To Design Accurate & Effective Flow Antibody Panels (or, What’s An OMIP?)

    How To Design Accurate & Effective Flow Antibody Panels (or, What's An OMIP?)

    I was at a meeting talking about the principles of panel design. At the end of my talk, I had an investigator approach me and ask why he was not making progress on his 15-color panel that he started developing. So, I asked how long he’d been working on it. A month. That was his response. This might shock some of you but a month is not very long when it comes to designing an accurate and effective antibody panel for a flow cytometry experiment. Multicolor panel design requires a delicate balance of biology and physics. Understanding the biology of the system and ...

    Read More

    10 FlowJo Version X Hacks That Will Help You Publish Your Flow Cytometry Data

    So you just got the most amazing results of your life and you can wait to show it off in lab meeting, or create the figure for a publication. Here are a couple of tips that will help you ensure that everyone else also sees how amazing your data is! 1. If you only have a few events, use the option to “show large dots”. When you only have 4-5 events in a population, it can often be difficult to see. If you turn off the high resolution, you can see the data better. Double click on a plot in the layout editor ...

    Read More

    Top 10 Flow Cytometry Resources

    I've been in the world of flow cytometry and cell sorting for a very long time. Now, don’t worry, this isn’t going to be some lament about the “good old days.” Well, maybe just a little. But there will be helpful takeaways, I promise. I was trained by the incredible staff in the core facility at the University of Arizona. When I moved to UC Davis and proceeded to start up a core facility, there were very few resources to guide me. No Google communities and LinkedIn groups. Email was new, there was the Purdue Cytometry List, but that was about ...

    Read More

    4 Fluidics Tips That Will Change Your Flow Data For The Better

    Friday is the 4th of July in the US - and we celebrate that day with picnics, spending time OUTSIDE the lab and fireworks. And our Independence, but I'm not up for a political discussion right now. For flow geeks, fireworks are like flow cytometry - they happen in the dark, they are full of many bright colors, and we’re all looking for the patterns the colors make. So in honor of a day outside the lab, it seemed appropriate to talk about fluidics... going with the flow for best results. A flow cytometer has three major components - fluidics, electronics ...

    Read More

    Counting Cells Will Save Your Flow Cytometry Experiment, If You Do It Like This

    Fast, accurate or efficient - pick two. How to decide when you can’t have it all. The hemocytometer is considered the gold standard for cell counting. Invented by Louis Charles Malassez, this precision etched microscope slide can allow the researcher to count their cells under the microscope with amazing accuracy. It is inexpensive, relative to other methods, but is by no means the most efficient or fast method out there. The single biggest key to using a hemocytometer is training, training and more training. Since the investigator is visually inspecting the cells within a boundary, the rules of what cells to count ...

    Read More

    WELCOME TO EXCYTE

    ExCyte is the world’s leading flow cytometry training company. Whether you are a core manger looking to keep your users on the cutting edge, or a business professional looking to enter into this rapidly growing market, ExCyte can help. We have the information and experience you need.

    ExCyte is currently booking beginner and advanced flow cytometry courses around the world. Click on the Calendar box above to see where we will be next.

    Robert Fultz Baylor College of Medicine
    I have much more confidence in the quality of data that I’m able to get from my samples after taking the Expert Cytometry, Complex Data Analysis course that was offered in Houston this year. I found the curriculum, the way it was taught, and the hands-on learning to be hugely helpful. I am very new to the world of flow cytometry so proficiency in even the most basic data preparation and analysis is critical for me and this class really put ahead of the curve. Additionally, using the techniques I learned in the course, I re-evaluated some data that I previously collected and found a critical error with the way the samples were run. The error would not have been detected without the knowledge I acquired in the course. I strongly recommend this course for anyone doing even simple FC analysis.

    Read More

    I attended an Excyte course at the University of Iowa. The course was extremely detail-oriented, allowing attendees to work through the process of choosing the correct flow cytometry flurophores and reagents for their particular experiments. I learned a lot from the discussions on flow cytometry controls, which helped me figure out which ones to use in my own research.  I found the instructor very approachable and willing to stay after the lecture for a long time to answer questions and give additional resources.

    Read More

    I invited ExCyte to Lilly to teach a two-day comprehensive course; ranging from the fundamentals of flow to the experimental design of assays specific to what I think a lot of biotech companies are doing these days. Afterwards, several of my users wondered why I didn’t bring in a course like this earlier! Our instructor, Tim Bushnell, Ph.D., educated my users with the background they needed to design their assays most effectively. I immediately noticed the positive impact he had when my users began to ask more informed questions during the planning stages of their experiments. As a busy core manager, it’s difficult to find the time to prepare such a well designed course like ExCyte offers while trying to stay focused on innovation and maintaining a competitive advantage in the fast paced industry environment. I’m grateful to the ExCyte team for helping me to keep things moving forward at ...

    Read More

    Excyte provided a wonderful course in Boston.  Feedback from attendees has been very positive and individuals want more. I believe there is great benefit to advanced courses in Flow Cytometry as number of fluorescence antibodies and analysis parameters keeps increasing. Between the multi-institutional CyTOF, and my labs 32 parameter Astrios, we are moving into some very high-end and cutting edge science. The expertise that the ExCyte team brings to these courses is incredible. It is amazing how some individuals such as ExCyte can take very complex materials and make them appear simple. In addition, I am and always will be a strong advocate of learning by doing. ExCyte's course structure allows the student to be involved and interactive. This allows for greater ease of translation of the learned material into the student's experiments. Great courses from great people.

    Read More

    As a core manager, ExCyte has been great to work with.  It's often difficult to ensure clients are getting the information and education they need to perform their experiments effectively.  We have worked with Carol and Tim several times to deliver effective educational programs to our users.  They have a broad offering of classes and are willing to work with us to tailor the topics to our needs.  Client feedback has been very good which is a testament to the knowledge and expertise of the people behind ExCyte. I also like their new series of webinars which are inexpensive, informative and allow users to gain valuable knowledge one hour at a time.

    Read More

    BD
    Miltenyi
    Bio-RAd
    FlowJo
    Cytometry Consulting and Education - Affordable. Effective. Leading Edge.