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7 Advanced Flow Cytometry Data Analysis Tips For Multi-Color Experiments

There was a time when two- and three-color experiments were considered very complex flow cytometry experiments. A limited number of dyes and light sources meant a limited number of options overall.  In fact, the first experiments sorting cells with fluorescently-labeled antibodies were performed by Len Herzenberg in 1972 and could only detect fluorescence from an Argon laser source above 530 nm. These early experiments were performed using rhodamine- and fluorescein-tagged antibodies. Times have changed. Now, we have spatially separated lasers and a seemingly unlimited number of dyes. From 2-Colors To 10-Colors Not long after Herzenberg’s work, Howard Shapiro began designing a series of multiparameter instruments. ...

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Why Understanding The Jablonski Diagram Will Help You Publish Your Flow Cytometry Data

We are all used to interruptions during our working day, from the ping of an email notification to the knock of a fellow researcher who wants to troubleshoot their experiment. Fortunately, most of these interruptions only last a few minutes. Some past researchers were not so lucky. Imagine your work being interrupted by a war. Imagine it being interrupted by two wars that you had to fight in. Alexander Jablonski often had his studies interrupted, not by emails or colleagues, but by war. Jablonski’s work was held up for years due to military service in two wars. First, he served in the war ...

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When To Use (And Not Use) Flow Cytometry Isotype Controls

Antibodies can bind to cells in a specific manner - where the FAB portion of the antibody binds to a high-affinity specific target or the FC portion of the antibody binds to the FcR on the surface of some cells. They can also bind to cells in a nonspecific manner, where the FAB portion binds to a low affinity, non-specific target. Further, as cells die, and the membrane integrity is compromised, antibodies can non-specifically bind to intracellular targets. The question has always been how to identify and control for the nonspecific antibody binding observed.   This led to many research groups using a control called ...

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6 Keys To Running A Proper Clinical Flow Cytometry Experiment

Clinical use of flow cytometry has paralleled the development of instrumentation and reagents. One early application for flow cytometry is the measurement of DNA content.  Malignancies and neoplasms often have abnormal amounts of DNA, and this can be assessed with a variety of protocols and dyes. Comparing DNA Index (DI) of a known 2N control to a sample can yield useful information, but the clinical application of this information was limited, as it was not known how it turned these data into meaningful biological and clinical data insights. The Rise Of Clinical Flow Cytometry With the increased development of fluorescently conjugated monoclonal antibodies came more ...

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How To Perform A SPICE Analysis With FlowJo

Flow cytometry data analysis is getting more complex. Gone is the rule of 2-3 color experiments. Even beginners are starting with 5+ color assays, and the adoption of mass cytometry has the potential to increase our headaches even more. Current data analysis methods are good for single tubes or small cohort studies.  What do you do when you have a large dataset, with multiple sampling conditions, and multiple outcome measurements? With data complexity of this nature, one can export the numerical data to a third party analysis package, but even then the analysis can be difficult to perform. To overcome this limitation, and to allow ...

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WELCOME TO EXCYTE

ExCyte is the world’s leading flow cytometry training company. Whether you are a core manger looking to keep your users on the cutting edge, or a business professional looking to enter into this rapidly growing market, ExCyte can help. We have the information and experience you need.

ExCyte is currently booking beginner and advanced flow cytometry courses around the world. Click on the Calendar box above to see where we will be next.

Testimonials
Charlotte Christie Petersen, Ph.D.Core Manager, Aarhus University
I joined ExCyte’s Mastery Class because I wanted to learn a few new flow cytometry methodologies. In particular, I was interested in learning about Cell Cycle and, more broadly, experimental design. I have not done much cell cycle analysis, so I enjoyed and learned a lot from the cell cycle session. Overall, the instructors were excellent and the topics were very relevant. I enjoyed having access to both live webinars and recorded instructional videos. I’m looking forward to see what webinars the Mastery Class offers next.

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I’m a core manager and even though I’m very knowledgeable in the field of flow cytometry, it can be difficult to stay up to date with current trends and current best practices. Occasionally, a new technique or methodology will come up that I want to learn and that’s why I joined ExCyte’s Mastery Class. My favorite thing about the Mastery Class program was the easy-to-follow presentations. They were loaded with many useful tips about flow cytometry and antibody panel design. The program reviews the current best practices in flow cytometry along with a number of other interesting and relevant topics in the field. The instructors were great and the cost was reasonable, what more could you ask for?

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Dorothy Iwanowicz, Ph.D.Sr. Research Associate, Epizyme
I work in R&D and was just a beginner in flow cytometry. I joined ExCyte’s Mastery Class because I needed to learn a lot about flow and I needed to learn it fast. I was amazed at the level of information and the quality of speakers that the program offered. I learned a lot about the entire field of flow and was able to dive deeper into topics I was really interested in like antibody panel design. The Mastery Class is a great program and I would recommend it to other people who want to learn more about flow cytometry.

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Amrutesh Puranik, Ph.D.Postdoctoral Fellow, Mayo Clinic
I’m a postdoctoral researcher and know a lot about flow but I needed to learn a few new techniques and execute them before I could get my paper published. I joined ExCyte’s Mastery Class because they have a good reputation for teaching high level flow cytometry information very well. I really enjoyed the personal guidance the Mastery Class program offered and was impressed by how much I learned about controls and compensation, experimental design, statistics, and even apoptosis. ExCyte’s Mastery Class is perhaps the only class that teaches you from scratch how to be a flow cytometry expert.

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I’m a Principle Investigator and Core Manager. I’m very experienced in flow cytometry and spend a lot of time learning and training to stay on the cutting edge of the field. I joined ExCyte’s Mastery Class to do just that—to keep up with current trends and best practices. Plus, I was interested in learning more about specific things like statistical analysis and measuring proliferation by flow cytometry. I really enjoyed ExCyte’s Mastery Class training videos and was very happy to find that I could go back through the material whenever I wanted because everything was archived. The Mastery Class is a great resource for both beginner and advanced flow cytometry professionals.

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